| 英文名称 |
Anti-DYRK3 Polyclonal Antibody |
| 中文名称 |
双特异性酪氨酸-(Y)-磷酸化调节激酶3抗体 |
| 宿主 |
Rabbit |
| 克隆类型 |
Polyclonal Antibody |
| 亚型 |
IgG |
| 预测交叉反应 |
Monkey |
| 稀释比例 |
WB 1:2000-5000. IHC 1:50-200. |
| 靶标 |
DYRK3 |
| 别名 |
Dual specificity tyrosine (Y) phosphorylation regulated kinase 3; Dual specificity tyrosine phosphorylation-regulated kinase 3; Dual specificity tyrosine-phosphorylation-regulated kinase 3; Dyrk3; DYRK3_HUMAN; DYRK5; hYAK3 2; Protein kinase Dyrk3; RED; REDK; Regulatory erythroid kinase; |
| 背景说明 |
Dual-specificity protein kinase that promotes disassembly of several types of membraneless organelles during mitosis, such as stress granules, nuclear speckles and pericentriolar material (PubMed:29973724). Dual-specificity tyrosine-regulated kinases (DYRKs) autophosphorylate a critical tyrosine residue in their activation loop and phosphorylate their substrate on serine and threonine residues (PubMed:9748265, PubMed:29634919). Acts as a central dissolvase of membraneless organelles during the G2-to-M transition, after the nuclear-envelope breakdown: acts by mediating phosphorylation of multiple serine and threonine residues in unstructured domains of proteins, such as SRRM1 and PCM1 (PubMed:29973724). Does not mediate disassembly of all membraneless organelles: disassembly of P-body and nucleolus is not regulated by DYRK3 (PubMed:29973724). Dissolution of membraneless organelles at the onset of mitosis is also required to release mitotic regulators, such as ZNF207, from liquid-unmixed organelles where they are sequestered and keep them dissolved during mitosis (PubMed:29973724). Regulates mTORC1 by mediating the dissolution of stress granules: during stressful conditions, DYRK3 partitions from the cytosol to the stress granule, together with mTORC1 components, which prevents mTORC1 signaling (PubMed:23415227). When stress signals are gone, the kinase activity of DYRK3 is required for the dissolution of stress granule and mTORC1 relocation to the cytosol: acts by mediating the phosphorylation of the mTORC1 inhibitor AKT1S1, allowing full reactivation of mTORC1 signaling (PubMed:23415227). Also acts as a negative regulator of EPO-dependent erythropoiesis: may place an upper limit on red cell production during stress erythropoiesis (PubMed:10779429). Inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells (PubMed:10779429). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1: this in turn inhibits p53/TP53 activity and apoptosis (PubMed:20167603). |
| Swiss Prot |
O43781 |
| Gene ID |
8444 |
| 亚细胞定位 |
Cytoplasm Nucleus |
| 免疫原 |
Recombinant protein of human DYRK3 |
| Public Immunogen Range |
24-370aa |
| 理论分子量 |
64/66kDa |
| 实际分子量 |
66kDa |
| 纯化方法 |
Affinity purification |
| 储存液 |
PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| 储存条件 |
Store at -20℃. Avoid freeze / thaw cycles. |
| 规格 |
20ul 50ul 100ul |
| 交叉反应 |
Human Mouse Rat |
| 应用 |
WB IHC |
| 研究领域 |
干细胞 细胞生物学 发育生物学 信号传导 表观遗传 |
| 单位 |
支 |
| 属性分类 |
其他抗体 |
中文
英语
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