NADH氧化酶(NOX)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:NADH Oxidase(NOX) Activity Assay Kit
储存条件:Store at -20℃,6 months


产品简介
中文名称 | NADH氧化酶(NOX)活性检测试剂盒 |
英文名称 | NADH Oxidase(NOX) Activity Assay Kit |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at -20℃,6 months |
规格 | 100T/48S |
单位 | 盒 |
测定原理:
NOX能够将NADH氧化为NAD,NADH的氧化与2,6二氯酚靛蓝(DCPIP)的还原相偶联,蓝色的DCPIP被还原为无色的DCPIP,在600nm下测定蓝色DCPIP的还原速率计算出NADH氧化酶活性的大小。
需要的仪器,耗材:
可见分光光度计/酶标仪、台式离心机、水浴锅、可调式移液器、微量石英比色皿/96孔板、研钵、冰、蒸馏水
测定意义:
NOX(EC 1.6.99.3)广泛存在于动物、植物、微生物和培养细胞中,可在氧气存在下,直接将NADH氧化为NAD。该酶不仅参与NAD的再生,而且与免疫反应密切相关。测定原理:NOX能够将NADH氧化为NAD,NADH的氧化与2,6二氯酚靛蓝(DCPIP)的还原相偶联,蓝色的DCPIP被还原为无色的DCPIP,在600nm下测定蓝色DCPIP的还原速率计算出NADH氧化酶活性的大小。需要的仪器,耗材:可见分光光度计/酶标仪、台式离心机、水浴锅、可调式移液器、微量石英比色皿/96孔板、研钵、冰、蒸馏水
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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