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辅酶ⅠNAD(H)含量检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:CoenzymeⅠNAD(H) Content Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 辅酶ⅠNAD(H)含量检测试剂盒 |
| 英文名称 | CoenzymeⅠNAD(H) Content Assay Kit |
| 检测方法 | 可见分光光度法 Spectrophotometer |
| 储存条件 | Store at -20℃，6 months |
| 规格 | 50T/48S |
| 单位 | 盒 |
测定意义：辅酶ⅠNAD(H)广泛存在于动物、植物、微生物和培养细胞中，NAD+是糖酵解（EMP）和三羧酸循环（TCA）的主要氢受体，生成的NADH经呼吸电子链（ETC）传递把电子交给氧，在合成ATP的同时，形成大量的ROS，同时NADH再生为NAD+。糖、脂、蛋白质三大代谢物质分解中的氧化反应绝大部分通过这一体系完成。NAD(H)含量和NADH/NAD+比值的高低可用于评价糖酵解和TCA循环的强弱。较高的NAD(H)及NADH/NAD+比值说明细胞呼吸耗氧量较高，处于过氧化状态。此外，NADH/NAD+比值升高也可抑制糖酵解和TCA循环。另外，NAD+降解产物对细胞信号传导、代谢和基因表达等具有重要的调控作用。
测定原理：分别用酸性和碱性提取液提取样品中NAD+和NADH，NADH通过PMS的递氢作用，还原氧化型噻唑蓝（MTT）为甲瓒，在570nm下检测吸光值；而NAD+可被乙醇脱氢酶还原为NADH，进一步采用MTT还原法检测。
需自备的仪器和用品：可见分光光度计、台式离心机、移液器、1 mL玻璃比色皿、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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