总糖含量检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:Total Carbohydrate Content Assay Kit
储存条件:Store at 2-8℃,6 months
产品简介
中文名称 | 总糖含量检测试剂盒 |
英文名称 | Total Carbohydrate Content Assay Kit |
检测方法 | 可见分光光度法 Spectrophotometer |
储存条件 | Store at 2-8℃,6 months |
规格 | 50T/48S |
单位 | 盒 |
测定意义:
糖类物质是构成植物体的重要组成成分之一,也是新陈代谢的主要原料和贮存物质。总糖也可称为碳水化合物,包括可溶性的单糖,二糖以及不溶性的淀粉,纤维素,几丁质等。
测定原理:
总糖酸水解为还原糖,在NaOH 和丙三醇存在下,DNS 试剂与还原糖共热后被还原成氨基化合物,在过量的NaOH 碱性溶液中呈桔红色,在540nm 处有最大吸收峰,以此测定样品中的总糖含量。
需自备的仪器和用品:
可见分光光度计、沸水浴、可调式移液器、1mL 石英比色皿、研钵、蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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