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羟脯氨酸(HYP)含量检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Hydroxyproline(HYP) Content Assay Kit
储存条件:Store at 2-8℃，6 months
产品简介
| 自备试剂 | 该试剂盒实验过程中需自备试剂，详情见网站说明书 |
| 中文名称 | 羟脯氨酸(HYP)含量检测试剂盒 |
| 英文名称 | Hydroxyproline(HYP) Content Assay Kit |
| 检测方法 | 微量法 Spectrophotometer/Microplate Reader |
| 储存条件 | Store at 2-8℃，6 months |
| 规格 | 100T/96S |
| 单位 | 盒 |
测定意义：HYP是机体胶原蛋白主要成分之一，胶原蛋白大多分布于皮肤、腱、软骨和血管等，因此HYP含量是反映胶原组织代谢及纤维化程度的一项重要指标。
测定原理：样品经水解产生游离的HYP，进一步被氯胺T氧化，氧化产物与对二甲氨基苯甲醛反应，产生红色化合物，在560nm处有特征吸收峰。通过测定样品水解液560nm吸光值，可计算HYP含量。
需要的仪器，耗材:天平、烘箱、玻璃管、离心机、水浴锅、可见分光光度计/酶标仪、微量石英比色皿/96孔板、6mol/L盐酸和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
Standard Curve for Hydroxyproline(HYP) Content Assay Kit
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Liu Xiaoyun, Deng Yuping, Liang Zeyu, Qiao Dan, Zhang Wentian, Wang Mian, Li Feifei, Liu Jiannan, Wu Yaobing, Chen Guangxin, Liu Yan, Tan Wenchang, Xing Jian, Huang Wenhua, Zhao Dongliang, Li Yanbing
IF：
5.7000
Publish_to：
Frontiers in Bioengineering and Biotechnology
PMID：
38694621
The roles of 10-methacryloyloxydecyl dihydrogen phosphate and its calcium salt in preserving the adhesive–dentin hybrid layer
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Author：
Xin Jin, Fei Han, Qi Wang, Xiaojun Yuan, Qiyue Zhou, Haifeng Xie, Lina Niu, Chen Chen
IF：
5.6870
Publish_to：
DENTAL MATERIALS
PMID：
35715248
Targeting Endothelial Necroptosis Disrupts Profibrotic Endothelial–Hepatic Stellate Cells Crosstalk to Alleviate Liver Fibrosis in Nonalcoholic Steatohepatitis
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Author：
Mengli Yan, Hui Li, Shiyu Xu, Jinyan Wu, Jiachen Li, Chengju Xiao, Chunheng Mo, Bi-Sen Ding
IF：
5.6000
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
37511074
Endothelial anthrax toxin receptor 2 plays a protective role in liver fibrosis
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Author：
Xiaojuan Huang, Liyin Zhang, Wei Luo, Yu Zeng, Xiaoxue Li, Nan Yang, Wenwen Huang, Bi-Sen Ding
IF：
5.5000
Publish_to：
Frontiers in Cell and Developmental Biology
PMID：
38322497
Integrating transcriptomics and network pharmacology to reveal the mechanisms of total Rhizoma Coptidis alkaloids against nonalcoholic steatohepatitis
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Author：
Juan Li, Zhengcai Ma, Zhipeng Yang, Maochun Yang, Changsheng Li, Mengmeng Li, Xiaoduo Li, Xiantao Chen, Ma Hang, Wanqun Chen, Xiaoli Ye, Xuegang Li
IF：
5.4000
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
38103844
Polyphenol-Enriched Extract of Lacquer Sap Used as a Dentine Primer with Benefits of Improving Collagen Cross-Linking and Antibacterial Functions
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Author：
Ying Zhao, Xi He, Han Wang, Huimin Wang, Zuosen Shi, Song Zhu, Zhanchen Cui
IF：
5.3950
Publish_to：
ACS Biomaterials Science & Engineering
PMID：
35793160
Synergistic effects of graphene quantum dots and carbodiimide in promoting resin–dentin bond durability
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Author：
Wendy Chen, Huimin Jin, Heng Zhang, Leping Wu, Guoqing Chen, Hui Shao, Shengrui Wang, Xiaoxue He, Shunli Zheng, Chris Ying Cao, Quan-Li Li
IF：
5.3040
Publish_to：
DENTAL MATERIALS
PMID：
34465445
Biejiajian pill inhibits progression of hepatocellular carcinoma by downregulating PDGFRβ signaling in cancer-associated fibroblasts
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Author：
Weicong Chen, Xuemei Yang, Jialing Sun, Yuyao Chen, Wenting Zhao, Chunyu He, Haiyan An, Jie Pang, Wei Xu, Bin Wen, Haitao Sun, Songqi He
IF：
5.1950
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
36240978
Discovery of LH10, a novel fexaramine-based FXR agonist for the treatment of liver disease
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Author：
Wanqiu Huang, Zhijun Cao, Wenxin Wang, Zhongcheng Yang, Shixuan Jiao, Ya Chen, Siliang Chen, Luyong Zhang, Zheng Li
IF：
5.1000
Publish_to：
BIOORGANIC CHEMISTRY
PMID：
38199141
Interactions of two phosphate ester monomers with hydroxyapatite and collagen fibers and their contributions to dentine bond performance
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Author：
Fei Han, Xin Jin, Xiaojun Yuan, Zehua Bai, Qi Wang, Haifeng Xie
IF：
4.9910
Publish_to：
JOURNAL OF DENTISTRY
PMID：
35550398
MicroSPECT Imaging-Guided Treatment of Idiopathic Pulmonary Fibrosis in Mice with a Vimentin-Targeting 99mTc-Labeled N-Acetylglucosamine-Polyethyleneimine
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Author：
Deliang Zhang, Rongqiang Zhuang, Jindian Li, Yuting Lv, Xia Yang, Weimin Pan, Xianzhong Zhang
IF：
4.9390
Publish_to：
MOLECULAR PHARMACEUTICS
PMID：
34657437
Resveratrol Ameliorates Fibrosis in Rheumatoid Arthritis-Associated Interstitial Lung Disease via the Autophagy–Lysosome Pathway
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Author：
Lanxin Bao, Jing Ye, Nannan Liu, Yubao Shao, Wenhao Li, Xuefei Fan, Dahai Zhao, Hongzhi Wang, Xiaoyu Chen
IF：
4.9270
Publish_to：
MOLECULES
PMID：
36500562
Gynostemma Pentaphyllum ameliorates CCl4-induced liver injury via PDK1/Bcl-2 pathway with comprehensive analysis of network pharmacology and transcriptomics
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Author：
Linlan Hu, Xin Zhao, Xian He, Yafei Guo, Hanxiao Cheng, Shaoting Chen, Guangde Zhou, Jiabo Wang, Yawen Lu
IF：
4.9000
Publish_to：
Chinese Medicine
PMID：
38750545
Rapid formation of 3D: Decellularized extracellular matrix spheroids for enhancing bone formation
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Author：
Yanmin Zhang, Yuting Feng, Qin Shao, Zhiwei Jiang, Guoli Yang
IF：
4.8540
Publish_to：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
PMID：
36355784
Exploring TβRI inhibitors from Arenaria kansuensis based on 3D-QSAR, molecular docking and molecular dynamics simulation methods and its anti-pulmonary fibrosis molecular mechanism validation
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Author：
Na Shen, Ziyao Shao, Huawei Xin, Fengyuan Che, Yulei Cui
IF：
4.8000
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
39245240
Purification and Identification of Novel Dipeptidyl Peptidase IV Inhibitory Peptides Derived from Bighead Carp (Hypophthalmichthys nobilis)
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Author：
Hanzhi Zheng, Leyan Zhao, Yushuo Xie, Yuqing Tan
IF：
4.7000
Publish_to：
Foods
PMID：
39272410
Extrafibrillar demineralization: Yes or no?
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Author：
Qingli Zhou, Yuzhu Li, Ting Xiao, Qi Zhong, Leping Wu, Zheng Zhou, Haiming Wong, Quan-Li Li
IF：
4.6000
Publish_to：
DENTAL MATERIALS
PMID：
38821839
ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice
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Author：
Ming Li, Zhifeng Yang, Zhaoyuan Song, Cunxiang Bo, Shuo Wang, Qiang Jia
IF：
4.5710
Publish_to：
TOXICOLOGY
PMID：
36526012
Integrated analysis and validation of TRIM23/p53 signaling pathway in hepatic stellate cells ferroptosis and liver fibrosis
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Author：
Jie Chen, Rui Zhang, Feng Li, Shengli Lin, Jian Wang
IF：
4.5000
Publish_to：
DIGESTIVE AND LIVER DISEASE
PMID：
37495427
Qizhu Rougan Granules suppress liver fibrosis by inhibiting the expression of the P2Y14 receptor on hepatic stellate cells
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Author：
Yujing Tao, Qun Niu, Yuanqian Yao, Kaixin Wang, Haijian Dong, Xin Zhao, Zijian Zeng, Hui Li
IF：
4.4000
Publish_to：
Frontiers in Pharmacology
PMID：
39850561
Tectona grandis leaf extract ameliorates hepatic fibrosis: Modulation of TGF- β /Smad signaling pathway and upregulating MMP3/TIMP1 ratio
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Author：
Somayya Tariq, Brice Landry Koloko, Ayesha Malik, Sidra Rehman, Bushra Ijaz, Ahmad Ali Shahid
IF：
4.3600
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
33610708
Near-infrared Nrf2 activator IR-61 dye alleviates radiation-induced lung injury
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Author：
Jiancheng Zheng, Yang Wang, Ziwen Wang, Wanchao Chen, Min Luo, Can Zhang, Yawei Wang, Long Chen, Feng Wu, Wei Yang, Zeyu Yang, Yu Wang, Chunmeng Shi
IF：
4.3540
Publish_to：
FREE RADICAL RESEARCH
PMID：
36201846
Arctigenin induces activated HSCs quiescence via AMPK-PPARγ pathway to ameliorate liver fibrosis in mice
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Author：
Mengjia Lv, Shiyi Chen, Mengwen Shan, Yuan Si, Chenggang Huang, Jing Chen, Likun Gong
IF：
4.2000
Publish_to：
EUROPEAN JOURNAL OF PHARMACOLOGY
PMID：
38679116
Macromolecular crowding enhances matrix proteins deposition in tissue engineered vascular grafts
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Author：
Qing Liu, Jiang Liu, Xu Heng Sun, Jian Yi Xu, Cong Xiao, Hong Jin Jiang, Yin Di Wu, Zhanyi Lin
IF：
4.1000
Publish_to：
Tissue Engineering Part A
PMID：
38318797
Elevated retinal fibrosis in experimental myopia is involved in the activation of the PI3K/AKT/ERK signaling pathway
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Author：
Bo Bao, Jinpeng Liu, Tuling Li, Zhaohui Yang, Guimin Wang, Jizhao Xin, Hongsheng Bi, Dadong Guo
IF：
3.9000
Publish_to：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
PMID：
37290701
Integrated strategy of RNA-sequencing and network pharmacology for exploring the protective mechanism of Shen-Shi-Jiang-Zhuo formula in rat with non-alcoholic fatty liver disease
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Author：
Zheng Xu, Fan-Wei Wu, Xuan Niu, Xiao-Peng Lu, Yan-Rong Li, Shu-Ting Zhang, Jun-Zhao Ou, Xue-Mei Wang
IF：
3.8890
Publish_to：
PHARMACEUTICAL BIOLOGY
PMID：
36124995
Comparative Analysis of Hyaline Cartilage Characteristics and Chondrocyte Potential for Articular Cartilage Repair
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Author：
Chiu Cheng, Zheng Kaiwen, Xue Mengxin, Du Dajiang
IF：
3.8000
Publish_to：
ANNALS OF BIOMEDICAL ENGINEERING
PMID：
38190025
Plunge-Freezing Cryopreservation of Tendons
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Author：
Haoyue Li, Xinmeng Liu, Liming Zhang, Lei Zhang
IF：
3.7000
Publish_to：
LANGMUIR
PMID：
38916446
Transcriptome profile analysis reveals a silica-induced immune response and T fibrosis in a silicosis rat model
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Author：
赵宏宇
IF：
3.6000
Publish_to：
PMID：
32721576
Silica nanoparticle induces pulmonary fibroblast transdifferentiation via macrophage route: Potential mechanism revealed by proteomic analysis
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Author：
Ning Li, Luyao Wang, Fan Shi, Pan Yang, Kun Sun, Jing Zhang, Xiaojing Yang, Xiaoming Li, Fuhai Shen, Heliang Liu, Yulan Jin, Sanqiao Yao
IF：
3.5000
Publish_to：
TOXICOLOGY IN VITRO
PMID：
34271082
Enhanced osteogenicity of the demineralized bone-dermal matrix composite by the optimal partial demineralization for sustained release of bioactive molecules
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Author：
Tong Ma, Dangli Ren, Jingjing Wang, Feng Fu, Wendell Q. Sun, Hongtao Sun
IF：
3.4000
Publish_to：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
PMID：
38247243
Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs
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Author：
Wei Shuai, Wang Wei, Li Li, Meng Hao-Ye, Feng Chun-Zhen, Dong Yu-Ying, Fang Xi-Chi, Dong Qi-Qiang, Jiang Wen, Xin Hai-Li, Li Zhan-Zhen, Wang Xin
IF：
3.3290
Publish_to：
Military Medical Research
PMID：
33685528
miR-141-3p attenuates inflammation and oxidative stress–induced pulmonary fibrosis in ARDS via the Keap1/Nrf2/ARE signaling pathway
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Author：
Long Guangwen, Zhang Qian, Yang Xiulin, Sun Hongpeng, Ji Chunling
IF：
3.3000
Publish_to：
IMMUNOLOGIC RESEARCH
PMID：
38865000
Effect of Qiling Jiaogulan Powder on Pulmonary Fibrosis and Pulmonary Arteriole Remodeling in Low-Temperature-Exposed Broilers
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Author：
Juan Yu, Peng Li, Zhibian Duan, Xingyou Liu
IF：
3.2310
Publish_to：
Animals
PMID：
36611616
Characterization, expression profiling and functional characterization of cathepsin Z (CTSZ) in turbot (Scophthalmus maximus L.)
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Author：
XinCaiChengbinGaoHuanhuanSongNingYangQiangFuFenghu
IF：
3.1850
Publish_to：
Fish & Shellfish Immunology Pages:599-608
PMID：
30359754
USP33 promotes nonalcoholic fatty acid disease-associated fibrosis in gerbils via the c-myc signaling
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Author：
Xianfu Ke, Huiying Hu, Qianyu Peng, Huazhong Ying, Xiaofeng Chu
IF：
3.1000
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
37267862
miR-15a-5p regulates myocardial fibrosis in atrial fibrillation by targeting Smad7
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Author：
Dan He, Zhong-bao Ruan, Gui-xian Song, Ge-cai Chen, Fei Wang, Mei-xiang Wang, Mao-kun Yuan, Li Zhu
IF：
2.9840
Publish_to：
PeerJ
PMID：
35036160
Fluorofenidone attenuates paraquat‑induced pulmonary fibrosis by regulating the PI3K/Akt/mTOR signaling pathway and autophagy
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Author：
Feiya Jiang, Sha Li, Yu Jiang, Zhuo Chen, Tongtong Wang, Wen Liu
IF：
2.9520
Publish_to：
Molecular Medicine Reports
PMID：
33786626
Potential cardioprotective effect of paroxetine against ventricular remodeling in an animal model of myocardial infarction: a comparative study
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Author：
Alonazi Asma S., Almodawah Sara, Aldigi Rana, Bin Dayel Anfal, Alamin Maha, Almotairi Ahmad R., El-Tohamy Maha F., Alharbi Hana, Ali Rehab, Alshammari Tahani K., Alrasheed Nouf M.
IF：
2.8000
Publish_to：
BMC Pharmacology & Toxicology
PMID：
39696491
Cepharanthine attenuates pulmonary fibrosis via modulating macrophage M2 polarization
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Author：
Bao Jiaqi, Liu Chang, Song Huafeng, Mao Zheying, Qu Wenxin, Yu Fei, Shen Yifei, Jiang Jingjing, Chen Xiao, Wang Ruonan, Wang Qi, Chen Weizhen, Zheng Shufa, Chen Yu
IF：
2.6000
Publish_to：
BMC Pulmonary Medicine
PMID：
39261812
T1/T2 mapping as a non-invasive method for evaluating liver fibrosis based on correlation of biomarkers: a preclinical study
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Author：
Shuqin Xue, Yujie Zhu, Min Shao, Kun Zhu, Jing Rong, Tongtong Liu, Xiujuan Yin, Saisai Zhang, Likang Yin, Xiao Wang
IF：
2.5000
Publish_to：
BMC GASTROENTEROLOGY
PMID：
40016673
Effect of glabridin combined with bakuchiol on UVB-induced skin damage and its underlying mechanism: An experimental study
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Author：
Guanjie Peng, Yangsi Li, Yiyan Zeng, Bowen Sun, Lanyue Zhang, Qingqing Liu
IF：
2.3000
Publish_to：
Journal of Cosmetic Dermatology
PMID：
38497297
Angiosome-Guided Perfusion Decellularization of Fasciocutaneous Flaps
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Author：
Liya Yang, Xueshan Bai, Yuanbo Liu, Shanshan Li, Zixiang Chen, Tinglu Han, Shenyang Jin, Tingjun Xie, Danying Wang, Shuai Yue, Miao Wang, Shan Zhu, Mengqing Zang
IF：
2.2000
Publish_to：
JOURNAL OF RECONSTRUCTIVE MICROSURGERY
PMID：
39191422
Effects of demineralization mode and particle size of allogeneic bone powder on its physical and chemical properties
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Author：
Song Kun-xiu, Ji Shao-lin, Zhao Yong-jie, Zhang Hao-ran, Ma Rong-xing, Zhang Jing-yu, Hu Yong-cheng
IF：
1.7520
Publish_to：
CELL AND TISSUE BANKING
PMID：
35831637
Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy
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Author：
Shi Sui, Yang Hou
IF：
1.5890
Publish_to：
SCANDINAVIAN CARDIOVASCULAR JOURNAL
PMID：
34296634
Repair of tendons treated with peracetic acid–ethanol and gamma irradiation by EDC combined with NHS: a morphological, biochemical and biomechanical study in vitro
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Author：
Ma Rong-xing, Li Rui-feng, Deng Xiao-qiang, Qiao Rui-qi, Li Ji-kai, Song Kun-xiu, Ji Shao-lin, Hu Yong-cheng
IF：
1.5000
Publish_to：
CELL AND TISSUE BANKING
PMID：
36797536
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