中文
英语
产品简介
| 中文名称 | 阿尔玛蓝试剂 |
| 英文名称 | Alamar Blue Cell Viability Assay Reagent |
| 储存条件 | 2-8℃，避光保存，有效期1年。 |
| 规格 | 1ml（100T) 5ml（500T) |
| 单位 | 瓶 |
阿尔玛蓝试剂为细胞增殖和细胞毒性检测提供了一种简便、快速、可靠、安全的方法，适用于高通量检测实验。该检测试剂的主要成分是一种氧化还原指示剂。其在氧化状态下呈现紫蓝色无荧光性，而在还原状态下，转变为呈粉红或红色荧光的还原产物，吸收峰为530-560nm，而散射峰为590nm。
在细胞增殖过程中，细胞内NADPH/NADP、 FADH/FAD、 FMNH/FMN 和NADH/NAD 的比值升高，处于还原环境。摄入细胞内的染料被这些代谢中间体及细胞色素类还原后释放到细胞外并溶于培养基中，使培养基从无荧光的靛青蓝变成有荧光的粉红色。最后用普通分光光度计或荧光光度计进行检测，吸光度和荧光强度与活性细胞数成正比。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
不同密度接种得细胞在同样的反应条件下呈现不同的颜色
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技术问答
（6）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
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这个试剂在移除之后，细胞还可以继续保持正常的培养吗?2023-11-09 13:45:13
您好，可以继续正常培养用于实验测试，但是反应产物具有微弱的光毒性，不建议长期培养或传代保存。
在您给出的实验图中，有不同的颜色，但是没有标明相应的细胞浓度，请问一下，每个孔的细胞浓度是多少呢？或者说您能不能给出您参考的具体文献是哪一篇呢?2023-08-29 08:48:04
您好，这个标曲受细胞种类、活力和反应时间影响，须客户使用实验细胞系自行测试计算标曲，通常设定浓度范围在100-20000/96孔。
可以使用紫外分光光度来检测Alamar blue吗?2023-07-22 19:03:00
您好，可以的。
如何计算?2023-05-31 17:31:27
您好，建议查看官网说明书描述的计算方式。
1ml做九十六孔板可以做多少个孔啊?2023-05-06 10:21:13
您好，约100孔。