NADP-苹果酸脱氢酶(NADP-MDH)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:NADP-Malate Dehydrogenase(NAD-MDH) Activity Assay Kit
储存条件:Store at -20℃,6 months


产品简介
中文名称 | NADP-苹果酸脱氢酶(NADP-MDH)活性检测试剂盒 |
英文名称 | NADP-Malate Dehydrogenase(NAD-MDH) Activity Assay Kit |
别名 | NADP-苹果酸脱氢酶试剂盒 NADP-MDH Kit NADP-苹果酸脱氢酶(NADP-MDH)试剂盒 NADP-苹果酸脱氢酶(NADP-MDH)测试盒 |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at -20℃,6 months |
推荐 | 若使用96孔板测定,需使用96孔UV板,推荐货号YA0602 |
规格 | 100T/96S |
单位 | 盒 |
测定原理:
NADP-MDH催化NADPH还原草酰乙酸生成苹果酸,导致340nm处光吸收下降。
需要的仪器,耗材:
紫外分光光度计/酶标仪、台式离心机、水浴锅、可调式移液器、微量石英比色皿/96孔板和蒸馏水。
测定意义:
MDH (EC 1.1.1.37)广泛存在于动物、植物、微生物和培养细胞中,线粒体中MDH是TCA循环的关键酶之一,催化苹果酸形成草酰乙酸;相反,胞浆中MDH催化草酰乙酸形成苹果酸。草酰乙酸是重要的中间产物, 连接多条重要的代谢途径。因此,MDH在细胞多种生理活动中扮演着重要的角色,包括线粒体的能量代谢、 苹果酸-天冬氨酸穿梭系统、活性氧代谢和抗病性等。根据不同的辅酶特异性,MDH分为NAD-依赖的MDH和NADP-依赖的MDH, NADP-MDH主要存在于真核细胞中。测定原理:NADP-MDH催化NADPH还原草酰乙酸生成苹果酸,导致340nm处光吸收下降。需要的仪器,耗材:紫外分光光度计/酶标仪、台式离心机、水浴锅、可调式移液器、微量石英比色皿/96孔板和蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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