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辅酶ⅠNAD(H)含量检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:CoenzymeⅠNAD(H) Content Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 辅酶ⅠNAD(H)含量检测试剂盒 |
| 英文名称 | CoenzymeⅠNAD(H) Content Assay Kit |
| 检测方法 | 微量法 Spectrophotometer/Microplate Reader |
| 储存条件 | Store at -20℃，6 months |
| 运输条件 | 冷冻运输 |
| 规格 | 100T/96S |
| 单位 | 盒 |
产品说明：
辅酶I包括还原型和氧化型两种形式，在生物氧化中起传递氢的作用。氧化型辅酶I又称烟酰胺腺嘌呤二核苷酸（NAD+）是脱氢酶的辅酶，它在糖酵解、糖异生、三羧酸循环和呼吸链中发挥着不可替代的作用。中间产物会将脱下的氢递给NAD，使之成为NADH（还原型辅酶I）。而NADH则会作为氢的载体，在呼吸链中通过化学渗透偶联的方式，合成ATP。NAD(H)在机体内有重要的生理意义，与物质代谢、能量代谢、抗细胞衰老、抗氧化以及一些疾病的发生密切相关。体内辅酶I含量降低会导致细胞损伤或衰亡。
分别用酸性和碱性提取液提取样本中NAD+和NADH，NADH通过PMS的递氢作用，还原氧化型噻唑蓝（MTT）为甲瓒，在570nm下检测吸光值，而NAD+可被乙醇脱氢酶还原为NADH，进一步采用MTT还原法检测。
需自备的仪器和用品：
可见分光光度计/酶标仪、台式离心机、移液器、微量玻璃比色皿/96孔板、研钵/匀浆器、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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