低密度脂蛋白胆固醇(LDL-C)含量检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Low-Density Lipoprotein Cholesterol(LDL-C)Content Assay kit
储存条件:Store at 2-8℃,6 months


产品简介
自备试剂 | 该试剂盒实验过程中需自备试剂,详情见网站说明书 |
英文名称 | Low-Density Lipoprotein Cholesterol(LDL-C)Content Assay kit |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at 2-8℃,6 months |
规格 | 100T/96S |
单位 | 盒 |
低密度脂蛋白是血清脂蛋白中胆固醇含量最高的一种脂蛋白,其颗粒较小,主要作用是将肝脏组织的胆固醇经过血液转运至其他组织,促进组织细胞中胆固醇的积累。众多流行病学研究表明,血清低密度脂蛋白胆固醇(Low-Density Lipoprotein Cholesterol,LDL-C)水平与动脉粥样硬化(AS)、冠心病(GHD)呈正相关,对于临床诊断动脉粥硬化、冠心病、高血压等疾病有重要参考价值。
使用选择性表面活性剂,特异性解离出CM、VLDL、HDL中的胆固醇,而不作用于LDL,解离出的胆固醇与胆固醇酯酶(CHER)和胆固醇氧化酶(CHOD)反应产生H2O2,在缺乏显色剂的情况下被消耗掉而不显色;未被分解的LDL在另一特异性表面活性剂的作用下解离,利用酯酶催化胆固醇酯水解生成游离胆固醇(FC)和游离脂肪酸(FFA),从而把胆固醇酯转化为FC;进一步利用胆固醇氧化酶催化FC氧化,生成4-胆甾烯酮和H2O2;最后利用过氧化物酶催化H2O2氧化4-氨基安替比林和苯胺类似物,生成紫色化合物,其在546nm有特征吸收峰。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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