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总胆固醇(TC)含量检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:Total Cholesterol(TC) Content Assay Kit
储存条件:Store at 2-8℃，6 months
产品简介
| 自备试剂 | 该试剂盒实验过程中需自备试剂，详情见网站说明书 |
| 中文名称 | 总胆固醇(TC)含量检测试剂盒 |
| 英文名称 | Total Cholesterol(TC) Content Assay Kit |
| 检测方法 | 可见分光光度法 Spectrophotometer |
| 储存条件 | Store at 2-8℃，6 months |
| 规格 | 50T/48S |
| 单位 | 盒 |
测定意义：TC 包括游离胆固醇和胆固醇酯。TC 是指组织中所有脂蛋白所含胆固醇之总和。
测定原理：利用酯酶催化胆固醇酯水解生成游离胆固醇（FC）和游离脂肪酸（FFA），从而把胆固醇酯转化为FC；进一步利用胆固醇氧化酶催化FC 氧化，生成△4-胆甾烯酮和H2O2；最后利用过氧化物酶催化H2O2 氧化4-氨基安替比林和酚，生成红色醌类化合物；在500nm 有特征吸收峰，其颜色深浅与TC 含量成正比。
自备仪器和用品：可见分光光度计、可调式移液枪、1mL 玻璃比色皿、异丙醇和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
Standard Curve for Total Cholesterol(TC) Content Assay Kit
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Cannabidiol (CBD) Inhibits Foam Cell Formation via Regulating Cholesterol Homeostasis and Lipid Metabolism
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Author：
Mengxue He, Jiachen Shi, Yong-Jiang Xu, Yuanfa Liu
IF：
4.5000
Publish_to：
MOLECULAR NUTRITION & FOOD RESEARCH
PMID：
38932553
Ultrabright silicon nanoparticle fluorescence probe for sensitive detection of cholesterol in human serum
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Author：
Ye Xiwen, Jiang Yanxiao, Mu Xiaowei, Sun Ying, Ma Pinyi, Ren Ping, Song Daqian
IF：
4.4780
Publish_to：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
PMID：
35347354
Synthesis and biological evaluation of the novel chrysin prodrug for nonalcoholic fatty liver disease treatment
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Author：
Zhang Ruiming, Gao Chuanyue, Hu Mingxing, Wang Xingxing, Li Shuoyuan, An Zhenmei, Yang Xifei, Xie Yongmei
IF：
4.4000
Publish_to：
Frontiers in Pharmacology
PMID：
38708081
High uric acid induces liver fat accumulation via ROS/JNK/AP-1 signaling
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Author：
De Xie, Hairong Zhao, Jiaming Lu, Furong He, Weidong Liu, Wei Yu, Qiang Wang, Ichiro Hisatome, Tetsuya Yamamoto, Hidenori Koyama, Jidong Cheng
IF：
4.3100
Publish_to：
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
PMID：
33900847
Searching for Hub Genes of Quince–Basil Co-Administration Against Atherosclerosis Using Bioinformatics Analysis and Experimental Validation
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Author：
Sendaer Hailati, Meng-Yuan Han, Dilihuma Dilimulati, Nuerbiye Nueraihemaiti, Alhar Baishan, Alifeiye Aikebaier, Wen-Ting Zhou
IF：
4.3000
Publish_to：
Pharmaceuticals
PMID：
39598345
Adipose-specific BMP and activin membrane-bound inhibitor (BAMBI) deletion promotes adipogenesis by accelerating ROS production
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Author：
Xiaochang Chen, Chen Zhao, Yanting Xu, Kuilong Huang, Yulong Wang, Xiaoyu Wang, Xiaoge Zhou, Weijun Pang, Gongshe Yang, Taiyong Yu
IF：
4.2380
Publish_to：
JOURNAL OF BIOLOGICAL CHEMISTRY
PMID：
33158991
Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling
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Author：
Rui Wang, Dan-Feng Li, Yi-Fang Hu, Qiang Liao, Tian-Tian Jiang, Opeyemi Joshua Olatunji, Kui Yang, Jian Zuo
IF：
4.1620
Publish_to：
Drug Design Development and Therapy
PMID：
34295151
Toxicity of thioacetamide and protective effects of quercetin in zebrafish (Danio rerio) larvae
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Author：
Kexin Wang, Yunyun Deng, June Zhang, Bo Cheng, Yong Huang, Yunlong Meng, Keyuan Zhong, Guanghua Xiong, Jing Guo, Yi Liu, Huiqiang Lu
IF：
4.1190
Publish_to：
ENVIRONMENTAL TOXICOLOGY
PMID：
34227734
CTRP9 prevents atherosclerosis progression through changing autophagic status of macrophages by activating USP22 mediated-de-ubiquitination on Sirt1 in vitro
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Author：
Min Zeng, Yali Yang, Ziyan Wang, Xiuyang Zhao, Dianshu Zhu, Mengdi Wang, Yue Chen, Xin Wei
IF：
4.1000
Publish_to：
MOLECULAR AND CELLULAR ENDOCRINOLOGY
PMID：
38280475
Synthetic ditempolphosphatidylcholine liposome-like nanoparticles for anti-oxidative therapy of atherosclerosis
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Author：
Chunxiao Wang, Ruifu Zhao, Zhen Wang, Tingting Xu, Peng Huang
IF：
4.0360
Publish_to：
RSC Advances
PMID：
37266511
Effects of rosemary extract and its residue on production, immune performance, and gut microbiota in geese
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Author：
Yuzhi Huang, Lanmeng Xu, Hang He, Lijuan Peng, Qinfeng Liao, Kun Wan, Simeng Qin, Lijing Cao, Jie Zhang
IF：
4.0000
Publish_to：
Frontiers in Microbiology
PMID：
39845044
Effect of administration routes on the efficacy of human umbilical cord mesenchymal stem cells in type 2 diabetic rats
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Author：
Qiqiang Tao, Youzhi Wu, Huiwen Pang, Pinglei Lv, Wenrui Li, Xuqiang Nie, Felicity Y. Han
IF：
3.9000
Publish_to：
Frontiers in Endocrinology
PMID：
40190404
Overexpression CPT1A reduces lipid accumulation via PPARα/CD36 axis to suppress the cell proliferation in ccRCC: CPT1A inhibits ccRCC cell proliferation via PPARα/CD36 axis
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Author：
Hui Yang, Hongbo Zhao, Zhongkun Ren, Xiaojia Yi, Qiao Zhang, Zhe Yang, Yingmin Kuang, Yuechun Zhu
IF：
3.8480
Publish_to：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
PMID：
35130611
Hydroxysafflor yellow A inhibits endothelial cell ferroptosis in diabetic atherosclerosis mice by regulating miR-429/SLC7A11
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Author：
Jianjie Rong, Chuanyong Li, Qiang Zhang, Guangfeng Zheng, Weijian Fan, Zhichang Pan, Shuming Shi
IF：
3.8000
Publish_to：
PHARMACEUTICAL BIOLOGY
PMID：
37410531
The Adaptive Characteristics of Cholesterol and Bile Acid Metabolism in Nile Tilapia Fed a High-Fat Diet
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Author：
Rui-Xin Li, Yi-Fan Qian, Wen-Hao Zhou, Jun-Xian Wang, Yan-Yu Zhang, Yuan Luo, Fang Qiao, Li-Qiao Chen, Mei-Ling Zhang, Zhen-Yu Du
IF：
3.7810
Publish_to：
AQUACULTURE NUTRITION
PMID：
36860444
Early programming effects of a hyperglucidic diet on carbohydrate and lipid metabolism in gibel carp (Carassius gibelio)
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Author：
Hancheng Geng, Wenjie Xu, Liyun Wu, Bo Dong, Dong Han, Haokun Liu, Xiaoming Zhu, Yunxia Yang, Shouqi Xie, Junyan Jin
IF：
3.7000
Publish_to：
Aquaculture Reports
PMID：
Molecular mechanism of plant elicitor daphnetin-carboxymethyl chitosan nanoparticles against Ralstonia solanacearum by activating plant system resistance
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Author：
Zuo Jiangwei, Guo Sien, Qin Xiao
IF：
3.6000
Publish_to：
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
PMID：
37289282
MicroRNA-202-5p-dependent inhibition of Bcl-2 contributes to macrophage apoptosis and atherosclerotic plaque formation
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Author：
Fei Xu, Fang Yao, Yayuan Ning
IF：
3.5000
Publish_to：
GENE
PMID：
36931409
Limosilactobacillus fermentum TY-S11 ameliorates hypercholesterolemia via promoting cholesterol excretion and regulating gut microbiota in high-cholesterol diet-fed apolipoprotein E-deficient mice
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Author：
Yadan Deng, Jing Wang, Ran Wang, Yuying Wang, Xi Shu, Pengjie Wang, Chong Chen, Feng Zhang
IF：
3.4000
Publish_to：
Heliyon
PMID：
38882320
Huoxue Jiangtang Decoction Alleviates Type 2 Diabetes Mellitus by Regulating the Oral Microbiota and Food Preferences
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Author：
Qian Huang, Lu Meng, Huilin Li, Ni Xiong, Lin Zeng, Gaoxiang Wang, Pengxiang Zhang, Hengxia Zhao, Deliang Liu
IF：
3.2490
Publish_to：
Diabetes Metabolic Syndrome and Obesity-Targets and Therapy
PMID：
36474726
Effects of cholesterol-lowering probiotic fermentation on the active components and in vitro hypolipidemic activity of sea buckthorn juice
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Author：
Ying Fan, Yueling Shang, Fengwei Li, Jinbin Liu, Dujun Wang, Ya Zhang, Xiaohong Yu, Wei Wang
IF：
3.2000
Publish_to：
JOURNAL OF FOOD SCIENCE
PMID：
39223756
NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells
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Author：
Yu Hao, Li Xiaotong, Zhao Jie, Wang Wei, Wei Quanwei, Mao Dagan
IF：
3.1000
Publish_to：
BIOLOGY OF REPRODUCTION
PMID：
38936833
Exogenous Spermidine Alleviates Diabetic Myocardial Fibrosis Via Suppressing Inflammation and Pyroptosis in db/db Mice
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Author：
Can Wei, Jiyu Xu, Yong Liu, Javeria Qadir, Shumin Zhang, Hui Yuan
IF：
3.0000
Publish_to：
Balkan Medical Journal
PMID：
37350700
Protective Effect of Akkermansia muciniphila on the Preeclampsia-Like Mouse Model
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Author：
Liu Wei, Zhang Tingting, Wang Juanni, Zhao Gang, Hou Yuemin
IF：
2.9000
Publish_to：
Reproductive Sciences
PMID：
36920671
Microbial creation of β-Nicotinamide mononucleotide and its regulation of lipid metabolism in the liver of high-fat diet mice
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Author：
Xutong Tian, Youyan Rong, Juyue Luo, Yaping Zhao, Tiantian Zhou, Yu Zeng, Kai Hong, Liang Jiang, Jianying Yang, Yumeng Li, Xin Wu
IF：
2.8000
Publish_to：
CELL BIOCHEMISTRY AND FUNCTION
PMID：
38953407
Alternate-day fasting alleviates high fat diet induced non-alcoholic fatty liver disease through controlling PPARα/Fgf21 signaling
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Author：
Liu Xinlei, Zhang Yan, Ma Chunya, Lin Juntang, Du Jiang
IF：
2.7420
Publish_to：
MOLECULAR BIOLOGY REPORTS
PMID：
35107741
Functions of Monocytes and Macrophages and the Associated Effective Molecules and Mechanisms at the Early Stage of Atherosclerosis
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Author：
Liyuan Jin, Zihui Deng, Yongyi Bai, Ping Ye
IF：
2.6720
Publish_to：
Acta Cardiologica Sinica
PMID：
34584385
Laminaria japonica Polysaccharide Regulates Fatty Hepatosis Through Bile Acids and Gut Microbiota in Diabetes Rat
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Author：
Zhang Bo, Wang Jiacai, Chen Xiaoxue, Xue Tao, Xin Jie, Liu Yanjuan, Wang Xiao, Li Xinpeng
IF：
2.6000
Publish_to：
MARINE BIOTECHNOLOGY
PMID：
39207652
Sodium butyrate ameliorates non-alcoholic fatty liver disease by upregulating miR-150 to suppress CXCR4 expression
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Author：
Ning Zhang, Yunfei Qu, Bo Qin
IF：
2.5570
Publish_to：
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
PMID：
33721354
Roflumilast ameliorates GAN diet-induced non-alcoholic fatty liver disease by reducing hepatic steatosis and fibrosis in ob/ob mice
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Author：
Bin Wang, Xiaochan Zhu, Siting Yu, Huan Xue, Lijiao Deng, Yushan Zhang, Yi Zhang, Yunfeng Liu
IF：
2.5000
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
38797152
Inhibition of poly (ADP-ribose) polymerase 1 in neuron cells might enhance the therapeutic effect of leonurine under cholesterol stimulation
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Author：
Bo Hou, Zhigang Huan, Chao Zhang, Zhiyong Chen, Lingmei Ha, Shengxuan Huang
IF：
2.4940
Publish_to：
FOLIA NEUROPATHOLOGICA
PMID：
35359148
Emodin inhibits NF-κB signaling pathway to protect obese asthmatic rats from pathological damage via Visfatin
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Author：
Jing Miao, Xiaoming He, Jiang Hu, Wanru Cai
IF：
2.4660
Publish_to：
TISSUE & CELL
PMID：
34952398
Dexamethasone and azithromycin enhance goat sperm preservation quality by regulating lipid metabolism
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Author：
Yongjie Xu, Shixin Sun, Mingyue Wang, Wenzheng Shen, Lei Wang, Chunhuan Ren, Yinghui Ling, Zijun Zhang, Hongguo Cao
IF：
2.4000
Publish_to：
THERIOGENOLOGY
PMID：
39476552
PKMYT1 knockdown inhibits cholesterol biosynthesis and promotes the drug sensitivity of triple-negative breast cancer cells to atorvastatin
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Author：
Wei Gao, Xin Guo, Linlin Sun, Jinwei Gai, Yinan Cao, Shuqun Zhang
IF：
2.3000
Publish_to：
PeerJ
PMID：
39011373
Cholesterol crystals activate NLRP3 inflammasomes and promote gallstone formation by increasing mucin secretion
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Author：
You-Ming Lei, Ran Yan, Yue-Dong Gao, Hong-Ju Yang, Hong-Yan Bi, Yong-Qing Duan
IF：
1.8340
Publish_to：
BIOTECHNIC & HISTOCHEMISTRY
PMID：
35227133
Thrombospondin-2 induces M2 macrophage polarization through fatty acid metabolism to drive lung adenocarcinoma proliferation
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Author：
Weng Meiling, Zhu Xiaoping
IF：
1.8000
Publish_to：
ANTI-CANCER DRUGS
PMID：
40053399
Cichorium glandulosum Ameliorates HFD-Induced Obesity in Mice by Modulating Gut Microbiota and Bile Acids
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Author：
Yewei Zhong, Hurxida Emam, Wenhui Hou, Junlin Yan, Adalaiti Abudurexiti, Rui Zhang, Shuwen Qi, Yi Lei,Xiaoli Ma
IF：
1.7000
Publish_to：
JOURNAL OF MEDICINAL FOOD
PMID：
38742981
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