中文
英语
产品简介
| 自备试剂 | 该试剂盒实验过程中需自备试剂，详情见网站说明书 |
| 中文名称 | 脯氨酸（Pro）含量检测试剂盒 |
| 英文名称 | Proline (Pro) Content Assay Kit |
| 检测方法 | 微量法 |
| 储存条件 | 2-8℃保存6个月 |
| 运输条件 | 冷藏运输 |
| 规格 | 100T/96S 200T/192S |
| 单位 | 盒 |
测定意义：Pro 广泛存在于动物、植物、微生物和培养细胞中，逆境条件下，植物体内Pro 含量显著增加。Pro 增加量在一定程度上反映了抗逆性，抗旱性强的品种往往积累较多的脯氨酸。因此，脯氨酸增加量可以作为抗逆育种的生理指标之一。
测定原理：用磺基水杨酸（SA）提取Pro，加热处理后，Pro 与酸性茚三酮溶液反应生成红色，在520nm 测定吸光度。
需自备的仪器和用品：可见分光光度计、水浴锅、台式离心机、可调式移液器、1 mL 玻璃比色皿、冰乙酸50mL、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
Standard Curve for Proline(PRO) Content Assay Kit
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Yanyan Yu, Yong Ni, Tian Qiao, Xiaomin Ji, Jinghao Xu, Bo Li, Qinghua Sun
IF：
3.7520
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PLoS One
PMID：
35709203
Ursonic acid from medicinal herbs inhibits PRRSV replication through activation of the innate immune response by targeting the phosphatase PTPN1
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Yuanqi Yang, Yanni Gao, Haifeng Sun, Juan Bai, Jie Zhang, Lujie Zhang, Xing Liu, Yangyang Sun, Ping Jiang
IF：
3.7000
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VETERINARY RESEARCH
PMID：
38783392
The MADS transcription factor GhFYF is involved in abiotic stress responses in upland cotton (Gossypium hirsutum L.)
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Yujun Xue, Liang Ma, Hantao Wang, Pengbo Hao, Shuaishuai Cheng, Zhengzheng Su, Lin Li, Shuxun Yu, Hengling Wei
IF：
3.6880
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GENE
PMID：
34979233
Molecular identification and expression patterns of sweet cherry HIPPs and functional analysis of PavHIPP16 in cold stress
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Author：
Yu Runrun, Hou Qiandong, Deng Hong, Xiao Ling, Liu Ke, Wu Yawei, Qiao Guang
IF：
3.6000
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PLANTA
PMID：
39505755
Dioscorea composita WRKY3 positively regulates salt-stress tolerance in transgenic Arabidopsis thaliana
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Author：
Shangjie Yu, Xin Lan, Jianchan Zhou, Kaixiang Gao, Chunmei Zhong, Jun Xie
IF：
3.5490
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JOURNAL OF PLANT PHYSIOLOGY
PMID：
34923363
Integrative Physiological and Transcriptome Analysis Reveals the Mechanism of Cd Tolerance in Sinapis alba
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Mengxian Cai, Tinghai Yang, Shiting Fang, Lvlan Ye, Lei Gu, Hongcheng Wang, Xuye Du, Bin Zhu, Tuo Zeng, Tao Peng
IF：
3.5000
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Genes
PMID：
38137046
Comparative transcriptome analysis of leaves during early stages of chilling stress in two different chilling-tolerant brown-fiber cotton cultivars
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Shouwu Tang, Yajie Xian, Fei Wang, Cheng Luo, Wu Song, Shuangquan Xie, Xifeng Chen, Aiping Cao, Hongbin Li, Haifeng Liu
IF：
3.2400
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PLoS One
PMID：
33561168
Genome-Wide Identification and Expression Divergence of CBF Family in Actinidia arguta and Functional Analysis of AaCBF4 Under Cold Stress
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Author：
Sumei Li, Qina Zhang, Zhenzhen Zhang, Peng Zhang, Congcong Li, Leiming Sun, Jinbao Fang, Ran Wang, Feng Wei, Yukuo Li, Miaomiao Lin, Xiujuan Qi
IF：
3.2000
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Life-Basel
PMID：
40003636
A Cloned Gene HuBADH from Hylocereus undatus Enhanced Salt Stress Tolerance in Transgenic Arabidopsis thaliana Plants
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Author：
Yujie Qu, Zhan Bian, Jaime A. Teixeira da Silva, Quandong Nong, Wenran Qu, Guohua Ma
IF：
3.1000
Publish_to：
Frontiers in Bioscience-Landmark
PMID：
37114532
B-box containing protein 1 from Malus domestica (MdBBX1) is involved in the abiotic stress response
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Author：
Yaqing Dai, Ying Lu, Zhou Zhou, Xiaoyun Wang, Hongjuan Ge, Qinghua Sun
IF：
3.0610
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PeerJ
PMID：
35178298
Potential Involvement of MnCYP710A11 in Botrytis cinerea Resistance in Arabidopsis thaliana and Morus notabilis
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Author：
Hui An, Donghao Wang, Lin Yu, Hongshun Wu, Yue Qin, Shihao Zhang, Xianling Ji, Youchao Xin, Xiaodong Li
IF：
2.8000
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Genes
PMID：
39062632
Exogenous melatonin confers cold tolerance in rapeseed (Brassica napus L.) seedlings by improving antioxidants and genes expression
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Author：
Yan Lei, Huang He, Ali Raza, Zeng Liu, Ding Xiaoyu, Wang Guijuan, Lv Yan, Cheng Yong, Zou Xiling
IF：
2.7340
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Plant Signaling & Behavior
PMID：
36205498
Evaluation of waterlogging tolerance and responses of protective enzymes to waterlogging stress in pumpkin
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Author：
Zhenwei Liu, Dandan Qiao, Zhenyu Liu, Pengwei Wang, Li Sun, Xinzheng Li
IF：
2.7000
Publish_to：
PeerJ
PMID：
37101787
Multiple Perspectives of Study on the Potential of Bacillus amyloliquefaciens JB20221020 for Alleviating Nutrient Stress in Lettuce
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Author：
Bai Yinshuang, Zheng Xianqing, Ma Juan, Liu Hua, Zeng Haijuan, Zhang Fujian, Wang Jinbin, Song Ke
IF：
2.3000
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CURRENT MICROBIOLOGY
PMID：
38890167
Effects of drought stress on seed germination and early seeding growth in Ferula ferulaeoides (Steud.) Korov.
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Author：
Ma Ying, Liao Kai, Zhu Yun, Lu Xin, Wang Kuan, Zhang Xue Rui
IF：
1.6000
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Brazilian Journal of Botany
PMID：
技术问答
（1）
注意：
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你们这个组织需要称取0.1g,是植物哪个部位的组织？，叶片，茎秆，还是根系，组织是新鲜样品，还是烘干之后的？不是很清楚?2023-06-20 11:09:34
您好，试剂盒没有具体的组织部位要求，完全取决于您实验的要求。是否烘干也取决您需要于按鲜重质量还是干重质量计算含量。