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DPPH自由基清除能力检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:DPPH Free Radical Scavenging Capacity Assay Kit
储存条件:Store at 2-8℃，1 year
产品简介
| 自备试剂 | 该试剂盒实验过程中需自备试剂，详情见网站说明书 |
| 中文名称 | DPPH自由基清除能力检测试剂盒 |
| 英文名称 | DPPH Free Radical Scavenging Capacity Assay Kit |
| 检测方法 | 微量法 Spectrophotometer/Microplate Reader |
| 储存条件 | Store at 2-8℃，1 year |
| 规格 | 100T/48S 200T/96S |
| 单位 | 盒 |
产品内容：
提取液：液体80 mL×1瓶，4℃保存；
试剂一：无水乙醇30 mL×1瓶，自备，室温保存；
试剂二：粉剂×1支（0.6 mL EP管放于8 mL试剂瓶中），4℃保存。临用前加入4.05 mL试剂一振荡溶解，用不完的试剂可于-20℃保存一月，建议分装保存；临用前根据试验所需量按照试剂二：试剂一（V:V）= 4：21的比例配制成工作液，现配现用，用不完的工作液可于4℃保存一周；
试剂三：粉剂×1支，10 mg维生素C，4℃避光保存；临用前加入1 mL提取液，充分振荡溶解；配成10 mg/mL的维生素C溶液，用于阳性对照。
产品说明：
DPPH自由基一种很稳定的氮中心的自由基，是样本抗氧化能力的重要指标之一，广泛应用于抗氧化类食品、保健品及药品的研究中。
DPPH自由基有单电子，其醇溶液呈紫色，在515 nm处有强吸收。当有抗氧化剂存在时，DPPH自由基被清除，其溶液颜色变浅，515 nm的吸光度下降，在一定范围内其吸光度的变化与自由基被清除的程度成正比。本试剂盒中，通过吸光度下降的程度来反映样本清除DPPH自由基的能力。
自备实验用品：
恒温水浴锅、可见分光光度计/酶标仪、微量玻璃比色皿/96孔板、台式离心机、无水乙醇、研钵/粉碎机、烘干箱、30~50目筛和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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JOURNAL OF FOOD SCIENCE
PMID：
37751083
Changes in amino acids, catechins and alkaloids during the storage of oolong tea and their relationship with antibacterial effect
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Author：
Cui Jilai, Wu Bin, Zhou Jie
IF：
3.8000
Publish_to：
Scientific Reports
PMID：
38710752
Effect of GABA combined with ultrasound stress germination treatment on phenolic content and antioxidant activity of highland barley
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Author：
Yanan Liu, Yingying Wu, Yuanqiang Jia, Feiyue Ren, Sumei Zhou
IF：
3.3000
Publish_to：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
38979987
Integrating network pharmacology and experimental verification to explore the pharmacological mechanisms of phlorizin against osteoarthritis
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Author：
Yao Yi, Zhong Qiuling, Zhong Yanping, Gao Zixin, Zhou Bo, Lu Chun, Zheng Li, Yin Feiying, Tan Manli
IF：
3.1000
Publish_to：
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
PMID：
39085510
Effects of electron beam irradiation on microbial load and physicochemical qualities of Ligusticum chuanxiong Hort
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Author：
Min Huang, Yi Fu, Jiang He, Xin Jia, Yan Wang, Yalu Qiu, Jian Zhao, Pan Xu, Yu Zeng, Peng Gao, Hao Chen, Mianxue Liu, Qian Chen
IF：
2.9000
Publish_to：
RADIATION PHYSICS AND CHEMISTRY
PMID：
Effects of dietary chitosan oligosaccharides supplementation on meat quality,chemical composition and anti-oxidant capacity in frizzled chickens
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Author：
Ruixia Lan, Xiaochun Chen, Yuhua Zhang, Huiwen Luo
IF：
2.5000
Publish_to：
Italian Journal of Animal Science
PMID：
Water-soluble intracellular extract of Desmodesmus sp. YT enhanced the antioxidant capacity of human skin fibroblast to protect the skin from UV damage
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Author：
Ming Ying, Zuye Zeng, Qin Li, Xianglan Chen, Ying Xiong, Bo Wu, Liang Peng, Qian Zhang, Li Wang, Zhongming Dai, Shuangfei Li, Huirong Chen, Xuewei Yang
IF：
2.3000
Publish_to：
Journal of Cosmetic Dermatology
PMID：
38327116
Polyketide production in a mangrove-associated fungus Diaporthe goulteri, induced by chemical epigenetic modification
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Author：
Xiaolin Ma, Xia Wei, Shangping Xing, Xue Cheng, Junfei Lu, Chunxia Yang, Genshi Zhao, Ziteng Dai, Shiyu Hou, Yanying Liu, Dan Zhu
IF：
1.9000
Publish_to：
NATURAL PRODUCT RESEARCH
PMID：
39714031
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