羟自由基清除能力检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Micro Hydroxyl Free Radical Scavenging Capacity Assay Kit
储存条件:Store at 2-8℃,6 months


产品简介
中文名称 | 羟自由基清除能力检测试剂盒 |
英文名称 | Micro Hydroxyl Free Radical Scavenging Capacity Assay Kit |
别名 | 羟自由基清除能力试剂盒 羟自由基清除能力测试盒 |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at 2-8℃,6 months |
运输条件 | 冷藏运输 |
规格 | 100T/96S |
单位 | 盒 |
测定意义:羟自由基作用于体内蛋白质、核酸、脂类等生物分子,造成细胞结构和功能受损,进而导致体内代谢紊乱引起疾病。羟自由基清除能力是样品抗氧化能力的重要指标之一,在抗氧化类保健品和药品研究中得到广泛应用。
测定原理:H2O2/ Fe2+ 通过Fenton反应产生羟自由基,将邻二氮菲- Fe2+水溶液中Fe2+氧化为Fe3+ ,导致536nm吸光度下降,样品对536nm吸光度下降速率的抑制程度,反映了样品清除羟自由基的能力。
需要的仪器,耗材:恒温水浴锅、可见分光光度计/酶标仪、微量玻璃比色皿/96孔板和蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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试剂盒中DPPH溶液的浓度是多少?2024-10-10 19:25:35
您好,配方保密,无法告知
你好,索莱宝fenton试剂盒的提取液成分是什么,可以用于其他无特殊要求的组织匀浆检测吗,比如说我要做组织MDA的检测,可以用这个提取液进行组织的匀浆吗?2022-08-07 14:49:55
您好,不能用于做组织DNA检测,本身不具备对样品的裂解能力,提取的过程是物理破碎的方式,提取液都是一些缓冲成分和稳定剂,如果提核酸或者提蛋白等要用对应的裂解液而不能用试剂盒中的提取液替代