海藻糖含量检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Trehalose Content Assay Kit
储存条件:Store at 2-8℃,6 months
产品简介
自备试剂 | 该试剂盒实验过程中需自备试剂,详情见网站说明书 |
中文名称 | 海藻糖含量检测试剂盒 |
英文名称 | Trehalose Content Assay Kit |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at 2-8℃,6 months |
规格 | 100T/96S |
单位 | 盒 |
测定方法采用蒽酮比色法。具有简便快捷﹑适用于微量样品的测定等优点。但是蒽酮比色法也存在一定缺陷,如果样本中含有可溶性糖,则会影响测定。本试剂盒建议用于除海藻糖外不含其他可溶性糖样本的测定。
测定意义:海藻糖广泛存在于动物、植物、微生物和培养细胞中。由于海藻糖具有独特的不同于其他碳水化合物的生物学特性,能在干旱、高温、脱水、冷冻、高渗透压及毒性物质等恶劣环境下保护生物体细胞蛋白质、脂肪、糖类、核酸等组分不受损害。
测定原理:蒽酮比色法。具有灵敏度高﹑简便快捷﹑适用于微量样品的测定等优点。
需要的仪器,耗材:可见分光光度计/酶标仪、水浴锅、可调式移液器、微量石英比色皿/96孔板﹑研钵、浓硫酸(不允许快递)和蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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