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Tris 三(羟甲基)氨基甲烷
CAS:No.77-86-1
分子式:C4H11NO3
分子量:121.14
储存条件:RT，有效期7年
纯度:≥99.5%
英文名称:Tris (Hydroxymethyl) Aminomethane
产品简介
| 别名 | 三甲醇氨基甲烷；缓血酸铵；2-氨基-2-(羟甲基)-1,3-丙二醇；Aminomethyl idinetrimethanol；Talatrol；THAM；TRIS。 |
| 英文名称 | Tris (Hydroxymethyl) Aminomethane |
| CAS | No.77-86-1 |
| 分子式 | C4H11NO3 |
| 分子量 | 121.14 |
| 储存条件 | RT，有效期7年 |
| 外观(性状) |
白色至浅黄色结晶粉末
|
| 级别 | Ultra Pure Grade |
| 纯度 | ≥99.5% |
| 溶解性 | 121mg/mL in water |
| 规格 | 100g 500g 1kg 5kg |
| 单位 | 瓶 |
Tris 是一种有机化合物， 白色至微黄色结晶性粉末或块，化学式C4H11NO3，溶于乙醇和水，微溶于乙酸乙酯、苯，不溶于乙醚、四氯化碳。25°C时溶解度（mg/mL）：水550、乙二醇79.1、甲醇26、无水乙醇14.6、95%乙醇22.0、N，N-二甲基甲酰胺14，戊醇2.6，丙醇2.0，乙酸乙酯0.5、环己烷0.1。水溶液不吸收二氧化碳，对铜、铝有腐蚀作用，有刺激性。是核酸和蛋白质的溶剂，广泛应用于生物化学和分子生物学实验中缓冲液的制备。
Tris缓冲能力：
Tris为弱碱，在25°C下，它的pKa为8.1；根据缓冲理论，Tris缓冲液的有效缓冲范围在pH7.0到9.2之间。0.1mol/l的水溶液pH为10.4，一般加入盐酸以调节pH值，即可获得所需pH值的缓冲液。但同时应注意温度对于Tris的pKa的影响对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。。
Tris主要应用为：
1. 1M Tris-HCl 6.8、1.5M Tris-HCl 8.8和5×Tris-甘氨酸电泳缓冲液是SDS-PAGE最常用的试剂。
2. 在Tris盐酸缓冲液中加入EDTA制成“TE缓冲液”，TE缓冲液被用于DNA的稳定和储存。将调节pH值的盐酸换为乙酸即可以得到“TAE（Tris/Acetate/EDTA）缓冲液”，换成硼酸则获得“TBE（Tris/Borate/EDTA）缓冲液”。TAE和TBE缓冲液常用于核酸电泳实验中。
3. 其他的应用：Tris被用于不同pH条件下的蛋白质晶体生长。Tris缓冲液的低离子强度特点可用于线虫（C. elegans）核纤层蛋白（lamin）的中间纤维的形成。
贮存条件：室温干燥保存。
使用注意事项：若吸入、摄入、皮肤吸收，对身体有害，使用时注意防护。
Tris缓冲液不仅被广泛用作核酸和蛋白质的溶剂，还有许多重要用途。Tris被用于不同pH条件下的蛋白质晶体生长。Tris缓冲液的低离子强度特点可用于线虫（C. elegans）核纤层蛋白（lamin）的中间纤维的形成。Tris也是蛋白质电泳缓冲液的主要成分之一，其在电泳缓冲液中与甘氨酸构成缓冲体系，稳定电泳过程中的PH。此外，Tris还是制备表面活性剂、硫化促进剂和一些药物的中间物。Tris也被用作滴定标准物。 作为蛋白缓冲液时，若后续工作需要质谱，则不适宜用Tris，最好换成其他质谱仪可耐受的缓冲液。
常用于配置缓冲液：
T1010 1.5M Tris-HCL (pH8.8)
T1020 1M Tris-HCL (pH6.8)
T1090 1M Tris-HCL(pH=7.4)
T1130 1M Tris-HCl(pH=7.0)
T1140 1M Tris-HCl(pH=7.5)
T1150 1M Tris-HCl(pH=8.0)
T1160 1M Tris-HCl(pH=9.0)
T1050 5×TBE 缓冲液
T1051 10×TBE 缓冲液
T1060 50×TAE 缓冲液
T1070 5×Tris-甘氨酸电泳缓冲液
T1510 10x Tris-mops 缓冲液
T1510 10x Tris-mops 缓冲液
T1120 TE缓冲液，pH=8.0
备注：产品信息可能会有优化升级。请以实际标签信息为准。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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BIOMEDICINE & PHARMACOTHERAPY
PMID：
39094544
Theoretical insights into the mechanism underlying aflatoxin B1 transformation by the BsCotA-methyl syringate system
点击查看 >>
Author：
Xiaolu Wang, Lin Cui, Mengting Liu, Zheng Qi, Huiying Luo, Huoqing Huang, Tao Tu, Xing Qin, Yuan Wang, Jie Zhang, Yaru Wang, Bin Yao, Yingguo Bai, Xiaoyun Su
IF：
6.8000
Publish_to：
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
PMID：
38301584
Quantified Instant Conjugation of Peptides on a Nanogold Surface for Tunable Ice Recrystallization Inhibition
点击查看 >>
Author：
Shixuan Yang, Zhongxiang Ding, Leiming Chu, Mengke Su, Honglin Liu
IF：
6.7000
Publish_to：
Nanoscale
PMID：
38047706
Dioscin alleviates myocardial infarction injury via regulating BMP4/NOX1-mediated oxidative stress and inflammation
点击查看 >>
Author：
Zhe Zhang, Xuerong Zhao, Meng Gao, Lina Xu, Yan Qi, Jinhong Wang, Lianhong Yin
IF：
6.6560
Publish_to：
PHYTOMEDICINE
PMID：
35675750
Genistein-3′-sodium sulfonate Attenuates Neuroinflammation in Stroke Rats by Down-Regulating Microglial M1 Polarization through α7nAChR-NF-κB Signaling Pathway
点击查看 >>
Author：
Chaoming Liu, Song Liu, Lijiao Xiong, Limei Zhang, Xiao Li, Xingling Cao, Jinhua Xue, Liangdong Li, Cheng Huang, Zhihua Huang
IF：
6.5820
Publish_to：
International Journal of Biological Sciences
PMID：
33867831
Sensitive electrochemical detection of oxytetracycline based on target triggered CHA and poly adenine assisted probe immobilization
点击查看 >>
Author：
Weihang Wang, Chi Zhang, Jiaxin Guo, Gaiping Li, Baoxian Ye, Lina Zou
IF：
6.5580
Publish_to：
ANALYTICA CHIMICA ACTA
PMID：
34556208
Zi Shen Decoction Inhibits Growth and Metastasis of Lung Cancer via Regulating the AKT/GSK-3β/β-Catenin Pathway
点击查看 >>
Author：
Yanxia Ma, Yu Liu, Linxin Teng, En Luo, Dekang Liu, Fuqiong Zhou, Kaiyuan Wang, Weiping Chen, Lei Bi
IF：
6.5430
Publish_to：
Oxidative Medicine and Cellular Longevity
PMID：
33777320
Stimulus-responsive and dual-target DNA nanodrugs for rheumatoid arthritis treatment
点击查看 >>
Author：
Yi Zhang, Jiaqi Wang, Rongrong Luo, Fanfan Guo, Xiaonan Wang, Xiao Chu, Yongxing Zhao, Pengchao Sun
IF：
6.5100
Publish_to：
INTERNATIONAL JOURNAL OF PHARMACEUTICS
PMID：
36572263
Dicyclohexylcarbodiimide and disodium succinate induce the change of energy metabolism in relation to longan pulp breakdown
点击查看 >>
Author：
Yi Zheng, Hetong Lin, Yueying Sang, Mengshi Lin, Yixiong Lin, Hui Wang, Zhongqi Fan, Yihui Chen, Yifen Lin
IF：
6.5000
Publish_to：
Food Chemistry-X
PMID：
39100246
Usage of nanocrystalline cellulose as a novel cryoprotective substance for the Nemipterus virgatus surimi during frozen storage
点击查看 >>
Author：
Zhengyi Li, Qi Wang, Shuting Li, Yu Chang, Xuan Zheng, Hui Cao, Yafeng Zheng
IF：
6.4430
Publish_to：
Food Chemistry-X
PMID：
36404893
Peptidoglycan-Chi3l1 interaction shapes gut microbiota in intestinal mucus layer
点击查看 >>
Author：
Yan Chen, Ruizhi Yang, Bin Qi, Zhao Shan
IF：
6.4000
Publish_to：
eLife
PMID：
39373714
Selenium nanoparticles in aquaculture: Unique advantages in the production of Se-enriched grass carp (Ctenopharyngodon idella)
点击查看 >>
Author：
Chao Zhu, Zifang Wu, Qimin Liu, Xiaolin Wang, Lijuan Zheng, Shuyang He, Fangxia Yang, Hong Ji, Wuzi Dong
IF：
6.3000
Publish_to：
Animal Nutrition
PMID：
38357572
Fabrication and characterization of core-shell gliadin/tremella polysaccharide nanoparticles for curcumin delivery: Encapsulation efficiency, physicochemical stability and bioaccessibility
点击查看 >>
Author：
Xiaomin Zhang, Zihao Wei, Xin Wang, Yuming Wang, Qingjuan Tang, Qingrong Huang, Changhu Xue
IF：
6.2690
Publish_to：
Current Research in Food Science
PMID：
36561330
Amplification-free microRNA profiling with femtomolar sensitivity on a plasmonic enhanced fluorescence nano-chip
点击查看 >>
Author：
Ruibin Hu, Yiyi Liu, Guanghui Wang, Jiahui Lv, Jingkai Yang, Hongjun Xiao, Ying Liu, Bo Zhang
IF：
6.2000
Publish_to：
ANALYTICA CHIMICA ACTA
PMID：
37858557
Identification of lysine-lactylated substrates in gastric cancer cells
点击查看 >>
Author：
Dawei Yang, Jie Yin, Liuqun Shan, Xingling Yi, Wei Zhang, Yongbin Ding
IF：
6.1070
Publish_to：
iScience
PMID：
35800753
Controlling the amount of coupling agents on the synthesis of coating antigens to enhance the sensitivity of fluoroquinolone immunodetection.
点击查看 >>
Author：
Jinpeng Zhu, Jinfeng Dou, Caie Wu, Gongjian Fan, Tingting Li, Dongbei Shen
IF：
6.1000
Publish_to：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
37341110
Pipeline for precise insoluble matrisome coverage in tissue extracellular matrices
点击查看 >>
Author：
Wei Chen, Wen Zhang, Ning Zhang, Shuyan Chen, Tao Huang, Hong You
IF：
6.0640
Publish_to：
Frontiers in Bioengineering and Biotechnology
PMID：
37284237
The construct of triple responsive nanocomposite and its antibacterial effect
点击查看 >>
Author：
Guize Li, Luohuizi Li, Zhiqiang Wang, Siqing Zhong, Mingkang Li, Hongwei Wang, Lin Yuan
IF：
5.9990
Publish_to：
COLLOIDS AND SURFACES B-BIOINTERFACES
PMID：
35121427
Effects of Pereskia aculeate Miller Petroleum Ether Extract on Complete Freund’s Adjuvant-Induced Rheumatoid Arthritis in Rats and its Potential Molecular Mechanisms
点击查看 >>
Author：
Chen Yifei, Liu Kaifei, Qin Yingyuan, Chen Suyi, Guan Guokai, Huang Yao, Chen Yu, Mo Zhixian
IF：
5.9880
Publish_to：
Frontiers in Pharmacology
PMID：
35614946
Bidentatide, a Novel Plant Peptide Derived from Achyranthes bidentata Blume: Isolation, Characterization, and Neuroprotection through Inhibition of NR2B-Containing NMDA Receptors
点击查看 >>
Author：
Fei Ding, Yunpeng Bai, Qiong Cheng, Shu Yu, Mengchun Cheng, Yulin Wu, Xiaozhe Zhang, Xinmiao Liang, Xiaosong Gu
IF：
5.9240
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
34360755
Asiatic acid inhibits angiogenesis and vascular permeability through the VEGF/VEGFR2 signaling pathway to inhibit the growth and metastasis of breast cancer in mice
点击查看 >>
Author：
Miaomiao Tian, Kan Chen, Jianhua Huang, Dongqing Chu, Jialin Li, Keqiang Huang, Chunyu Ma
IF：
5.8820
Publish_to：
PHYTOTHERAPY RESEARCH
PMID：
34541711
Red fluorescent nanoprobe based on Ag@Au nanoparticles and graphene quantum dots for H2O2 determination and living cell imaging
点击查看 >>
Author：
Shang Ling-Ling, Song Xiaojie, Niu Chang-Bin, Lv Qi-Yan, Li Chun-Ling, Cui Hui-Fang, Zhang Shoutao
IF：
5.8330
Publish_to：
MICROCHIMICA ACTA
PMID：
34363101
Precolumn derivatization LC-MS/MS method to simultaneous quantitation of 10 monosaccharides in rat plasma
点击查看 >>
Author：
Fenfen Liu, Zhuolin Dong, Mengru Li, Junhong Sun, Ziye Hou, Ayesha Younas, Xiangling Wan, Hongtao Shang, Nan Zhang
IF：
5.8000
Publish_to：
INTERNATIONAL JOURNAL OF PHARMACEUTICS
PMID：
37394158
A Selective Fluorescent l-Lactate Biosensor Based on an l-Lactate-Specific Transcription Regulator and Förster Resonance Energy Transfer
点击查看 >>
Author：
Xianzhi Xu, Rong Xu, Shuang Hou, Zhaoqi Kang, Chuanjuan Lü, Qian Wang, Wen Zhang, Xia Wang, Ping Xu, Chao Gao, Cuiqing Ma
IF：
5.7430
Publish_to：
Biosensors-Basel
PMID：
36551077
Mn2+-modified black phosphorus nanosensor for detection of exosomal microRNAs and exosomes
点击查看 >>
Author：
Xia Qing, Zheng Jintao, Bu Jianlan, Li Rui, Li Xinxin, Fan Shuting, Ling Kai, Jiang Hongyan
IF：
5.7000
Publish_to：
MICROCHIMICA ACTA
PMID：
37458810
Insight into the Neuroprotective Effect of Genistein-3′-Sodium Sulfonate Against Neonatal Hypoxic-Ischaemic Brain Injury in Rats by Bioinformatics
点击查看 >>
Author：
Xie Ting, Shuang Liyan, Liu Gaigai, Zhao Shanshan, Yuan Zhidong, Cai Hao, Jiang Lixia, Huang Zhihua
IF：
5.6820
Publish_to：
MOLECULAR NEUROBIOLOGY
PMID：
36370154
Target-driven functionalized DNA hydrogel capillary sensor for SARS-CoV-2 dual-mode detection
点击查看 >>
Author：
Jing-bo Jiao, Qing Kang, Shu-xin Cui, Jiang-li Cao, Tong Lin, Chen-jing Ma, Ze-hui Xiao, Ting Du, Nan Wang, Xin-jun Du, Shuo Wang
IF：
5.6000
Publish_to：
TALANTA
PMID：
39644672
LC-MS/MS Insight into Vitamin C Restoration to Metabolic Disorder Evoked by Amyloid β in Caenorhabditis elegans CL2006
点击查看 >>
Author：
Simeng Zhang, Yuchan Deng, Annan Zhang, Lili Yan, Zhichao Zhang, Jing Wei, Qiang Zhang
IF：
5.5810
Publish_to：
Metabolites
PMID：
36144245
Betulinic acid mitigates zearalenone-induced liver injury by ERS/MAPK/Nrf2 signaling pathways in mice
点击查看 >>
Author：
Jing Wu, Jiayan Li, You Wu, Mengran Yang, Yunqin Chen, Naidong Wang, Ji Wang, Zhihang Yuan, Jine Yi, Chenglin Yang
IF：
5.5720
Publish_to：
FOOD AND CHEMICAL TOXICOLOGY
PMID：
37179046
The Change in the Structure and Functionality of Ferritin during the Production of Pea Seed Milk
点击查看 >>
Author：
Yilin Xing, Jiaqi Ma, Qimeng Yao, Xuemin Chen, Jiachen Zang, Guanghua Zhao
IF：
5.5610
Publish_to：
Foods
PMID：
35206035
An “on-off-on” fluorescence probe for glyphosate detection based on Cu2+ modulated g-C3N4 nanosheets
点击查看 >>
Author：
Qin Yingfeng, Huang Ruiqi, Ye Gao-Jie
IF：
5.5450
Publish_to：
Frontiers in Chemistry
PMID：
36247672
Assessing B-Z DNA Transitions in Solutions via Infrared Spectroscopy
点击查看 >>
Author：
Mengmeng Duan, Yalin Li, Fengqiu Zhang, Qing Huang
IF：
5.5000
Publish_to：
Biomolecules
PMID：
37371544
The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway
点击查看 >>
Author：
Meng-Qi Zhang, Ke-Xin Sun, Xu Guo, Ying-Ying Chen, Cai-Yun Feng, Jia-Shu Chen, Joao C.M. Barreira, Miguel A. Prieto, Jin-Yue Sun, Jian-Dong Zhang, Ning-Yang Li, Chao Liu
IF：
5.4000
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
37308029
Drug-guided screening for pancreatic lipase inhibitors in functional foods
点击查看 >>
Author：
Lujia Zhang, Jinkai Zheng, Mingzhe Ma, Yue Zhao, Jia Song, Xin Chen, Wanxiu Cao, Xiao He, Changhu Xue, Qingjuan Tang
IF：
5.3960
Publish_to：
Food & Function
PMID：
33912875
An antigen/chemotherapy co-loaded DNA nanocube inserts into tumor cell plasma membrane and enhances chemo- and immunotherapy
点击查看 >>
Author：
Jun Cao, Junhong Sun, Yuzhen Zhang, Zhuolin Dong, Mengru Li, Fenfen Liu, Ayesha Younas, Nan Zhang, Yibing Chen
IF：
5.3000
Publish_to：
INTERNATIONAL JOURNAL OF PHARMACEUTICS
PMID：
39662859
Kallistatin prevents ovarian hyperstimulation syndrome by regulating vascular leakage
点击查看 >>
Author：
Jianfang Huang, Yuling Mao, Quanxin Li, Honghai Hong, Ni Tang, Xiangjin Kang, Yuling Huang, Jianqiao Liu, Qing Gong, Yachao Yao, Lei Li
IF：
5.2950
Publish_to：
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
PMID：
35866203
Hierarchically hybrid biocoatings on Ti implants for enhanced antibacterial activity and osteogenesis
点击查看 >>
Author：
Zheng Wang, Li Mei, Xinqiang Liu, Qihui Zhou
IF：
5.2680
Publish_to：
COLLOIDS AND SURFACES B-BIOINTERFACES
PMID：
33964526
Fabrication of Non-phospholipid Liposomal Nanocarrier for Sustained-Release of the Fungicide Cymoxanil
点击查看 >>
Author：
Zhang Zheng, Yang Jun, Yang Qing, Tian Guangyong, Cui Zhong-Kai
IF：
5.2460
Publish_to：
Frontiers in Molecular Biosciences
PMID：
33859996
A sensitive fluorescence biosensor based on metal ion-mediated DNAzyme activity for amplified detection of acetylcholinesterase
点击查看 >>
Author：
Xu-Hua Zhao, Xiao-Chun Dai, Ya-Nan Zhou, Han-Xiao Zhang, Xiao-Hua Cui, Xiang Zhai, Bao-Feng Yu, Zhi-Ling Song
IF：
5.2270
Publish_to：
ANALYST
PMID：
35579472
Effect of High-Hydrostatic-Pressure Treatment on the Physicochemical Properties of Kafirin
点击查看 >>
Author：
Yajing Yang, Qiongling Chen, Qingshan Liu, Xiaowen Wang, Wenbin Bai, Zhenjia Chen
IF：
5.2000
Publish_to：
Foods
PMID：
38002135
Synergy of “Yiqi” and “Huoxue” components of QishenYiqi formula in ischemic stroke protection via lysosomal/inflammatory mechanisms
点击查看 >>
Author：
Yule Wang, Xinyan Liu, Wen Zhang, Shuang He, Yiqian Zhang, John Orgah, Yi Wang, Yan Zhu
IF：
5.1950
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
35436536
Study on lipid nanomicelles targeting placenta for the treatment of preeclampsia
点击查看 >>
Author：
Yang Liu, Qimeng Zhang, Xingli Gao, Tong Wang
IF：
5.0160
Publish_to：
JOURNAL OF DRUG TARGETING
PMID：
35502921
Rapid FRET Assay for the Early Detection of Alpha-Synuclein Aggregation in Parkinson’s Disease
点击查看 >>
Author：
Hang Yu, Rui Feng, Fenglin Chen, Zuodong Wu, Dehai Li, Xue Qiu
IF：
5.0000
Publish_to：
ACS Chemical Neuroscience
PMID：
38506367
Design and Evaluation of Complex Polypeptide-Loaded Dissolving Microneedles for Improving Facial Wrinkles in Different Areas
点击查看 >>
Author：
Mengzhen Xing, Han Liu, Fanda Meng, Yuning Ma, Suohui Zhang, Yunhua Gao
IF：
4.9670
Publish_to：
Polymers
PMID：
36365468
Dioscin ameliorates diabetes cognitive dysfunction via adjusting P2X7R/NLRP3 signal
点击查看 >>
Author：
Zhi Lu, Yiqun Yao, Jinhong Wang, J.-Y. Peng
IF：
4.9320
Publish_to：
INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：
34785142
In Vivo Detection of Tetrodotoxin in Takifugu obscurus Based on Solid-Phase Microextraction Coupled with Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry
点击查看 >>
Author：
Hengli Meng, Shui Jiang, Yin Zhang, Yun Hu, Yuan Liu
IF：
4.9270
Publish_to：
MOLECULES
PMID：
36144854
Discovery and evaluation of active compounds from Xuanfei Baidu formula against COVID-19 via SARS-CoV-2 Mpro
点击查看 >>
Author：
Zhang Min, Liu Liting, Zhao Yao, Cao Yipeng, Zhu Yan, Han Lifeng, Yang Qi, Wang Yu, Wang Changjian, Zhang Han, Wang Yuefei, Zhang Junhua
IF：
4.9000
Publish_to：
Chinese Medicine
PMID：
37528477
Indole-3-acetic acid improves the hepatic mitochondrial respiration defects by PGC1a up-regulation
点击查看 >>
Author：
Chen Zhang, Qingsong Fu, Kai Shao, Limin Liu, Xiaotian Ma, Fengyi Zhang, Xiaodong Zhang, Liying Meng, ChuanZhu Yan, Xiaoyun Zhao
IF：
4.8500
Publish_to：
CELLULAR SIGNALLING
PMID：
35988807
Horizontal comparison of “red or blue shift” and binding energy of six fluoroquinolones: Fluorescence quenching mechanism, theoretical calculation and molecular modeling method
点击查看 >>
Author：
Haonan Lu, Zishan Li, Yongshan Zhou, Hao Jiang, Yongfeng Liu, Changchun Hao
IF：
4.8310
Publish_to：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
PMID：
35597157
P-hydroxybenzaldehyde protects Caenorhabditis elegans from oxidative stress and β-amyloid toxicity
点击查看 >>
Author：
Yu Xingzhi, Tao Jie, Xiao Tian, Duan Xiaohua
IF：
4.8000
Publish_to：
Frontiers in Aging Neuroscience
PMID：
38841104
PSMA5 contributes to progression of lung adenocarcinoma in association with the JAK/STAT pathway
点击查看 >>
Author：
Lu Feng, Zhou Jing, Chen Qing, Zhu Jianling, Zheng Xiaowei, Fang Na, Qiao Ling
IF：
4.7410
Publish_to：
CARCINOGENESIS
PMID：
35605971
Comprehensive analysis of the prognostic and role in immune cell infiltration of MSR1 expression in lower-grade gliomas
点击查看 >>
Author：
Qiankun Ji, Kai Huang, Yuan Jiang, Kunjian Lei, Zewei Tu, Haitao Luo, Xingen Zhu
IF：
4.7110
Publish_to：
Cancer Medicine
PMID：
35142109
Electrospun Fiber Membrane with the Dual Affinity of Chelation and Covalent Interactions for the Efficient Enrichment of Glycoproteins
点击查看 >>
Author：
Yaqi Liu, Xing Lu, Shiling Liu, Yijun Li, Xiwen He, Langxing Chen, Yukui Zhang
IF：
4.7000
Publish_to：
ACS Applied Bio Materials
PMID：
38517141
All-in-one in situ colorimetric RT-LAMP assay for point-of-care testing of SARS-CoV-2
点击查看 >>
Author：
Yugan He, Liqin Wang, Xiaoping An, Yigang Tong
IF：
4.6160
Publish_to：
ANALYST
PMID：
34505858
Heparin purification by expanded bed anion exchange in a countercurrent chromatography column
点击查看 >>
Author：
Kun Cai, Jie Chen, Yang Liu, Bilal Muhammad Khan, Yongjie Zhao
IF：
4.6010
Publish_to：
JOURNAL OF CHROMATOGRAPHY A
PMID：
36075135
Effects of Ultra-High-Pressure Jet Processing on Casein Structure and Curdling Properties of Skimmed Bovine Milk
点击查看 >>
Author：
Fei Xu, Lu Xue, Yanfeng Ma, Tianjiao Niu, Pei Zhao, Zijian Wu, Yanfa Wang
IF：
4.6000
Publish_to：
MOLECULES
PMID：
36903641
Protective effect of Huangpu Tongqiao capsule against Alzheimer's disease through inhibiting the apoptosis pathway mediated by endoplasmic reticulum stress in vitro and in vivo
点击查看 >>
Author：
Xinquan Li, Yunpeng Qin, Shu Ye, Hang Song, Peng Zhou, Biao Cai, Yan Wang
IF：
4.5620
Publish_to：
SAUDI PHARMACEUTICAL JOURNAL
PMID：
36465852
A novel electrochemical platform for assay of alkaline phosphatase based on amifostine and ATRP signal amplification
点击查看 >>
Author：
Zhang Yaping, Li Peipei, Lu Jing, Li Daoxiang, Yang Huaixia, Li Xiaofei, Liu Yanju
IF：
4.4780
Publish_to：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
PMID：
35972525
The Role of Acetylation and Methylation of Rat Hippocampal Histone H3 in the Mechanism of Aluminum-Induced Neurotoxicity
点击查看 >>
Author：
Gao Jie, Liu Wei, Liu Jiaqi, Hao Niping, Pei Jing, Zhang Lifeng
IF：
4.4000
Publish_to：
NEUROCHEMICAL RESEARCH
PMID：
37897558
An Enhanced Lateral Flow Assay Based on Aptamer–Magnetic Separation and Multifold AuNPs for Ultrasensitive Detection of Salmonella Typhimurium in Milk
点击查看 >>
Author：
Pingping Gao, Lihan Wang, Yang He, Yitian Wang, Xinyan Yang, Shiqian Fu, Xue Qin, Qing Chen, Chaoxin Man, Yujun Jiang
IF：
4.3500
Publish_to：
Foods
PMID：
34359475
Biochemical characterization of a new ulvan lyase and its applicability in utilization of ulvan and preparation of ulva oligosaccharides
点击查看 >>
Author：
Li Chen, Tang Tiancheng, Jiang Jinju, Yao Zhong, Zhu Benwei
IF：
4.3000
Publish_to：
GLYCOBIOLOGY
PMID：
37593920
Nimbolide attenuated the inflammation in the liver of autoimmune hepatitis's mice through regulation of HDAC3
点击查看 >>
Author：
Dingchao Xia, Dazhi Chen, Tingchen Cai, Lujian Zhu, Yanhan Lin, Sijie Yu, Kailu Zhu, Xiaodong Wang, Lanman Xu, Yongping Chen
IF：
4.2190
Publish_to：
TOXICOLOGY AND APPLIED PHARMACOLOGY
PMID：
34780724
Highly Resistant and Sensitive Colorimetric Immunochromatographic Assay for Sibutraminem (SBT) Illegally Adulterated into Diet Food based on PDA/AuNPs Labelling
点击查看 >>
Author：
Yun Zhao, Sijie Huang, Mengjia chao, Yulong Wang, Pengyan Liu, Pan Li, Xuechen Fang, Michael N Routledge, Chifang Peng, Cunzheng Zhang
IF：
4.2000
Publish_to：
ANALYST
PMID：
37671915
Electrochemical detection of alkaline phosphatase activity through enzyme-catalyzed reaction using aminoferrocene as an electroactive probe
点击查看 >>
Author：
Wang Wenbin, Lu Jing, Hao Lulu, Yang Huaixia, Song Xuejie, Si Fuchun
IF：
4.1570
Publish_to：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
PMID：
33481047
Siderophore-synthesizing NRPS reprogram lipid metabolic profiles for phenotype and function changes of Arthrobotrys oligospora
点击查看 >>
Author：
Liu Huiwen, Sun Liangyin, Zhang Jintao, Wang Yongzhong, Lu Hengqian
IF：
4.1000
Publish_to：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
PMID：
38114752
Polyethyleneimine-assisted co-deposition of polydopamine coating with enhanced stability and efficient secondary modification
点击查看 >>
Author：
Chun-gong Li, Qinqin Yang, Dong Chen, Hongliang Zhu, Jiachen Chen, Runjin Liu, Qi Dang, Xiang Wang
IF：
4.0360
Publish_to：
RSC Advances
PMID：
36540211
Study on the mechanism of enhanced gel strength of heat-induced egg white by shikimic acid braising
点击查看 >>
Author：
Hui Xue, Meng Xu, Guowen Zhang, Ping Wang, Lin Yu, Ying Zhao, Yonggang Tu, Yan Zhao
IF：
4.0140
Publish_to：
POULTRY SCIENCE
PMID：
35278755
Water-Soluble Se-Containing Proteins from Chicken Alleviate DSS-Induced Ulcerative Colitis in Mice via Inhibiting TLR4/MyD88 Pathway and Protecting the Goblet Cell Pathway
点击查看 >>
Author：
Li Yuancheng, Xu Yanlong, Hu Xin, Li Chenxi, Wei Lulu, Wang Xinlei, Jin Yi, Hu Zhongqiu
IF：
3.9000
Publish_to：
BIOLOGICAL TRACE ELEMENT RESEARCH
PMID：
37950138
Upconverting phosphor technology-based lateral flow assay for the rapid and sensitive detection of anti-Trichinella spiralis IgG antibodies in pig serum
点击查看 >>
Author：
Li Jian, Ding Jing, Liu Xiao-Lei, Tang Bin, Bai Xue, Wang Yang, Qiao Wei-Dong, Liu Ming-Yuan, Wang Xue-Lin
IF：
3.8760
Publish_to：
Parasites & Vectors
PMID：
34551787
Vitamin B6 prevents Isocarbophos-induced posterior cerebral artery injury in offspring rats through up-regulating S1P receptor expression
点击查看 >>
Author：
Liu Yanhua, Yang Kunli, Wang Ling, Yang Jinfang, Wang Yang, Luo Hu, Li Peng, Yin Yaling
IF：
3.8480
Publish_to：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
PMID：
34718372
Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons
点击查看 >>
Author：
Zhiyuan Wu, Yongjie Zhang, Yuyuan Liu, Xuemei Chen, Zhiwen Huang, Xiaoming Zhao, Hongyun He, Yihao Deng
IF：
3.8170
Publish_to：
Life-Basel
PMID：
34575099
Preparation, characterization, and anticancer effects of an inclusion complex of coixol with β-cyclodextrin polymers
点击查看 >>
Author：
Xing-Chen Wang, Xin-Yu Shen, Lin Chen, Rong Wei, Ming-Yuan Wei, Cai-Hong Gu, Rong-Rong Xu, Sheng-Qing Ding, Bo Pan
IF：
3.8000
Publish_to：
PHARMACEUTICAL BIOLOGY
PMID：
38126136
Targeted Screening of Curcumin Derivatives as Pancreatic Lipase Inhibitors Using Computer-Aided Drug Design
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Author：
Yuxuan Jing, Laichun Luo, Zhaoxiang Zeng, Xueyan Zhao, Rongzeng Huang, Chengwu Song, Guiying Chen, Sha Wei, Haijun Yang, Yinping Tang, Shuna Jin
IF：
3.7000
Publish_to：
ACS Omega
PMID：
38947805
Effects of temperature and pH on the structure of a metalloprotease from Lactobacillus fermentum R6 isolated from Harbin dry sausages and molecular docking between protease and meat protein
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Author：
Fangda Sun, Ran Tao, Qian Liu, Hui Wang, Baohua Kong
IF：
3.6390
Publish_to：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
33548144
Tendon-regulating and bone-setting manipulation promotes the recovery of synovial inflammation in rabbits with knee osteoarthritis via the TLR4–MyD88–NF-κB signaling pathway
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Author：
Xiangyu Jin, Yangyang Yu, Yongyan Lin, Jinping Yang, Zhaohui Chen
IF：
3.6160
Publish_to：
Annals of Translational Medicine
PMID：
37082688
Pancreatic lipase and alpha-glucosidase inhibitors screening from Schisandra chinensis based on spectrum-effect relationship and ultra-high-performance liquid chromatography-tandem mass spectrometry
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Author：
Fu-Qin He, Guang-Zhen Wan, Juan Chen
IF：
3.6140
Publish_to：
JOURNAL OF SEPARATION SCIENCE
PMID：
36189874
Dopamine-polyethyleneimine co-deposition of a capillary for α-glucosidase immobilization and its application in enzyme inhibitor screening
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Author：
Peng Li, Fu-Qin He, Xiao-Hui Ma, Ling Jin, Juan Chen
IF：
3.5350
Publish_to：
ELECTROPHORESIS
PMID：
34347304
Synthesis of PEGylated cationic curdlan derivatives with enhanced biocompatibility
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Author：
Muqier Muqier, Hai Xiao, Xiang Yu, Yifeng Li, Mingming Bao, Qingming Bao, Shuqin Han, Huricha Baigude
IF：
3.5170
Publish_to：
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
PMID：
34641765
Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection
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Author：
Qiuzi Zhao, Ping Du, Xiaoyong Wang, Mengqian Huang, Ling-Dong Sun, Tao Wang, Zhiyun Wang
IF：
3.5120
Publish_to：
ACS Omega
PMID：
34151102
Proteomic approach-based comparison of metabolic pathways and functional activities of whey proteins derived from Guishan and Saanen goat milk
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Author：
Qiong Zhao, Kunlin Li, Kexin Jiang, Ziyou Yuan, Menglin Xiao, Guangqiang Wei, Wentao Zheng, Xuefeng Wang, Aixiang Huang
IF：
3.5000
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
36870847
Disruption of Dense Granular Protein 2 (GRA2) Decreases the Virulence of Neospora caninum
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Author：
Jingquan Dong, Nan Zhang, Panpan Zhao, Jianhua Li, Lili Cao, Xiaocen Wang, Xin Li, Ju Yang, Xichen Zhang, Pengtao Gong
IF：
3.4120
Publish_to：
Frontiers in Veterinary Science
PMID：
33681332
circGLI3 Inhibits Oxidative Stress by Regulating the miR-339-5p/VEGFA Axis in IPEC-J2 Cells
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Author：
Zhi-xin Li, Li-xue Wang, Yu Zhang, Wei Chen, Yong-qing Zeng
IF：
3.4110
Publish_to：
Biomed Research International
PMID：
34423029
Colorectal cancer cell membrane biomimetic ferroferric oxide nanomaterials for homologous bio-imaging and chemotherapy application
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Author：
Li Jun, Lin Chenyu, Zhu Yuqian, Shao Chengwei, Wang Tiegong, Chen Bingdi
IF：
3.4000
Publish_to：
MEDICAL ONCOLOGY
PMID：
37801170
Dopamine-polyethyleneimine co-deposition cellulose filter paper for α-Glucosidase immobilization and enzyme inhibitor screening
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Author：
Peng Li, Xiao-Hui Ma, Ling Jin, Juan Chen
IF：
3.2050
Publish_to：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
PMID：
33621797
Effects of the covalent conjugation between caffeic acid and peanut allergen protein Ara h1 on the antigenicity and structure of Ara h1
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Author：
Na Wang, Qingqing Liu, Qilei Shi, Fan Wang, Chao Xu, Hongtao Ren, Qiuying Yu
IF：
3.2000
Publish_to：
JOURNAL OF FOOD SCIENCE
PMID：
39150685
Effects of stewing with tea polyphenol on the gel properties, microstructure, and secondary structure of boiled egg white
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Author：
Hui Xue, Meng Xu, Guowen Zhang, Feng Feng, Yuting Wang, Danhui Cao, Yonggang Tu, Yan Zhao
IF：
3.1670
Publish_to：
JOURNAL OF FOOD SCIENCE
PMID：
34564852
Construction of a novel fluorescent DNA aptasensor for the fast-response and sensitive detection of copper ions in industrial sewage
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Author：
Yue Mou, Yanfei Zhang, Xinru Lin, Meiyun Chen, Yuxiang Xia, Shu Zhu, Chonghui Wei, Xingyu Luo
IF：
3.1000
Publish_to：
Analytical Methods
PMID：
37431579
Cardiotoxicity of patulin was found in H9c2 cells
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Author：
Baigang Zhang, Hairong Liang, Ke Huang, Jinliang Li, Dongmei Xu, Chenghui Huang, Yi Li
IF：
3.0330
Publish_to：
TOXICON
PMID：
34929212
An integrated method for IgG N-glycans enrichment and analysis: Understanding the role of IgG glycosylation in diabetic foot ulcer
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Author：
Yanwei Xiao, Xuefang Dong, Cheng Chen, Yun Cui, Tongbin Chu, Xiuling Li, Aoxue Wang
IF：
3.0000
Publish_to：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
PMID：
38163392
Heat-induced antigen retrieval utilizing modified Tris-EDTA buffer for reprobing of Western blots on nitrocellulose paper
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Author：
Jun-Ling Wang, Ze-Ting Xu, Ling Zhan, Min Liao, Chao-Jin Xu
IF：
2.9000
Publish_to：
ANALYTICAL BIOCHEMISTRY
PMID：
37150424
Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of Osteoarthritis
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Author：
Guang-yao Chen, Xiao-yu Liu, Jing Luo, Xin-bo Yu, Yi Liu, Qing-wen Tao
IF：
2.8090
Publish_to：
Computational and Mathematical Methods in Medicine
PMID：
35720033
Effect and mechanism of Fisetin on myocardial damage induced by Patulin
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Author：
Xu Dongmei, Zhang Baigang, Huang Chenghui, Lu Jiao, Li Yang, Fu Binggang
IF：
2.8000
Publish_to：
MOLECULAR BIOLOGY REPORTS
PMID：
37341918
Purification and structure analysis of zinc-binding protein from Mizuhopecten yessoensis
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Author：
Chunying Meng, Kuiwu Wang, Xiaojun Zhang, Xinyue Zhu
IF：
2.7200
Publish_to：
JOURNAL OF FOOD BIOCHEMISTRY
PMID：
33993503
Ethyl acetate extract of Gastrodia elata protects Caenorhabditis elegans from oxidative stress and amyloid β peptide toxicity
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Author：
Xiongfei Shi, Xingzhi Yu, Liping Yang, Xiaohua Duan
IF：
2.7000
Publish_to：
Experimental and Therapeutic Medicine
PMID：
37522064
Spermine oxidase regulates liver inflammation and fibrosis through β-catenin pathway
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Author：
Tingting Hu, Wenqing Tang, Wandong Hong, Qingke Huang, Xuecheng Sun, Wenzhi Wu, Jie Zhang
IF：
2.6000
Publish_to：
Clinics and Research in Hepatology and Gastroenterology
PMID：
39002816
Preparation, characterization and in vitro antioxidant activities of a homogeneous polysaccharide from Prunella vulgaris
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Author：
Jiaqi Huang, Mengyao Wang, Yu Wang, He Sun, Yadan Zou, Xiaojin Tian, Dianxin Cui, Peng Zhang, Xue Li, Wenzhi Yang
IF：
2.5000
Publish_to：
FITOTERAPIA
PMID：
39725087
Comparison of the Nutritional Composition of Bullfrog Meat from
Different Parts of the Animal
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Author：
Yanli Zhu, Min Bao, Chong Chen, Xiaoli Yang, Wenliang Yan, Fazheng Ren, Pengjie Wang, Pengcheng Wen
IF：
2.4710
Publish_to：
Food Science of Animal Resources
PMID：
34796330
miRNA-383-5p Regulated Migration and Invasion of Tumor Cells by Inhibiting NCKAP1 Expression in Gastric Cancer
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Author：
Wang Chen, Wang Pan, Tian Yuan, Lu Cuijuan, Liu Lixia, Wu Jianguo, Wang Yanan, Li Jinghua
IF：
2.4000
Publish_to：
BIOCHEMICAL GENETICS
PMID：
38625593
Protective effect of Gastrodia elata Blume in a Caenorhabditis elegans model of Alzheimer's disease based on network pharmacology
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Author：
Xiongfei Shi, Yuan Luo, Liping Yang, Xiaohua Duan
IF：
2.3000
Publish_to：
Biomedical Reports
PMID：
37113386
Combined addition of L-carnitine and L-proline improves cryopreservation of dairy goat semen
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Author：
Jianglin Zhao, Peng Meng, Miaomiao Jin, Xianghai Ma, Hongwei Ma, Hanwen Yang, Yanzhi Chen, Junyu Zhang, Yong Zhang, Yan Luo, Jun Liu
IF：
2.2000
Publish_to：
ANIMAL REPRODUCTION SCIENCE
PMID：
37677888
Effects of Altitude and Duration of Differing Levels of Hypoxic Exposure on Hypoxia-Inducible Factor-1α in Rat Tissues
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Author：
Xiao-lin Li, Wei-gang Wang, Mao-xing Li, Tian-long Liu, Xiu-yu Tian, Lan Wu
IF：
2.1830
Publish_to：
HIGH ALTITUDE MEDICINE & BIOLOGY
PMID：
35708531
The interaction between neutrophils and atrial myocytes in the occurrence and development of atrial fibrillation
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Author：
Li Tao, Wang Yan, Wan Jie, Duan Yu, Xu Yang, Yang Mao
IF：
2.0000
Publish_to：
BMC Cardiovascular Disorders
PMID：
39333891
A simple colorimetric method based on “on–off–on” mode for detection of H2S and Hg2+ in water
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Author：
Su Lixia, Zhang Xue, Su Yonghuan, Liu Bingqian
IF：
1.9670
Publish_to：
ANALYTICAL SCIENCES
PMID：
35974229
Screening and characterization of cosmetic efficacy components of Terminalia chebula based on biological activity-guided methodology
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Author：
Cai-Cai Liang, Feng-Qin Zhang, Juan Chen
IF：
1.8000
Publish_to：
BIOMEDICAL CHROMATOGRAPHY
PMID：
39090681
Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata
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Author：
Chunying Meng, Kuiwu Wang, Xiaojun Zhang, Xinyue Zhu
IF：
1.6500
Publish_to：
PROTEIN EXPRESSION AND PURIFICATION
PMID：
33561519
Protocol for tissue-specific mutagenesis with fluorescent labeling in zebrafish
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Author：
Juanjuan Luo, Chunjiao Lu, Xiaojun Yang
IF：
1.3000
Publish_to：
STAR Protocols
PMID：
39058589
Effects of Netrin-1 and NHE1 Participation on the Migration of Macrophages Driven by CCL2
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Author：
Shiyue Zhang, Xiangang Mo, Shuping Zhang, Juhai Chen, Lan Wang, Hui Yang
IF：
1.2060
Publish_to：
CELLULAR AND MOLECULAR BIOLOGY
PMID：
36227668
Rapid Separation and Display of Active Fibrinogenolytic Agents in Sipunculus nudus through Fibrinogen-Polyacrylamide Gel Electrophoresis
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Author：
Bin Kang, Chengjia Hu, Hongjun Lin, Hui Yan, Chengyun Wei, Mingqing Tang
IF：
1.2000
Publish_to：
Jove-Journal of Visualized Experiments
PMID：
38709073
Protocol to Retrieve Unknown Flanking DNA Using Fork PCR for Genome Walking
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Author：
Hongjing Wu, Hao Pan, Haixing Li
IF：
1.0000
Publish_to：
Bio-protocol
PMID：
39872713
Cycloheximide (CHX) Chase Assay to Examine Protein Half-life
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Author：
Ying Miao, Qian Du, Hong-Guang Zhang, Yukang Yuan, Yibo Zuo, Hui Zheng
IF：
0.8000
Publish_to：
Bio-protocol
PMID：
37323633
技术问答
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注意：
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1M Tris-HCL (pH6.8)怎么配制?2023-09-21 11:33:47
您好，用水溶解盐酸调PH。
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