中文
英语
产品简介
| 中文名称 | 尼氏染色试剂盒(甲基紫法) |
| 英文名称 | Nissl Stain Kit(Methyl Violet Method) |
| 储存条件 | RT，避光保存，有效期6个月。 |
| 货期 | 2-3天 |
| 规格 | 2*50ml |
| 单位 | 盒 |
神经元细胞体包括一个具有皱褶核膜的大细胞核、稀疏的染色质和一个明显的核仁。在细胞体中细胞质是尼氏颗粒，即能够代表粗面内质网并在很多神经元中产生特异的斑点状嗜碱性表现的嗜碱性颗粒。尼氏颗粒可以用很多染色来显示如中性红、亚甲基蓝、甲苯胺蓝和甲基紫等。染色的变异、pH和分化的时间使一些染色既可以仅突出尼氏物质，也可以显示神经元的细胞核和神经胶质。
尼氏体(Nissl body)或称尼氏小体是分布于神经细胞胞质内的三角形或椭圆形小块状物质，能被碱性染料如硫堇、亚甲蓝、甲苯胺蓝和焦油紫等染料染成紫蓝色。各种神经细胞内都含有尼氏体，但其形状、数量、分布位置常常不同。尼氏体也存在于树突中，但不在于轴突和包体的轴丘。尼氏体会因为生理状态的变化而变化，尼氏体是神经元内合成蛋白质合成的重要部位，当神经元受到刺激后，包体内的尼氏体会明显减少。
尼氏染色试剂盒(甲基紫法)主要特点是操作简便、染色稳定、分化时间短，适用范围广，可以用于石蜡组织切片的尼氏物质、神经元等的染色，尼氏体的存在和消失是神经细胞是否受损的重要指标，当发生脑炎、脑缺血、轴突反应等情况时，尼氏体会发生溶解甚至消失。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
小鼠脑石蜡切片尼氏体着紫红色，背景不着色或着浅紫色。
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JOURNAL OF ETHNOPHARMACOLOGY
PMID：
38906338
Fecal microbiota transplantation ameliorates stress-induced depression-like behaviors associated with the inhibition of glial and NLRP3 inflammasome in rat brain
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Author：
Jingjing Rao, Yi Qiao, Ruining Xie, Li Lin, Jian Jiang, Chunmei Wang, Gongying Li
IF：
4.7910
Publish_to：
JOURNAL OF PSYCHIATRIC RESEARCH
PMID：
33677218
Protopine Exerts Neuroprotective Effects on Neonatal Hypoxic-Ischemic Brain Damage in Rats via Activation of the AMPK/PGC1α Pathway
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Author：
Liying Lu, Mengdan Pang, Tingting Chen, Yingying Hu, Likai Chen, Xiaoyue Tao, Shangqin Chen, Jianghu Zhu, Mingchu Fang, XiaoLing Guo, Zhenlang Lin
IF：
4.7000
Publish_to：
Drug Design Development and Therapy
PMID：
39525050
Extracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs.
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Author：
Lily Wan, Rou-Jie Huang, Chen Yang, Jia-Qi Ai, Qian Zhou, Jiao-E Gong, Jian Li, Yun Zhang, Zhao-Hui Luo, Ewen Tu, Aihua Pan, Bo Xiao, Xiao-Xin Yan
IF：
4.6770
Publish_to：
Frontiers in Neuroscience
PMID：
34916901
Tu-Xian Decoction ameliorates diabetic cognitive impairment by inhibiting DAPK-1
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Author：
Danyang WANG, Bin YAN, An WANG, Qing SUN, Junyi PANG, Yangming CUI, Guoqing TIAN
IF：
4.6000
Publish_to：
Chinese Journal of Natural Medicines
PMID：
38143108
Quercetin Protects Against Global Cerebral ischemia reperfusion Injury by Inhibiting Microglial Activation and Polarization
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Author：
Naigeng Wang, Fei Li, Jing Du, Jianhong Hao, Xin Wang, Yueru Hou, Zhenguo Luo
IF：
4.5000
Publish_to：
Journal of Inflammation Research
PMID：
38434580
Oxymatrine Alleviates Hyperglycemic Cerebral Ischemia/Reperfusion Injury via Protecting Microvessel
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Author：
Wang Wen-Jun, Ma Yan-Mei, He Mao-Tao, Zhang Deng-Hai, Wang Rui, Jing Li, Zhang Jian-Zhong
IF：
4.4140
Publish_to：
NEUROCHEMICAL RESEARCH
PMID：
35099719
Inhibition of CK2 Diminishes Fibrotic Scar Formation and Improves Outcomes After Ischemic Stroke via Reducing BRD4 Phosphorylation
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Author：
Li Xuemei, Yang Qinghuan, Jiang Peiran, Wen Jun, Chen Yue, Huang Jiagui, Tian Mingfen, Ren Jiangxia, Yang Qin
IF：
4.4000
Publish_to：
NEUROCHEMICAL RESEARCH
PMID：
38381246
Human Neural Stem Cell Secretome Inhibits Lipopolysaccharide-Induced Neuroinflammation Through Modulating Microglia Polarization by Activating Peroxisome Proliferator-Activated Receptor Gamma
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Author：
Jiqin Zhou, Wei Ni, Yating Ling, Xiaorui Lv, Dongdong Niu, Yu Zeng, Yun Qiu, Yu Si, Ziyu Wang, Jiabo Hu
IF：
4.3900
Publish_to：
STEM CELLS AND DEVELOPMENT
PMID：
35481777
Delivery of neurotrophin-3 by RVG-Lamp2b-modified mesenchymal stem cell-derived exosomes alleviates facial nerve injury
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Author：
Bi Wenting, Mu Xiaodan, Li Yongfeng, Sun Qingyan, Xiang Lei, Hu Min, Liu Huawei
IF：
4.3000
Publish_to：
Human Cell
PMID：
38858338
Deficiency of Autism-Related Gene Dock4 Leads to Impaired Spatial Memory and Hippocampal Function in Mice at Late Middle Age
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Author：
Guo Daji, Yang Xiaoman, Gao Ming, Chen Xiaoqing, Tang Yanping, Shen Lingling, Li Keshen, Shi Lei
IF：
4.2310
Publish_to：
CELLULAR AND MOLECULAR NEUROBIOLOGY
PMID：
35635601
Neuroimmune regulation of IFITM3 via γ-secretase in astrocytes during cerebral ischemia-reperfusion
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Author：
Xuqing Ni, Yuanyuan Pan, Xia Liu, Ye Zhu, Xinyu Yao, Yunchang Mo, Qinxue Dai, Junlu Wang
IF：
4.2000
Publish_to：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
PMID：
40020531
Zinc supplementation ameliorates sorafenib-induced cognitive impairment through ROS/JNK signaling pathway
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Author：
Zhou Can-Can, He Yu-Qiong, Qiu Yu-Shuang, Ni Chen-Xu, Shen Fu-Ming, Li Dong-Jie
IF：
4.0810
Publish_to：
BIOLOGICAL TRACE ELEMENT RESEARCH
PMID：
35129807
DNMT3b-mediated CpA methylation facilitates REST binding and gene silencing and exacerbates hippocampal demyelination in diabetic mice
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Author：
Tie-Feng Yao, Zhi-Yun Wang, Lu Sun, Sheng-Xue Yu, Hong Dan Yu, Zheng-Zhong Yang, Wan-Ze Li, Lin Niu, Die Sun, Ya-Hui Shi, Jun-Qi Li, Wen-Qiang Liu, Xue-Zheng Liu, Zhong-Fu Zuo
IF：
4.0000
Publish_to：
JOURNAL OF BIOLOGICAL CHEMISTRY
PMID：
39730060
The neuroprotective role of morroniside against spinal cord injury in female rats
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Author：
Fei-Xiang Duan, Yu-Jiao Shi, Jing Chen, Xue Song, Lin Shen, Qi Qi, Shu-Qin Ding, Qi-Yi Wang, Rui Wang, He-Zuo Lü, Jian-Guo Hu
IF：
3.9210
Publish_to：
NEUROCHEMISTRY INTERNATIONAL
PMID：
34147513
Sevoflurane Postconditioning Ameliorates Neuronal Migration Disorder Through Reelin/Dab1 and Improves Long-term Cognition in Neonatal Rats After Hypoxic-Ischemic Injury
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Author：
Zhang Yahan, Gao Qiushi, Wu Ziyi, Xue Hang, Zhao Ping
IF：
3.9110
Publish_to：
NEUROTOXICITY RESEARCH
PMID：
34224102
Up-regulating microRNA-214-3p relieves hypoxic–ischemic brain damage through inhibiting TXNIP expression
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Author：
Zhang Miaoyu, Zhou Haiyang, He Rongni, Yang Juan, Zou Yang, Deng Yiting, Xie Huifang, Yan Zhenxing
IF：
3.8420
Publish_to：
MOLECULAR AND CELLULAR BIOCHEMISTRY
PMID：
35980563
Wogonin alleviates NLRP3 inflammasome activation after cerebral ischemia-reperfusion injury by regulating AMPK/SIRT1
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Author：
Zhijuan Cheng, Jianglong Tu, Kai Wang, Fang Li, Yuan He, Wei Wu
IF：
3.8000
Publish_to：
BRAIN RESEARCH BULLETIN
PMID：
38253131
Antibacterial Effect of Chrysanthemum Buds' Crude Extract Against Salmonella Typhimurium and Potential Application in Cooked Chicken
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Author：
Min Xing, Jiyun Yao, Yiming Guo, Ru Xin, Yaping Yu, Encong Shi, Mengdi Hao, Peng Fei, Huaibin Kang, Junliang Chen
IF：
3.7880
Publish_to：
Foodborne Pathogens and Disease
PMID：
35363059
MicroRNA-27a-3p relieves inflammation and neurologic impairment after cerebral ischaemia reperfusion via inhibiting lipopolysaccharide induced TNF factor and the TLR4/NF-κB pathway
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Author：
Jing Luo, Junjie Li, Li Xiong, Linna Fan, Lijia Peng, Yuan Yang, Di Lu, Jianlin Shao
IF：
3.6980
Publish_to：
EUROPEAN JOURNAL OF NEUROSCIENCE
PMID：
35584745
Dynamic cerebral blood flow changes with FOXOs stimulation are involved in neuronal damage associated with high-altitude cerebral edema in mice
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Author：
Huaxiang Shi, Pengfei Li, Hu Zhou, Zhiyong Nie, Jingxin Zhang, Xin Sui, Jiabin Guo, Yongan Wang, Liyun Wang
IF：
3.6100
Publish_to：
BRAIN RESEARCH
PMID：
35724762
Peptide OM-LV20 promotes structural and functional recovery of spinal cord injury in rats
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Author：
Jian Zhao, Ailang Pang, Saige Yin, Meifeng Yang, Xuemei Zhang, Rong Zhang, Jingfei Liu, Yuanqi Gu, Shanshan Li, Yan Hu, Yue Zhang, Yingchun Ba, Buliang Meng, Xinwang Yang
IF：
3.3220
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
35158211
GABAB Receptor Activation Attenuates Neuronal Pyroptosis in Post-cardiac Arrest Brain Injury
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Author：
Ye Sun, Jinying Li, Haikuo Wu, Ziwei Zhao, Ting Cong, Liya Li, Xiaonan Zhang, Shengming Yin, Zhaoyang Xiao
IF：
3.3000
Publish_to：
NEUROSCIENCE
PMID：
37352966
Bone mesenchymal stem cell-derived extracellular vesicles deliver microRNA-23b to alleviate spinal cord injury by targeting toll-like receptor TLR4 and inhibiting NF-κB pathway activation
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Author：
Hongfei Nie, Zhensong Jiang
IF：
3.2690
Publish_to：
Bioengineered
PMID：
34663169
Berberine Ameliorates Lipopolysaccharide-Induced Cognitive Impairment Through SIRT1/NRF2/NF-κB Signaling Pathway in C57BL/6J Mice
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Author：
Nan Chen, Xin-Chen Wang, Ling-Ling Fan, Yu-Huang Zhu, Qi Wang, Yun-Bo Chen
IF：
3.1920
Publish_to：
REJUVENATION RESEARCH
PMID：
36029207
Protective Role of TRPV2 in Synaptic Plasticity Through the ERK1/2-CREB-BDNF Pathway in Chronic Unpredictable Mild Stress Rats
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Author：
Yitong Zhou, Ting Cong, Jun Chen, Zhenchen Chu, Ye Sun, Danmei Zhao, Xue Chen, Liya Li, Yingxin Liu, Jiani Cheng, Qiwei Li, Shengming Yin, Zhaoyang Xiao
IF：
3.1000
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
38776831
Antidepressant-like effects of Rehmannioside A on rats induced by chronic unpredictable mild stress through inhibition of endoplasmic reticulum stress and apoptosis of hippocampus
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Author：
Mei Yuan, Bozhi Yuan
IF：
3.0970
Publish_to：
JOURNAL OF CHEMICAL NEUROANATOMY
PMID：
36067970
Ozone rectal insufflation mitigates chronic rapid eye movement sleep deprivation-induced cognitive impairment through inflammation alleviation and gut microbiota regulation in mice
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Author：
Cheng Jie, Williams John P., Zhou Li, Wang Peng-Cheng, Sun Li-Na, Li Rui-Hua, An Jian-Xiong
IF：
3.0000
Publish_to：
Medical Gas Research
PMID：
39073330
Forsythoside B alleviates cerebral ischemia-reperfusion injury via inhibiting NLRP3 inflammasome mediated by SIRT1 activation
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Author：
Qiaoyu Li, Chongyang Zhang, Xiao Sun, Mengchen Wang, Zhixiu Zhang, Rongchang Chen, Xiaobo Sun
IF：
2.9000
Publish_to：
PLoS One
PMID：
38885233
Glyphosate Induces Anxiety-Like Behaviors in Mice via Activating NLRP3-Mediated Hippocampal Microglia Pyroptosis
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Author：
Shuge Shu, Xinting Chen, Jiawen Ren, Xiangyu Yu, Hao Zhang, Yongquan Yu
IF：
2.7000
Publish_to：
JOURNAL OF APPLIED TOXICOLOGY
PMID：
40254291
Protective effect of glutamic-oxaloacetic transaminase on hippocampal neurons in Alzheimer's disease using model mice
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Author：
Haiming Li, Dainan Zhang, Xiaoze Wang, Shensheng Wang, Meng Xiao
IF：
2.5000
Publish_to：
NEUROSCIENCE LETTERS
PMID：
36931592
Oscillating field stimulation promotes recovery from spinal cord injury in rats by regulating the differentiation of endogenous neural stem cells
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Author：
Chao Fang, Jian Sun, Laifu Wei, Fei Gao, Jun Qian
IF：
2.4470
Publish_to：
Experimental and Therapeutic Medicine
PMID：
34345261
Electroacupuncture Relieves Hippocampal Injury by Heme Oxygenase-1 to Improve Mitochondrial Function
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Author：
Rui Mu, Na Li, Jian-bo Yu, Li-rong Gong, Shu-an Dong, Jia Shi, Yuan Zhang, Zi-lei Xie
IF：
2.4170
Publish_to：
JOURNAL OF SURGICAL RESEARCH
PMID：
35016152
LRP1-mediated p-tau propagation contributes to cognitive impairment after chronic neuropathic pain in rats
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Author：
Youzhi Ning, Yue Zhang, Tao Jiang, Jianguo Feng, Jian Zhan, Cehua Ou, Lu Wang
IF：
2.4000
Publish_to：
NEUROSCIENCE RESEARCH
PMID：
39674403
Lipid nanoparticle-encapsulated lncRNA DLX6-AS1 knockdown ameliorates cerebral ischemic injury via the Nrf2/HO-1/NLRP3 axis
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Author：
Chang Song, Yan Li, Huiying Han, Yueyue Zhang, Ning Wang
IF：
1.7000
Publish_to：
NEUROLOGICAL RESEARCH
PMID：
38735062
Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis
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Author：
Jiang Xi, Yu Xuefeng, Chen Jin, Jing Changfeng, Xu Lexing, Chen Ziwei, Liu Fuhe, Chen Lei
IF：
1.3880
Publish_to：
Acta Cirurgica Brasileira
PMID：
34495140
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