中文
英语
台盼蓝染色液(0.4%)
英文名称:Trypan Blue Stain Solution，0.4%
CAS:No.72-57-1
分子式:C34H24N6O14S4Na4
分子量:960.81
储存条件:2-8℃，有效期1年。
产品简介
| 中文名称 | 台盼蓝染色液(0.4%) |
| 英文名称 | Trypan Blue Stain Solution，0.4% |
| 别名 | 台盼蓝 |
| CAS | No.72-57-1 |
| 分子式 | C34H24N6O14S4Na4 |
| 分子量 | 960.81 |
| 储存条件 | 2-8℃，有效期1年。 |
| 规格 | 50ml 100ml |
| 单位 | 瓶 |
台盼蓝（Trypan Blue)或称台盼兰、锥虫蓝，是细胞活性染料，常用于检测细胞膜的完整性与细胞的存活率，是组织和细胞培养中最常用的死细胞鉴定染色方法之一。正常的活细胞细胞膜结构完整，能够排斥台盼蓝，细胞不会被染成蓝色；而丧失活性或细胞膜不完整的细胞可被台盼蓝染成蓝色。通常认为细胞膜完整性丧失，即可认为细胞已经死亡。
台盼蓝可被巨噬细胞吞噬，故可用于巨噬细胞的活体染色剂。凋亡小体也有台盼蓝拒染现象。台盼蓝染色后，通过显微镜下直接计数或显微镜下拍照后计数，就可以对细胞存活率进行比较精确的定量。染色时间只需3-5分钟，操作简单。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
培养3T3细胞活细胞呈无色，死细胞着蓝色
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Sodium Propionate Relieves LPS-Induced Inflammation by Suppressing the NF- B and MAPK Signaling Pathways in Rumen Epithelial Cells of Holstein Cows
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Author：
Chenxu Zhao, Fanxuan Yi, Bo Wei, Panpan Tan, Yan Huang, Fangyuan Zeng, Yazhou Wang, Chuang Xu, Jianguo Wang
IF：
4.2000
Publish_to：
Toxins
PMID：
37505707
Pasteurella multocida Serotype D Infection Induces Activation of the IL-17 Signaling Pathway in Goat Lymphocytes
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Author：
Yujing Fu, Yong Meng, Hejie Qian, Taoyu Chen, Xiangying Chen, Qiaoling Chen, Hongyan Gao, Churiga Man, Li Du, Si Chen, Fengyang Wang
IF：
4.1000
Publish_to：
Microorganisms
PMID：
39770820
Cordyceps sinensis-mediated biotransformation of notoginsenoside R1 into 25-OH-20(S/R)-R2 with elevated cardioprotective effect against DOX-induced cell injury
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Author：
Jishuang Liu, Yu Xin, Zhidong Qiu, Qi Zhang, Tianzhu He, Ye Qiu, Weinan Wang
IF：
4.0360
Publish_to：
RSC Advances
PMID：
35497008
β-Hydroxybutyrate impairs neutrophil migration distance through activation of a protein kinase C and myosin light chain 2 signaling pathway in ketotic cows
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Author：
Yuxiang Song, Yuchen Yang, Wen Zeng, Juan J. Loor, Qianming Jiang, Zhicheng Peng, Yunfei Li, Shang Jiang, Xiancheng Feng, Xiliang Du, Xinwei Li, Guowen Liu
IF：
4.0340
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
34635355
A comparative proteomic study of high and low semen quality seminal plasma in drakes
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Author：
Bincheng Tang, Guangjuan Xie, Xinyue Hu, Xin Zhang, Shenqiang Hu, Jiwei Hu, Bo Hu, Liang Li, Jiwen Wang
IF：
4.0140
Publish_to：
POULTRY SCIENCE
PMID：
36088822
Functional Characterization of the Soybean Glycine max Actin Depolymerization Factor GmADF13 for Plant Resistance to Drought Stress
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Author：
Deying Wang, Mengxue Du, Peng Lyu, Jingyu Li, Huiran Meng, Xinxin Liu, Mengmeng Shi, Yujie Gong, Qi Sha, Qingmei Men, Xiaofei Li, Yongwang Sun, Shangjing Guo
IF：
4.0000
Publish_to：
Plants-Basel
PMID：
38931083
AHR signaling pathway reshapes the metabolism of AML/MDS cells and potentially leads to cytarabine resistance
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Author：
Jia Yan, Guo Juan, Zhao Youshan, Zhang Zheng, Shi Lei, Fang Ying, Wu Dong, Wu Lingyun, Chang Chunkang
IF：
3.8480
Publish_to：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
PMID：
33709099
Single-cell sequencing reveals cellular heterogeneity of nucleus pulposus in intervertebral disc degeneration
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Author：
Jia Shu, Liu Hongmei, Yang Tao, Gao Sheng, Li Dongru, Zhang Zhenyu, Zhang Zifang, Gao Xu, Liang Yanhu, Liang Xiao, Wang Yexin, Meng Chunyang
IF：
3.8000
Publish_to：
Scientific Reports
PMID：
39516278
A Promising transwell co-culture cell model for silicosis
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Author：
Jiaqi Lv, Yi Zhang, Tao Yu, Haitao Yang, Yulu Li, Jingwei Xiao, Bin Li
IF：
3.6850
Publish_to：
TOXICOLOGY IN VITRO
PMID：
35066113
LncRNA S100PBP promotes proliferation and steroid hormone synthesis of granulosa cells by sponging MiR-2285bc-BMPR2 in bovine
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Author：
Wang Kai, Cheng Ying, Ren Yongping, Xiu Haoyu, Meng Wenxiang, Guo Tong, Ma Xiaoyan, Kebreab Ermias, Wang Dong, Lyu Lihua
IF：
3.6000
Publish_to：
BIOLOGY OF REPRODUCTION
PMID：
38412119
汕-Hydroxybutyrate impairs the directionality of migrating neutrophil through inhibiting the autophagy-dependent degradation of Cdc42 and Rac1 in ketotic cows
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Author：
Yuchen Yang, Shang Jiang, Jing Yang, Xiancheng Feng, Chao Wang, Kexin Wang, Wenwen Gao, Xiliang Du, Lin Lei, Zhe Wang, Guowen Liu, Yuxiang Song, Xinwei Li
IF：
3.5000
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
37641273
Overexpression of salusin β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells
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Author：
Aohong Xu, Lei Wang, Min Luo, Huan Zhang, Meiwei Ning, Jintong Pan, Xiuqun Duan, Yuxue Wang, Xiang Liu
IF：
3.4000
Publish_to：
Molecular Medicine Reports
PMID：
38063230
Establishment and characterization of an immortalized bovine intestinal epithelial cell line
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Author：
Meng Sudan, Wang Y uexin, Wang Shuai, Qian Weifeng, Shao Qi, Dou Mengying, Zhao Shujuan, Wang Jianguo, Li Mengyun, An Yongsheng, He Lei, Zhang Cai
IF：
3.3000
Publish_to：
JOURNAL OF ANIMAL SCIENCE
PMID：
37351870
Indocyanine Green Reduces the Viability of Human Lens Epithelial Cells and Promotes Cytolysis: An Ex Vivo Study
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Author：
Zi-Fang He, Ying-Di Zhao, Si Zhang, Fei-Fei Chen, Ya-Jun Liu, Wen-Wen Zhang, Zheng-Gao Xie
IF：
3.2830
Publish_to：
Translational Vision Science & Technology
PMID：
34817575
In vitro screening of synbiotics composed of Lactobacillus and oligosaccharides and evaluating the potential anti-allergic ability
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Author：
Jing Bai, Yuanyuan Ni, Qian Zeng, Zhihua Wu, Xin Li, Ping Tong, Hongbing Chen, Anshu Yang, Xiaoyu Cheng
IF：
3.2000
Publish_to：
JOURNAL OF FOOD SCIENCE
PMID：
40052484
Molecular and functional characterization of the retinol-binding protein 4 (RBP4) in hepatocytes of Schizothorax prenanti in response to palmitic acid
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Author：
Wang Yan, Zhu Peng, Ni Jiahui, Mo Qilang, Luo Wei, Du Zongjun, Jiang Jun, Yang Song, Zhao Liulan, Gong Quan
IF：
3.0140
Publish_to：
FISH PHYSIOLOGY AND BIOCHEMISTRY
PMID：
35230587
Knockdown of TRIM9 attenuates irinotecan‑induced intestinal mucositis in IEC‑6 cells by regulating DUSP6 expression via the P38 pathway
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Author：
Wenjun Zhao, Qingming Wang
IF：
2.9520
Publish_to：
Molecular Medicine Reports
PMID：
34676875
Aflatoxin B1 can aggravate BALB/c mice allergy to ovalbumin through changing their Th2 cells immune responses
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Author：
Yujue Deng, Hongbing Chen, Yong Wu, Jin Yuan, Qiang Shi, Ping Tong, Jinyan Gao
IF：
2.8000
Publish_to：
TOXICON
PMID：
37062343
Baicalin enhances the thermotolerance of mouse blastocysts by activating the ERK1/2 signaling pathway and preventing mitochondrial dysfunction
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Author：
Huatao Li, Xia Cong, Junxia Sui, Zhongling Jiang, Kaiqiang Fu, Yanjun Huan, Rongfeng Cao, Wenru Tian, Yanni Feng
IF：
2.7400
Publish_to：
THERIOGENOLOGY
PMID：
34808561
Chlorantraniliprole Enhances Cellular Immunity in Larvae of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)
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Author：
Qingyan Liu, Xiaoyue Deng, Liuhong Wang, Wenqi Xie, Huilai Zhang, Qing Li, Qunfang Yang, Chunxian Jiang
IF：
2.7000
Publish_to：
Insects
PMID：
39194791
Bee venom prompts the inhibition of gefitinib on proliferation, migration, and invasion of non-small cell lung cancer cells via EGFR-mediated autophagy
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Author：
Shihui Xie, Shuo Han, Jietao Gong, Zhiyuan Feng, Yang Sun, Hong Yao, Peiying Shi
IF：
2.6000
Publish_to：
TOXICON
PMID：
39442568
Mitochondrial protein isoleucyl-tRNA synthetase 2 in tumor cells as a potential therapeutic target for cervical cancer
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Author：
Xiaojiao Meng, Bo Gao, Ning Li
IF：
2.5000
Publish_to：
CytoJournal
PMID：
38989294
A375 melanoma-derived lactate controls A375 melanoma phenotypes by inducing macrophage M2 polarization via TCA cycle and TGF-β signaling
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Author：
Qifei Wang, Yurui Shi, Zelian Qin, Mengli Xu, Jingyi Wang, Yuhao Lu, Zhenmin Zhao, Hongsen Bi
IF：
2.3000
Publish_to：
PeerJ
PMID：
39995996
Acetyl l-carnitine protects adipose-derived stem cells against serum-starvation: regulation on the network composed of reactive oxygen species, autophagy, apoptosis and senescence
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Author：
Pan Tianyun, Qian Yao, Li Tian, Zhang Zikai, He Yucang, Wang Jingping, Li Liqun, Hu Yun, Lin Ming
IF：
2.0400
Publish_to：
CYTOTECHNOLOGY
PMID：
35185289
Overexpression of TAF4B Promoted the Proliferation of Undifferentiated Spermatogonia in Cattleyak In Vitro
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Author：
Yuan Tian, Peng Zhang, Kemin Jing, Yuqian Li, Binglin Yue, Zhijuan Wu, Wenjing Dong, Jincheng Zhong, Xin Cai
IF：
1.6000
Publish_to：
REPRODUCTION IN DOMESTIC ANIMALS
PMID：
39494734
技术问答
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注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
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怎么染植物叶片2025-03-05 15:05:51
您好，植物样本染色建议使用G4808-植物组织染色试剂盒（台盼蓝法），具体步骤可参考说明书
这个0.4%台盼蓝染色液的溶剂是什么？2025-02-11 10:47:56
您好，主溶剂是蒸馏水
0.4%是多大浓度的2024-09-19 08:36:11
您好，0.4%一般为质量浓度，摩尔浓度在4mM左右
说明书里面写如果有沉淀可以过滤，请问怎么过滤2024-09-04 16:32:29
您好，可以使用0.45um水系滤膜过滤
台盼蓝染色之后最多可以放多久进行测试?2023-10-24 11:05:21
您好，染色3min就可以，也可以更长一些，但不宜超过10min，长时间放置会出现假阳性，建议尽快观察。