中文
英语
产品简介
| 自备试剂 | 该试剂盒实验过程中需自备试剂，详情见网站说明书 |
| 中文名称 | 脯氨酸（Pro）含量检测试剂盒 |
| 英文名称 | Proline (Pro) Content Assay Kit |
| 检测方法 | 微量法 |
| 储存条件 | 2-8℃保存6个月 |
| 规格 | 100T/96S 200T/192S |
| 单位 | 盒 |
测定意义：Pro广泛存在于动物、植物、微生物和培养细胞中，逆境条件下，植物体内Pro含量显著增加。Pro增加量在一定程度上反映了抗逆性，抗旱性强的品种往往积累较多的脯氨酸。因此，脯氨酸增加量可以作为抗逆育种的生理指标之一。
测定原理：用磺基水杨酸（SA）提取Pro，加热处理后，Pro与酸性茚三酮溶液反应生成红色；加甲苯萃取后，在520nm测定吸光度。
需要的仪器，耗材:可见分光光度计/酶标仪、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、冰乙酸25mL、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
Standard Curve for Proline(PRO) Content Assay Kit
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IF：
3.7520
Publish_to：
PLoS One
PMID：
35709203
Ursonic acid from medicinal herbs inhibits PRRSV replication through activation of the innate immune response by targeting the phosphatase PTPN1
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Author：
Yuanqi Yang, Yanni Gao, Haifeng Sun, Juan Bai, Jie Zhang, Lujie Zhang, Xing Liu, Yangyang Sun, Ping Jiang
IF：
3.7000
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VETERINARY RESEARCH
PMID：
38783392
The MADS transcription factor GhFYF is involved in abiotic stress responses in upland cotton (Gossypium hirsutum L.)
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Author：
Yujun Xue, Liang Ma, Hantao Wang, Pengbo Hao, Shuaishuai Cheng, Zhengzheng Su, Lin Li, Shuxun Yu, Hengling Wei
IF：
3.6880
Publish_to：
GENE
PMID：
34979233
Molecular identification and expression patterns of sweet cherry HIPPs and functional analysis of PavHIPP16 in cold stress
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Author：
Yu Runrun, Hou Qiandong, Deng Hong, Xiao Ling, Liu Ke, Wu Yawei, Qiao Guang
IF：
3.6000
Publish_to：
PLANTA
PMID：
39505755
Dioscorea composita WRKY3 positively regulates salt-stress tolerance in transgenic Arabidopsis thaliana
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Author：
Shangjie Yu, Xin Lan, Jianchan Zhou, Kaixiang Gao, Chunmei Zhong, Jun Xie
IF：
3.5490
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JOURNAL OF PLANT PHYSIOLOGY
PMID：
34923363
Integrative Physiological and Transcriptome Analysis Reveals the Mechanism of Cd Tolerance in Sinapis alba
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Author：
Mengxian Cai, Tinghai Yang, Shiting Fang, Lvlan Ye, Lei Gu, Hongcheng Wang, Xuye Du, Bin Zhu, Tuo Zeng, Tao Peng
IF：
3.5000
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Genes
PMID：
38137046
Comparative transcriptome analysis of leaves during early stages of chilling stress in two different chilling-tolerant brown-fiber cotton cultivars
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Author：
Shouwu Tang, Yajie Xian, Fei Wang, Cheng Luo, Wu Song, Shuangquan Xie, Xifeng Chen, Aiping Cao, Hongbin Li, Haifeng Liu
IF：
3.2400
Publish_to：
PLoS One
PMID：
33561168
Genome-Wide Identification and Expression Divergence of CBF Family in Actinidia arguta and Functional Analysis of AaCBF4 Under Cold Stress
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Author：
Sumei Li, Qina Zhang, Zhenzhen Zhang, Peng Zhang, Congcong Li, Leiming Sun, Jinbao Fang, Ran Wang, Feng Wei, Yukuo Li, Miaomiao Lin, Xiujuan Qi
IF：
3.2000
Publish_to：
Life-Basel
PMID：
40003636
A Cloned Gene HuBADH from Hylocereus undatus Enhanced Salt Stress Tolerance in Transgenic Arabidopsis thaliana Plants
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Author：
Yujie Qu, Zhan Bian, Jaime A. Teixeira da Silva, Quandong Nong, Wenran Qu, Guohua Ma
IF：
3.1000
Publish_to：
Frontiers in Bioscience-Landmark
PMID：
37114532
B-box containing protein 1 from Malus domestica (MdBBX1) is involved in the abiotic stress response
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Author：
Yaqing Dai, Ying Lu, Zhou Zhou, Xiaoyun Wang, Hongjuan Ge, Qinghua Sun
IF：
3.0610
Publish_to：
PeerJ
PMID：
35178298
Potential Involvement of MnCYP710A11 in Botrytis cinerea Resistance in Arabidopsis thaliana and Morus notabilis
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Author：
Hui An, Donghao Wang, Lin Yu, Hongshun Wu, Yue Qin, Shihao Zhang, Xianling Ji, Youchao Xin, Xiaodong Li
IF：
2.8000
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Genes
PMID：
39062632
Exogenous melatonin confers cold tolerance in rapeseed (Brassica napus L.) seedlings by improving antioxidants and genes expression
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Author：
Yan Lei, Huang He, Ali Raza, Zeng Liu, Ding Xiaoyu, Wang Guijuan, Lv Yan, Cheng Yong, Zou Xiling
IF：
2.7340
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Plant Signaling & Behavior
PMID：
36205498
Evaluation of waterlogging tolerance and responses of protective enzymes to waterlogging stress in pumpkin
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Author：
Zhenwei Liu, Dandan Qiao, Zhenyu Liu, Pengwei Wang, Li Sun, Xinzheng Li
IF：
2.7000
Publish_to：
PeerJ
PMID：
37101787
Multiple Perspectives of Study on the Potential of Bacillus amyloliquefaciens JB20221020 for Alleviating Nutrient Stress in Lettuce
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Author：
Bai Yinshuang, Zheng Xianqing, Ma Juan, Liu Hua, Zeng Haijuan, Zhang Fujian, Wang Jinbin, Song Ke
IF：
2.3000
Publish_to：
CURRENT MICROBIOLOGY
PMID：
38890167
Effects of drought stress on seed germination and early seeding growth in Ferula ferulaeoides (Steud.) Korov.
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Author：
Ma Ying, Liao Kai, Zhu Yun, Lu Xin, Wang Kuan, Zhang Xue Rui
IF：
1.6000
Publish_to：
Brazilian Journal of Botany
PMID：
技术问答
（1）
注意：
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你们这个组织需要称取0.1g,是植物哪个部位的组织？，叶片，茎秆，还是根系，组织是新鲜样品，还是烘干之后的？不是很清楚?2023-06-20 11:09:34
您好，试剂盒没有具体的组织部位要求，完全取决于您实验的要求。是否烘干也取决您需要于按鲜重质量还是干重质量计算含量。