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英语
谷丙转氨酶(GPT/ALT)活性检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:Glutamic-pyruvic Transaminase(GPT) Activity Assay Kit
储存条件:Store at 2-8℃，6 months
产品简介
| 中文名称 | 谷丙转氨酶(GPT/ALT)活性检测试剂盒 |
| 英文名称 | Glutamic-pyruvic Transaminase(GPT) Activity Assay Kit |
| 检测方法 | 可见分光光度法 Spectrophotometer |
| 储存条件 | Store at 2-8℃，6 months |
| 规格 | 50T/24S |
| 单位 | 盒 |
测定意义：GPT（EC 2.6.1.2）广泛存在于动物、植物、微生物和培养细胞中，催化氨基酸和酮酸转氨基反应，在氨基酸代谢中具有重要作用。此外，哺乳动物肝细胞GPT 活性很高，当肝细胞坏死，GPT 释放到血液中，血清GPT 活性显著增高。因此，GPT 被世界卫生组织推荐为肝功能损害最敏感的检测指标。
测定原理：GPT 催化丙氨酸和α-酮戊二酸发生转氨基反应，生成丙酮酸和谷氨酸；加入2,4-二硝基苯肼溶液，不仅终止上述反应，而且与酮酸中的羰基加成，生成丙酮酸苯腙；苯腙在碱性条件下呈红棕色，可以在505nm 读取吸光值并计算酶活力。
需自备的仪器和用品：可见分光光度计、水浴锅、台式离心机、可调式移液器、1 mL 玻璃比色皿、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Food & Function
PMID：
34846398
Potential antioxidative components from Syringa oblata Lindl stems revealed by affinity ultrafiltration with multiple drug targets
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Author：
Zhiqiang Li, Haonan Zhang, Wanting Li, Min Yao, Huimin Yu, Mingzhen He, Yulin Feng, Zhifeng Li
IF：
5.3070
Publish_to：
BIOORGANIC CHEMISTRY
PMID：
37178648
Gynostemma Pentaphyllum ameliorates CCl4-induced liver injury via PDK1/Bcl-2 pathway with comprehensive analysis of network pharmacology and transcriptomics
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Author：
Linlan Hu, Xin Zhao, Xian He, Yafei Guo, Hanxiao Cheng, Shaoting Chen, Guangde Zhou, Jiabo Wang, Yawen Lu
IF：
5.3000
Publish_to：
Chinese Medicine
PMID：
38750545
Lactoferrin Alleviates Acute Alcoholic Liver Injury by Improving Redox-Stress Response Capacity in Female C57BL/6J Mice
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Author：
Deming Li, Zhiqiang Hu, Qian He, Yaxin Guo, Yu Chong, Jiaying Xu, Liqiang Qin
IF：
5.2790
Publish_to：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
34873911
A glucose-mediated antibiotic resistance metabolic flux from glycolysis, the pyruvate cycle, and glutamate metabolism to purine metabolism
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Author：
Jiao Xiang, Shi-wen Wang, Yuan Tao, Jing-zhou Ye, Ying Liang, Xuan-xian Peng, Li-fen Yang, Hui Li
IF：
5.2000
Publish_to：
Frontiers in Microbiology
PMID：
37915850
Pyrroloquinoline quinone ameliorates liver injury in mice induced by cyclophosphamide
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Author：
Qian Li, Yang Fei, Lin Xinhui, Jiang Su, Zhang Yun, Tang Yunping
IF：
5.1900
Publish_to：
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
PMID：
34997497
Design,synthesis,and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
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Author：
Zhongcheng Yang, Zhijun Cao, Wenxin Wang, Ya Chen, Wanqiu Huang, Shixuan Jiao, Siliang Chen, Lianru Chen, Yuxia Liu, Jianming Mao, Luyong Zhang, Zheng Li
IF：
5.1000
Publish_to：
BIOORGANIC CHEMISTRY
PMID：
37300962
Hepatoprotective effects of peach gum polysaccharides against alcoholic liver injury: moderation of oxidative stress and promotion of lipid metabolism
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Author：
Bingjie Zhou, Pinpin Liu, Xiangao Yao, Huijie Cao, Hang Zhu, Qiao Wang, Yan Liu, Min Fang, Yongning Wu, Zhiyong Gong
IF：
5.0000
Publish_to：
Frontiers in Nutrition
PMID：
38283909
Effect of Dietary Crude Protein and Apparent Metabolizable Energy Levels on Growth Performance, Nitrogen Utilization, Serum Parameter, Protein Synthesis, and Amino Acid Metabolism of 1- to 10-Day-Old Male Broilers
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Author：
Yao Yu, Chunxiao Ai, Caiwei Luo, Jianmin Yuan
IF：
4.9000
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
39000537
Scutellarin inhibits inflammatory PANoptosis by diminishing mitochondrial ROS generation and blocking PANoptosome formation
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Author：
Tao Yuan, Hai-Yan Yang, Ya-Ping Li, Zi-Jian Shi, Zhi-Ya Zhou, Yi-Ping You, Hua-Yu Ke, Liang Yan, Li-Hui Xu, Dong-Yun Ouyang, Xian-Hui He, Qing-Bing Zha
IF：
4.8000
Publish_to：
INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：
39029229
Parabacteroides distasonis-Derived Outer Membrane Vesicles Enhance Antitumor Immunity Against Colon Tumors by Modulating CXCL10 and CD8+ T Cells
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Author：
Rongyao Liang, Pei Li, Na Yang, Xiaoyi Xiao, Jing Gong, Xingyuan Zhang, Yunuan Bai, Yanlong Chen, Zhiyong Xie, Qiongfeng Liao
IF：
4.7000
Publish_to：
Drug Design Development and Therapy
PMID：
38828018
Study on Dihydromyricetin Improving Aflatoxin Induced Liver Injury Based on Network Pharmacology and Molecular Docking
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Author：
Xiaoying Zhu, Silu Liu, Hongyan Pei, Weijia Chen, Ying Zong, Yan Zhao, Jianming Li, Rui Du, Zhongmei He
IF：
4.6000
Publish_to：
Toxics
PMID：
37755770
Transcriptomics and metabolomics together reveal the underlying mechanism of heroin hepatotoxicity
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Author：
Yingbiao Yue, Lei Zou, Jie Tao, Lin Yin, Zhenrong Xie, Yu Xia, Zunyue Zhang, Kunhua Wang, Mei Zhu
IF：
4.5710
Publish_to：
TOXICOLOGY
PMID：
36502556
Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp
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Author：
Wen-bin Liu, Min-meng Wang, Liu-ye Dai, Sheng-hua Dong, Xiu-dan Yuan, Shu-li Yuan, Yi Tang, Jin-hui Liu, Liang-yue Peng, Ya-mei Xiao
IF：
4.5660
Publish_to：
Frontiers in Physiology
PMID：
34149447
Pyrogallol promotes growth arrest by activating the p53-mediated up-regulation of p21 and p62/SQSTM1-dependent degradation of β-catenin in nonsmall cell lung cancer cells
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Author：
Beixian Zhou, Linxin Wang, Zhixian Ren, Yueyun Liang, Sushan Yang, Yuehan Zhang, Siyao Che, Weiyi Fang
IF：
4.5000
Publish_to：
ENVIRONMENTAL TOXICOLOGY
PMID：
38108618
Non-absorbable antibiotic treatment inhibits colorectal cancer liver metastasis by modulating deoxycholic acid metabolism by intestinal microbes
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Author：
Junjie Deng, Wei Yuan, Qin Tan, Xundong Wei, Jie Ma
IF：
4.4780
Publish_to：
Journal of Cancer
PMID：
35154445
L-arginine combination with 5-fluorouracil inhibit hepatocellular carcinoma cells through suppressing iNOS/NO/AKT-mediated glycolysis
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Author：
Hu Yile, Xing Yihao, Fan Gaolu, Xie Huaxia, Zhao Qingzan, Liu Ling
IF：
4.4000
Publish_to：
Frontiers in Pharmacology
PMID：
38841361
Rhubarb granule promotes diethylnitrosamine-induced liver tumorigenesis by activating the oxidative branch of pentose phosphate pathway via G6PD in rats
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Author：
Hongwu Huang, Zhenzhen Liu, Xiaoru Qi, Nailong Gao, Jianguo Chang, Miaomiao Yang, Sha Na, Yanyan Liu, Rui Song, Lu Li, Guangliang Chen, Hui Zhou
IF：
4.3600
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
34343647
A novel vesicular stomatitis virus armed with IL-2 mimic for oncolytic therapy
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Author：
Manman Wu, Yiwei Wang, Chuanjian Wu, Huang Huang, Xinyuan Zhou, Jun Wang, Sidong Xiong, Chunsheng Dong
IF：
4.3000
Publish_to：
VIROLOGICA SINICA
PMID：
39299564
Molluscicidal activity and biochemical impacts of borrelidins against an aquatic invasive snail Pomacea canaliculata for crop protection
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Author：
Jingyan Wang, Zhihang Shi, Zihuan Wu, Han Wang, Huan Qi, Qiang Sheng, Shaoyong Zhang, Junhuan Song, Jidong Wang, Liqin Zhang, Chihang Cheng
IF：
4.2000
Publish_to：
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
PMID：
39277409
TBK1 participates in glutaminolysis by mediating the phosphorylation of RIPK3 to promote endotoxin tolerance
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Author：
Lehan Pan, Lian Yang, Zhujun Yi, Wenfeng Zhang, Jianping Gong
IF：
4.1740
Publish_to：
MOLECULAR IMMUNOLOGY
PMID：
35533409
Effects of rosemary extract and its residue on production, immune performance, and gut microbiota in geese
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Author：
Yuzhi Huang, Lanmeng Xu, Hang He, Lijuan Peng, Qinfeng Liao, Kun Wan, Simeng Qin, Lijing Cao, Jie Zhang
IF：
4.0000
Publish_to：
Frontiers in Microbiology
PMID：
39845044
Zearalenone Enhances TSST-1 Production by Intestinal Staphylococcus and Increases Uterine Immune Stress in Rats
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Author：
Ruiqi Zhang, Bingxin Huangfu, Tongxiao Xu, Victor Olusola Opatola, Qiushi Ban, Kunlun Huang, Xiaoyun He
IF：
3.9000
Publish_to：
FOOD AND CHEMICAL TOXICOLOGY
PMID：
39586525
Liver and Kidney Surgical Anatomy to Verify the Effect of miR-221 on Organ Damage in Septic Rats
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Author：
Bingtao Shi, Jialing Zhang, QiaoGe Chen
IF：
3.8220
Publish_to：
Journal of Healthcare Engineering
PMID：
35186224
Metformin ameliorates liver fibrosis induced by congestive hepatopathy via the mTOR/HIF-1α signaling pathway
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Author：
Jing Yang, Suxin Li, Shengyan Liu, Yuehui Zhang, Dongqi Shen, Peiju Wang, Xiaowei Dang
IF：
3.8000
Publish_to：
Annals of Hepatology
PMID：
37451514
Epigallocatechin-3-gallate Alleviates Vanadium-Induced Reduction of Antioxidant Capacity via Keap1-Nrf2-sMaf Pathway in the Liver, Kidney, and Ovary of Laying Hens
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Author：
Ma Yan, Shi Yizhen, Wu Qiujue, Ma Wenfeng
IF：
3.7380
Publish_to：
BIOLOGICAL TRACE ELEMENT RESEARCH
PMID：
33405082
Hif3α Plays Key Roles in the Progression of Alzheimer’s Disease Caused by Circadian Rhythm Disruption through Regulating the m6A/KDM3A/TGF-β1 Axis
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Author：
Xinrui Li, Zhengkun Han, Huiying Li
IF：
3.6000
Publish_to：
Biology-Basel
PMID：
38927292
Effects of myo-inositol on regulating glucose and lipid metabolism and alternative splicing events coexpressed with lncRNAs in the liver tissues of diabetic mice
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Author：
Jin'e Li, Qiulan Huang, Qin Nie, Yunfei Luo, Haixia Zeng, Yuying Zhang, Xiaoju He, Jianping Liu
IF：
3.4000
Publish_to：
Heliyon
PMID：
38933931
USP33 promotes nonalcoholic fatty acid disease-associated fibrosis in gerbils via the c-myc signaling
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Author：
Xianfu Ke, Huiying Hu, Qianyu Peng, Huazhong Ying, Xiaofeng Chu
IF：
3.3220
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
37267862
Delactylation diminished the growth inhibitory role of CA3 by restoring DUOX2 expression in hepatocellular carcinoma
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Author：
Jun Yan, Yunfei Zhou, Jianwen Xu, Yihong Dong, Xun Yang, Xinxin Yang, Aodi Wu, Shuyuan Chang, Yumeng Wang, Qingxin Zhang, Tomii Ayaka, Lei Yu, Liuyang Zhao, Hongxue Meng, Dabin Liu
IF：
3.3000
Publish_to：
EXPERIMENTAL CELL RESEARCH
PMID：
39710294
Pharmacological inhibition of Rac1 attenuates myocardial abnormalities in tail-suspended mice
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Author：
Li Huili, Cao Ting, Ding Weimin, Liang Liwen, Fan Guo-Chang, Qu Lina, Peng Tianqing
IF：
3.2160
Publish_to：
Journal of Cardiovascular Translational Research
PMID：
35088374
β-hydroxybutyrate administration improves liver injury and metabolic abnormality in postnatal growth retardation piglets
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Author：
Chengming Wang, Nan Wang, Yuankun Deng, Andong Zha, Junyao Li, Bie Tan, Ming Qi, Jing Wang, Yulong Yin
IF：
3.2000
Publish_to：
Frontiers in Veterinary Science
PMID：
38026634
Protective effects of SCFAs on organ injury and gut microbiota modulation in heat-stressed rats
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Author：
Yang Zhan, Tang Chengliang, Sun Xuewei, Wu Zihan, Zhu Xiaojing, Cui Qian, Zhang Ruonan, Zhang Xinrui, Su Yunxin, Mao Yinghua, Wang Chunhui, Zheng Feng, Zhu Jin
IF：
3.0000
Publish_to：
ANNALS OF MICROBIOLOGY
PMID：
Microbial creation of β-Nicotinamide mononucleotide and its regulation of lipid metabolism in the liver of high-fat diet mice
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Author：
Xutong Tian, Youyan Rong, Juyue Luo, Yaping Zhao, Tiantian Zhou, Yu Zeng, Kai Hong, Liang Jiang, Jianying Yang, Yumeng Li, Xin Wu
IF：
2.8000
Publish_to：
CELL BIOCHEMISTRY AND FUNCTION
PMID：
38953407
Selenium-Chitosan Treatment Affects Amino Acid Content and Volatile Components of Red Globe Grape during Storage
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Author：
Yaping Liu, Gang Ren, Xiaotong Zhao, Wei Wang, Jianbing Di, Yu Wang, Wenyan Lin
IF：
2.6090
Publish_to：
JOURNAL OF FOOD PROCESSING AND PRESERVATION
PMID：
Oroxylin A reverses SHP-2 oxidative inactivation in GPVI signaling to suppress platelet activation and thrombus formation
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Author：
Chen Yufei, Lin Yuan, Hong Jin, Wang Jiaorui, Li Biling, Liu Zixian, Ma Yongbo, Sun Xiaolan, Wang Shufang, Li Mingjie, Wu Meiling, Fu Deyu, Li Jian, Wang Mingzhu, Hu Liang
IF：
2.6000
Publish_to：
Thrombosis Journal
PMID：
40102927
Sesamin Attenuates Obesity-Associated Nonalcoholic Steatohepatitis in High-Fat and High-Fructose Diet-Fed Mice
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Author：
Yang Yang, Jing Yu, Jiayao Huo, Yaping Yan
IF：
2.4000
Publish_to：
JOURNAL OF MEDICINAL FOOD
PMID：
36637806
Krill Oil Ameliorates Liver Injury in Diabetic Mice by Activating Antioxidant Capacity and Inhibiting Ferroptosis
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Author：
Huali Meng, Jie Li, Yu Yang, Yan Zheng, Shue Wang, Xin Guo, Lei Du, Hao Wu
IF：
1.6000
Publish_to：
Journal of Oleo Science
PMID：
39085082
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