中文
英语
细胞自噬染色检测试剂盒(MDC法)
英文名称:Autophagy/Cytotoxicity Dual Stain Kit(MDC Method)
储存条件:-20℃，避光保存，有效期1年。
产品简介
| 中文名称 | 细胞自噬染色检测试剂盒(MDC法) |
| 英文名称 | Autophagy/Cytotoxicity Dual Stain Kit(MDC Method) |
| 储存条件 | -20℃，避光保存，有效期1年。 |
| 规格 | 100T |
| 单位 | 盒 |
自噬(autophagy)是细胞受到刺激后吞噬自身的细胞质或细胞器，最终将吞噬物在溶酶体内降解的过程，自噬体(autophagosome)为双层膜包被的圆形或椭圆形结构，内含细胞质、长寿蛋白质和异常蛋白聚集物，损伤或多余细胞器如线粒体、粗面内质网和微体、病毒和细菌等。
单丹磺酰尸胺(Dansylcadaverine,MDC )是一种荧光色素，是嗜酸性染色剂，通常被用于检测自噬体形成的特异性标记染色剂，其检测激发滤光片波长355nm。阻断滤光片波长512nm。细胞自噬染色检测试剂盒(MDC法)，适用于培养细胞的自噬染色，又称为MDC染色液，可与EB合用双染。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
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Author：
WenTao Ye, MinLi Dai, DanDan Bian, QingYu Zhu, Xin Li, HaiNa Sun, FanChi Li, Jing Wei, Bing Li
IF：
3.4240
Publish_to：
INSECT MOLECULAR BIOLOGY
PMID：
36093732
Corydaline alleviates Parkinson’s disease by regulating autophagy and GSK-3β phosphorylation
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Author：
Zhou Kaikai, Xu Shasha
IF：
3.4000
Publish_to：
PSYCHOPHARMACOLOGY
PMID：
38289512
Exosomes derived from bone marrow mesenchymal stem cells induce the proliferation and osteogenic differentiation and regulate the inflammatory state in osteomyelitis in vitro model
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Author：
Liang Wei, Li Yangui, Ji Yihua, Kang Renjie, Zhang Kaixi, Su Xueyuan, Li Jiangbo, Ji Mingming, Wu Taiyong, Cao Xinjie, Chen Jianrui, Huo Jianzhong
IF：
3.1000
Publish_to：
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
PMID：
39168906
PDZK1-Interacting Protein 1(PDZKIP1) Inhibits Goat Subcutaneous Preadipocyte Differentiation through Promoting Autophagy
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Author：
Dingshuang Chen, Yanyan Li, Tingting Hu, Chengsi Gong, Guangyu Lu, Xiaotong Ma, Yong Wang, Youli Wang, Yaqiu Lin
IF：
3.0000
Publish_to：
Animals
PMID：
36978587
MHY1485 promotes adriamycin sensitivity in HepG2 cells by inhibiting autophagy
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Author：
Guo Jingfeng, Lei Yingying, Liu Liwei, Wen Zhenzhen, Zhang Bo, Fang Jincun, Liang Guohui, Guo Qikun, Peng Jing
IF：
2.9000
Publish_to：
FUNCTIONAL & INTEGRATIVE GENOMICS
PMID：
38306005
Shenxian-Shengmai Oral Liquid Evoke Autophagy of Fibroblast to Attenuate Sinoatrial Node Fibrosis in Sick Sinus Syndrome Mice via the AKT/mTOR Pathway
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Author：
Chen Chen, Heng Zhang, Siyi Hou, Yue Liu, Lu Ren, Miao Hao, Keyan Chen, Lingkang Li, Xintong Cai, Zisu Deng, Zhuang Liu, Ping Hou
IF：
2.6500
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
36212944
Zingiberensis Newsaponin Inhibits the Malignant Progression of Hepatocellular Carcinoma via Suppressing Autophagy Moderated by the AKR1C1-Mediated JAK2/STAT3 Pathway
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Author：
Keqing He, Xing Liu, Shiping Cheng, Pingsheng Zhou
IF：
2.6300
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
34938341
The W9 peptide inhibits osteoclastogenesis and osteoclast activity by downregulating osteoclast autophagy and promoting osteoclast apoptosis
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Author：
Kou Yuying, Li Congshan, Yang Panpan, Li Dongfang, Lu Xiong, Liu Hongrui, Li Minqi
IF：
2.6110
Publish_to：
JOURNAL OF MOLECULAR HISTOLOGY
PMID：
34664129
Dioscin Impedes Proliferation, Metastasis and Enhances Autophagy of Gastric Cancer Cells via Regulating the USP8/TGM2 Pathway
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Author：
Ma Ting, Ge Xinguo, Zhu Jie, Song Chengxin, Wang Pinhao, Cai Jiali
IF：
2.6000
Publish_to：
MOLECULAR BIOTECHNOLOGY
PMID：
38085503
Inhibition of PI3K/Akt/mTOR pathway by ammonium chloride induced apoptosis and autophagy in MAC-T cell
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Author：
Luping Feng, Hang Liao, Jingsong Liu, Chunmei Xu, Kai Zhong, Heshui Zhu, Shuang Guo, Yujie Guo, Liqiang Han, Heping Li, Yueying Wang
IF：
2.5340
Publish_to：
RESEARCH IN VETERINARY SCIENCE
PMID：
33930632
Total Flavonoids of Engelhardia roxburghiana Wall. Leaves Alleviated Foam Cells Formation through AKT/mTOR-Mediated Autophagy in the Progression of Atherosclerosis
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Author：
Jie Wei, Liangliang Huang, Dongmei Li, Junhui He, Yanjing Li, Fei He, Weirong Fang, Guining Wei
IF：
2.4080
Publish_to：
CHEMISTRY & BIODIVERSITY
PMID：
34259387
ATG7-mediated autophagy involves in miR-138-5p regulated self-renewal and invasion of lung cancer stem-like cells derived from A549 cells
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Author：
Zhou Qian, Cui Fenghe, Lei Chenggang, Ma Shengwei, Huang Jiangping, Wang Xianghui, Qian Haiyun, Zhang Deming, Yang Yalun
IF：
2.2600
Publish_to：
ANTI-CANCER DRUGS
PMID：
33323682
Baculovirus-mediated endostatin and angiostatin activation of autophagy through the AMPK/AKT/mTOR pathway inhibits angiogenesis in hepatocellular carcinoma
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Author：
Tingting Wei, Jiajie Cheng, Yonggan Ji, Xue Cao, Shuqin Ding, Quanxia Liu, Zhisheng Wang
IF：
1.7000
Publish_to：
Open Life Sciences
PMID：
39091624
IL-27 attenuated macrophage injury and inflammation induced by Mycobacterium tuberculosis by activating autophagy
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Author：
Zhou Yushan, Zhang Yuxuan, Li Yanli, Liu Liqiong, Zhuang Min, Xiao Yi
IF：
1.5000
Publish_to：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
PMID：
39455490
技术问答
（19）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
京公网安备 11011202000391号
100T可以做几次2025-03-25 19:51:22
您好，对于悬浮细胞，每次使用10ul，可以用100次；对于贴壁细胞，96孔板可染色100次，24孔板可以染色25次，6孔板可以染色10次
您好，请问清洁液配1X的用什么稀释。2025-01-03 16:42:12
您好，用蒸馏水或双蒸水稀释即可
您好，请问可以用MDC检测细胞酸碱性吗？2024-12-23 13:10:05
您好，MDC主要用于检测细胞自噬，建议用专门的pH检测方法
您好，请问可以用在烟草上吗？2024-10-11 15:31:12
您好，这款试剂主要用于培养细胞检测，可能不太适用于烟草样本。
您好，请问该试剂盒是否可用于流式细胞术检测2024-08-06 16:05:09
您好，MDC 染色试剂盒理论上可用于流式细胞术检测自噬体，但需满足以下关键条件：1. 流式细胞仪具备紫外 / 紫光激光或 MDC 可被现有激光有效激发；2. 优化染色流程（如固定透化步骤、染色浓度和时间），降低非特异性信号；3. 实验目的为群体水平的半定量分析，而非单细胞高精度检测。