中文
英语
羟脯氨酸(HYP)含量检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:Hydroxyproline(HYP) Content Assay Kit
储存条件:Store at 2-8℃，6 months
产品简介
| 自备试剂 | 该试剂盒实验过程中需自备试剂，详情见网站说明书 |
| 中文名称 | 羟脯氨酸(HYP)含量检测试剂盒 |
| 英文名称 | Hydroxyproline(HYP) Content Assay Kit |
| 检测方法 | 可见分光光度法 Spectrophotometer |
| 储存条件 | Store at 2-8℃，6 months |
| 运输条件 | 冷藏运输 |
| 规格 | 50T/48S |
| 单位 | 盒 |
测定意义：HYP是机体胶原蛋白主要成分之一，胶原蛋白大多分布于皮肤、腱、软骨和血管等，因此HYP含量是反映胶原组织代谢及纤维化程度的一项重要指标。
测定原理：样品经水解产生游离的HYP，进一补被氯胺T氧化，氧化产物与对二甲氨基苯甲醛反应，产生红色化合物，在560nm处有特征吸收峰。通过测定样品水解液560nm吸光值，可计算HYP含量。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
Standard Curve for Hydroxyproline(HYP) Content Assay Kit
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Juan Li, Zhengcai Ma, Zhipeng Yang, Maochun Yang, Changsheng Li, Mengmeng Li, Xiaoduo Li, Xiantao Chen, Ma Hang, Wanqun Chen, Xiaoli Ye, Xuegang Li
IF：
5.4000
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
38103844
Polyphenol-Enriched Extract of Lacquer Sap Used as a Dentine Primer with Benefits of Improving Collagen Cross-Linking and Antibacterial Functions
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Author：
Ying Zhao, Xi He, Han Wang, Huimin Wang, Zuosen Shi, Song Zhu, Zhanchen Cui
IF：
5.3950
Publish_to：
ACS Biomaterials Science & Engineering
PMID：
35793160
Gynostemma Pentaphyllum ameliorates CCl4-induced liver injury via PDK1/Bcl-2 pathway with comprehensive analysis of network pharmacology and transcriptomics
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Author：
Linlan Hu, Xin Zhao, Xian He, Yafei Guo, Hanxiao Cheng, Shaoting Chen, Guangde Zhou, Jiabo Wang, Yawen Lu
IF：
5.3000
Publish_to：
Chinese Medicine
PMID：
38750545
Lingonberry Anthocyanins Inhibit Hepatic Stellate Cell Activation and Liver Fibrosis via TGFβ/Smad/ERK Signaling Pathway
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Author：
Guokun Zhang, Yunyao Jiang, Xin Liu, Yongyan Deng, Bin Wei, Liyan Shi
IF：
5.2790
Publish_to：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
34735147
Core fucosylation regulates alveolar epithelial cells senescence through activating of transforming growth factor-汕 pathway in pulmonary fibrosis
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Author：
Yu Jiang, Zhongzhen Wang, Jinying Hu, Wei Wang, Na Zhang, Lili Gao
IF：
5.2000
Publish_to：
Aging-US
PMID：
37724903
Quercetin promotes cutaneous wound healing in mice through Wnt/β-catenin signaling pathway
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Author：
Yuhui Mi, Lei Zhong, Saijian Lu, Po Hu, Yang Pan, Xuelin Ma, Binghui Yan, Zhenhuan Wei, Guangming Yang
IF：
5.1950
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
35122975
Discovery of LH10, a novel fexaramine-based FXR agonist for the treatment of liver disease
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Author：
Wanqiu Huang, Zhijun Cao, Wenxin Wang, Zhongcheng Yang, Shixuan Jiao, Ya Chen, Siliang Chen, Luyong Zhang, Zheng Li
IF：
5.1000
Publish_to：
BIOORGANIC CHEMISTRY
PMID：
38199141
Therapeutic siRNA targeting PLIN2 ameliorates steatosis, inflammation, and fibrosis in steatotic liver disease models
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Author：
Yao Wang, Jiaxin Zhou, Qi Yang, Xinmeng Li, Yifu Qiu, Yansong Zhang, Min Liu, Alan Jian Zhu
IF：
5.0000
Publish_to：
JOURNAL OF LIPID RESEARCH
PMID：
39187042
Interactions of two phosphate ester monomers with hydroxyapatite and collagen fibers and their contributions to dentine bond performance
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Author：
Fei Han, Xin Jin, Xiaojun Yuan, Zehua Bai, Qi Wang, Haifeng Xie
IF：
4.9910
Publish_to：
JOURNAL OF DENTISTRY
PMID：
35550398
MicroSPECT Imaging-Guided Treatment of Idiopathic Pulmonary Fibrosis in Mice with a Vimentin-Targeting 99mTc-Labeled N-Acetylglucosamine-Polyethyleneimine
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Author：
Deliang Zhang, Rongqiang Zhuang, Jindian Li, Yuting Lv, Xia Yang, Weimin Pan, Xianzhong Zhang
IF：
4.9390
Publish_to：
MOLECULAR PHARMACEUTICS
PMID：
34657437
Resveratrol Ameliorates Fibrosis in Rheumatoid Arthritis-Associated Interstitial Lung Disease via the Autophagy–Lysosome Pathway
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Author：
Lanxin Bao, Jing Ye, Nannan Liu, Yubao Shao, Wenhao Li, Xuefei Fan, Dahai Zhao, Hongzhi Wang, Xiaoyu Chen
IF：
4.9270
Publish_to：
MOLECULES
PMID：
36500562
Engineering Lipid Nanoparticles to Enhance Intracellular Delivery of Transforming Growth Factor-Beta siRNA (siTGF-β1) via Inhalation for Improving Pulmonary Fibrosis Post-Bleomycin Challenge
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Author：
Xu Deng, Yingjie Yang, Liming Gan, Xinliu Duan, Xiwei Wang, Jingyan Zhang, Aiping Wang, Anan Zhang, Zhizhao Yuan, Daquan Chen, Aiping Zheng
IF：
4.9000
Publish_to：
Pharmaceutics
PMID：
40006524
Rapid formation of 3D: Decellularized extracellular matrix spheroids for enhancing bone formation
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Author：
Yanmin Zhang, Yuting Feng, Qin Shao, Zhiwei Jiang, Guoli Yang
IF：
4.8540
Publish_to：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
PMID：
36355784
The engineered CRISPR-Mb2Cas12a variant enables sensitive and fast nucleic acid-based pathogens diagnostics in the field
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Author：
Zihui Tang, Bowen Lin, Wei Li, Xiaojuan Li, Fei Liu, Xinyan Zhu
IF：
4.8000
Publish_to：
CELLULAR SIGNALLING
PMID：
37290675
Purification and Identification of Novel Dipeptidyl Peptidase IV Inhibitory Peptides Derived from Bighead Carp (Hypophthalmichthys nobilis)
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Author：
Hanzhi Zheng, Leyan Zhao, Yushuo Xie, Yuqing Tan
IF：
4.7000
Publish_to：
Foods
PMID：
39272410
Extrafibrillar demineralization: Yes or no?
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Author：
Qingli Zhou, Yuzhu Li, Ting Xiao, Qi Zhong, Leping Wu, Zheng Zhou, Haiming Wong, Quan-Li Li
IF：
4.6000
Publish_to：
DENTAL MATERIALS
PMID：
38821839
Integrated analysis and validation of TRIM23/p53 signaling pathway in hepatic stellate cells ferroptosis and liver fibrosis
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Author：
Jie Chen, Rui Zhang, Feng Li, Shengli Lin, Jian Wang
IF：
4.5000
Publish_to：
DIGESTIVE AND LIVER DISEASE
PMID：
37495427
MSTN gene knockout suppresses the activation of lung fibroblasts through the inhibition of the Smad/AKT signaling pathway, thereby ameliorating pulmonary fibrosis
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Author：
Biao-Hu Quan, Xin-Yue Liu, Zhou-Yan Li, Sheng-Zhong Han, Qi-Chao Cui, Shuang-Yan Chang, Si-Tong Lv, Xi-Jun Yin, Shuang Liang
IF：
4.4000
Publish_to：
CELLULAR SIGNALLING
PMID：
39971222
Near-infrared Nrf2 activator IR-61 dye alleviates radiation-induced lung injury
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Author：
Jiancheng Zheng, Yang Wang, Ziwen Wang, Wanchao Chen, Min Luo, Can Zhang, Yawei Wang, Long Chen, Feng Wu, Wei Yang, Zeyu Yang, Yu Wang, Chunmeng Shi
IF：
4.3540
Publish_to：
FREE RADICAL RESEARCH
PMID：
36201846
Melatonin attenuates cholestatic liver injury via inhibition of the inflammatory response
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Author：
Tan Ya, Zhao Nan, Xie Qiaoling, Xu Ziqian, Chai Jin, Zhang Xiaoxun, Li Yan
IF：
4.3000
Publish_to：
MOLECULAR AND CELLULAR BIOCHEMISTRY
PMID：
36869985
Co-Mn Complex Oxide Nanoparticles as Potential Reactive Oxygen Species Scavenging Agents for Pulmonary Fibrosis Treatment
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Author：
Wuhao Yang, Hui Yuan, Hao Sun, Ting Hu, Yaping Xu, Yan Qiu, Yuhang Li
IF：
4.2000
Publish_to：
MOLECULES
PMID：
39519747
Macromolecular crowding enhances matrix proteins deposition in tissue engineered vascular grafts
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Author：
Qing Liu, Jiang Liu, Xu Heng Sun, Jian Yi Xu, Cong Xiao, Hong Jin Jiang, Yin Di Wu, Zhanyi Lin
IF：
4.1000
Publish_to：
Tissue Engineering Part A
PMID：
38318797
Elevated retinal fibrosis in experimental myopia is involved in the activation of the PI3K/AKT/ERK signaling pathway
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Author：
Bo Bao, Jinpeng Liu, Tuling Li, Zhaohui Yang, Guimin Wang, Jizhao Xin, Hongsheng Bi, Dadong Guo
IF：
3.9000
Publish_to：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
PMID：
37290701
Integrated strategy of RNA-sequencing and network pharmacology for exploring the protective mechanism of Shen-Shi-Jiang-Zhuo formula in rat with non-alcoholic fatty liver disease
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Author：
Zheng Xu, Fan-Wei Wu, Xuan Niu, Xiao-Peng Lu, Yan-Rong Li, Shu-Ting Zhang, Jun-Zhao Ou, Xue-Mei Wang
IF：
3.8890
Publish_to：
PHARMACEUTICAL BIOLOGY
PMID：
36124995
Comparative Analysis of Hyaline Cartilage Characteristics and Chondrocyte Potential for Articular Cartilage Repair
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Author：
Chiu Cheng, Zheng Kaiwen, Xue Mengxin, Du Dajiang
IF：
3.8000
Publish_to：
ANNALS OF BIOMEDICAL ENGINEERING
PMID：
38190025
Plunge-Freezing Cryopreservation of Tendons
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Author：
Haoyue Li, Xinmeng Liu, Liming Zhang, Lei Zhang
IF：
3.7000
Publish_to：
LANGMUIR
PMID：
38916446
Naringin regulates endoplasmic reticulum stress and mitophagy through the ATF3/PINK1 signaling axis to alleviate pulmonary fibrosis
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Author：
Wei Yi, Sun Lei, Liu Chao, Li Lujia
IF：
3.6000
Publish_to：
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
PMID：
36688958
Silica nanoparticle induces pulmonary fibroblast transdifferentiation via macrophage route: Potential mechanism revealed by proteomic analysis
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Author：
Ning Li, Luyao Wang, Fan Shi, Pan Yang, Kun Sun, Jing Zhang, Xiaojing Yang, Xiaoming Li, Fuhai Shen, Heliang Liu, Yulan Jin, Sanqiao Yao
IF：
3.5000
Publish_to：
TOXICOLOGY IN VITRO
PMID：
34271082
Enhanced osteogenicity of the demineralized bone-dermal matrix composite by the optimal partial demineralization for sustained release of bioactive molecules
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Author：
Tong Ma, Dangli Ren, Jingjing Wang, Feng Fu, Wendell Q. Sun, Hongtao Sun
IF：
3.4000
Publish_to：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
PMID：
38247243
m6A methyltransferase ZC3H13 improves pulmonary fibrosis in mice through regulating Bax expression
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Author：
Jing Guan, Lengyun Yin, Qi Huang, Jiamei Chen, Hui Liu, Jianmin Li
IF：
3.3000
Publish_to：
EXPERIMENTAL CELL RESEARCH
PMID：
39307407
Effect of Qiling Jiaogulan Powder on Pulmonary Fibrosis and Pulmonary Arteriole Remodeling in Low-Temperature-Exposed Broilers
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Author：
Juan Yu, Peng Li, Zhibian Duan, Xingyou Liu
IF：
3.2310
Publish_to：
Animals
PMID：
36611616
Myostatin deficiency decreases cardiac extracellular matrix in pigs
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Author：
Paek Hyo-Jin, Quan Biao-Hu, Choe Hak-Myong, Li Zhou-Yan, Yin Xi-Jun
IF：
3.1450
Publish_to：
TRANSGENIC RESEARCH
PMID：
35978205
USP33 promotes nonalcoholic fatty acid disease-associated fibrosis in gerbils via the c-myc signaling
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Author：
Xianfu Ke, Huiying Hu, Qianyu Peng, Huazhong Ying, Xiaofeng Chu
IF：
3.1000
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
37267862
miR-15a-5p regulates myocardial fibrosis in atrial fibrillation by targeting Smad7
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Author：
Dan He, Zhong-bao Ruan, Gui-xian Song, Ge-cai Chen, Fei Wang, Mei-xiang Wang, Mao-kun Yuan, Li Zhu
IF：
2.9840
Publish_to：
PeerJ
PMID：
35036160
5‑Aminosalicylic acid attenuates paraquat‑induced lung fibroblast activation and pulmonary fibrosis of rats
点击查看 >>
Author：
Hui Chen, Jinfeng Cui, Juan Wang, Yuan Wang, Fei Tong, Yingping Tian, Yu Gong, Yu Ma, Liang Liu, Xianghong Zhang
IF：
2.9520
Publish_to：
Molecular Medicine Reports
PMID：
34913070
Potential cardioprotective effect of paroxetine against ventricular remodeling in an animal model of myocardial infarction: a comparative study
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Author：
Alonazi Asma S., Almodawah Sara, Aldigi Rana, Bin Dayel Anfal, Alamin Maha, Almotairi Ahmad R., El-Tohamy Maha F., Alharbi Hana, Ali Rehab, Alshammari Tahani K., Alrasheed Nouf M.
IF：
2.8000
Publish_to：
BMC Pharmacology & Toxicology
PMID：
39696491
Cepharanthine attenuates pulmonary fibrosis via modulating macrophage M2 polarization
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Author：
Bao Jiaqi, Liu Chang, Song Huafeng, Mao Zheying, Qu Wenxin, Yu Fei, Shen Yifei, Jiang Jingjing, Chen Xiao, Wang Ruonan, Wang Qi, Chen Weizhen, Zheng Shufa, Chen Yu
IF：
2.6000
Publish_to：
BMC Pulmonary Medicine
PMID：
39261812
T1/T2 mapping as a non-invasive method for evaluating liver fibrosis based on correlation of biomarkers: a preclinical study
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Author：
Shuqin Xue, Yujie Zhu, Min Shao, Kun Zhu, Jing Rong, Tongtong Liu, Xiujuan Yin, Saisai Zhang, Likang Yin, Xiao Wang
IF：
2.5000
Publish_to：
BMC GASTROENTEROLOGY
PMID：
40016673
Effect of glabridin combined with bakuchiol on UVB-induced skin damage and its underlying mechanism: An experimental study
点击查看 >>
Author：
Guanjie Peng, Yangsi Li, Yiyan Zeng, Bowen Sun, Lanyue Zhang, Qingqing Liu
IF：
2.3000
Publish_to：
Journal of Cosmetic Dermatology
PMID：
38497297
Effects of Chemical Sterilization and Gamma Irradiation on the Biochemical and Biomechanical Properties of Human Tendon Allografts In Vitro Study
点击查看 >>
Author：
Hao-ran Zhang, Ming-you Xu, Lei Zhang, Hao Zhang, Li Yang, Jie Liu, Jing-yu Zhang, Yong-cheng Hu
IF：
2.2790
Publish_to：
Orthopaedic Surgery
PMID：
36054510
Angiosome-Guided Perfusion Decellularization of Fasciocutaneous Flaps
点击查看 >>
Author：
Liya Yang, Xueshan Bai, Yuanbo Liu, Shanshan Li, Zixiang Chen, Tinglu Han, Shenyang Jin, Tingjun Xie, Danying Wang, Shuai Yue, Miao Wang, Shan Zhu, Mengqing Zang
IF：
2.2000
Publish_to：
JOURNAL OF RECONSTRUCTIVE MICROSURGERY
PMID：
39191422
Effects of demineralization mode and particle size of allogeneic bone powder on its physical and chemical properties
点击查看 >>
Author：
Song Kun-xiu, Ji Shao-lin, Zhao Yong-jie, Zhang Hao-ran, Ma Rong-xing, Zhang Jing-yu, Hu Yong-cheng
IF：
1.7520
Publish_to：
CELL AND TISSUE BANKING
PMID：
35831637
Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy
点击查看 >>
Author：
Shi Sui, Yang Hou
IF：
1.5890
Publish_to：
SCANDINAVIAN CARDIOVASCULAR JOURNAL
PMID：
34296634
Repair of tendons treated with peracetic acid–ethanol and gamma irradiation by EDC combined with NHS: a morphological, biochemical and biomechanical study in vitro
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Author：
Ma Rong-xing, Li Rui-feng, Deng Xiao-qiang, Qiao Rui-qi, Li Ji-kai, Song Kun-xiu, Ji Shao-lin, Hu Yong-cheng
IF：
1.5000
Publish_to：
CELL AND TISSUE BANKING
PMID：
36797536
技术问答
（6）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
京公网安备 11011202000391号
可以用酶标仪做最后检测吗？需要加多少uL能检测出?2023-10-20 08:19:18
您好，不推荐缩减反应体系，如果用酶标仪检测有BC0255适配酶标仪体系微量法的试剂盒。
显色时间过长有什么影响?2023-10-17 15:02:25
您好，提取液中有易挥发成分，显色时间过长会导致体系浓度过高，最后读取的吸光度值不准确。
检测样品怎么处理?2023-08-08 08:44:14
您好，可以参考说明书中样本处理的操作步骤。
没有可见光分度计可不可以用酶标仪代替？可以用普通的96孔板检测吗?2023-07-12 08:46:03
您好，推荐BC0255微量法的试剂盒，该试剂盒适配酶标仪检测体系。
溶液中钙离子和磷酸根离子会对结果有影响吗?2023-03-11 21:33:19
理论上是不受影响的。