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NAD-苹果酸脱氢酶(NAD-MDH)活性检测试剂盒
检测方法:紫外分光光度法 Spectrophotometer
英文名称:NAD-Malate Dehydrogenase(NAD-MDH) Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | NAD-苹果酸脱氢酶(NAD-MDH)活性检测试剂盒 |
| 英文名称 | NAD-Malate Dehydrogenase(NAD-MDH) Activity Assay Kit |
| 检测方法 | 紫外分光光度法 Spectrophotometer |
| 储存条件 | Store at -20℃，6 months |
| 规格 | 50T/48S |
| 单位 | 盒 |
测定意义：MDH （EC 1.1.1.37）广泛存在于动物、植物、微生物和培养细胞中，线粒体中MDH 是TCA循环的关键酶之一，催化苹果酸形成草酰乙酸；相反，胞浆中MDH 催化草酰乙酸形成苹果酸。草酰乙酸是重要的中间产物， 连接多条重要的代谢途径。因此，MDH 在细胞多种生理活动中扮演着重要的角色，包括线粒体的能量代谢、苹果酸-天冬氨酸穿梭系统、活性氧代谢和抗病性等。根据不同的辅酶特异性，MDH 分为NAD-依赖的MDH 和NADP-依赖的MDH，细菌中通常只含有NAD-MDH，在真核细胞中，NAD-MDH 分布于细胞质和线粒体中。
测定原理：NAD-MDH 催化NADH 还原草酰乙酸生成苹果酸，导致340nm 处光吸收下降。
需自备的仪器和用品：紫外分光光度计、台式离心机、水浴锅、可调式移液器、1 mL 石英比色皿和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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