柠檬酸合酶(CS)活性检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:Citrate Synthase(CS) Activity Assay Kit
储存条件:Store at -20℃,6 months


产品简介
中文名称 | 柠檬酸合酶(CS)活性检测试剂盒 |
英文名称 | Citrate Synthase(CS) Activity Assay Kit |
检测方法 | 可见分光光度法 Spectrophotometer |
储存条件 | Store at -20℃,6 months |
规格 | 10T/5S 25T/12S 50T/24S |
单位 | 盒 |
测定意义:CS(EC 2.3.3.1)广泛存在于动物、植物、微生物和培养细胞的线粒体基质中,是三羧酸循环第一个限速酶,是三羧酸循环主要调控位点之一。
测定原理:CS 催化乙酰CoA 和草酰乙酸产生柠檬酰辅酶A,进一步水解产生柠檬酸;该反应促使无色的DTNB 转变成黄色的TNB,在 412nm 处有特征吸光值。
需自备的仪器和用品:可见分光光度计、台式离心机、水浴锅、可调式移液器、1mL 玻璃比色皿、研钵、冰、无水乙醇和蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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