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糖原PAS染色试剂盒(细胞专用)
英文名称:Glycogen Periodic Acid Schiff(PAS) Stain Kit(For Cells)
储存条件:2-8℃，避光保存，有效期6个月。
产品简介
| 中文名称 | 糖原PAS染色试剂盒(细胞专用) |
| 英文名称 | Glycogen Periodic Acid Schiff(PAS) Stain Kit(For Cells) |
| 储存条件 | 2-8℃，避光保存，有效期6个月。 |
| 货期 | 2-3天 |
| 规格 | 5*20ml 5*50ml |
| 单位 | 盒 |
糖原染色是病理学中常规的染色方法之一，McManus在1946年最先使用高碘酸-雪夫技术显示黏蛋白，该法常用来显示糖原和其他多糖，该技术不仅能显示糖原，还能显示中性黏液性物质和某些酸性物质以及软骨、垂体、霉菌、真菌、色素、淀粉样物质、基底膜等。过碘酸(又称高碘酸)是一种强氧化剂，它能氧化糖类及有关物质中的1，2-乙二醇基，使之变为二醛，醛与Schiff试剂能结合成一种品红化合物，产生紫红色。由于高碘酸还可氧化细胞内其他物质，使用时应注意选择好高碘酸浓度和氧化时间，使氧化控制在既能把乙二醇基氧化成醛基又不至于过氧化，这是很关键的步骤。
糖原PAS染色试剂盒(细胞专用)性能稳定，特异性强；操作简捷，仅需1h; 氧化剂、苏木素浓度更低，更适用于细胞、超薄组织切片染色；无盐酸乙醇分化步骤。该试剂盒仅适用于科研领域。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
培养Hela细胞
细胞核着紫蓝色，糖原和糖蛋白着粉红色
细胞核着紫蓝色，糖原和糖蛋白着粉红色
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Yangfan Hou, Shuping Zheng, Fan Zou, Dan Wang, Hongju Da, Yong Zhou, Xinping Fan, Jianghao Liu, Hongyan Zhao, Jin He, Hongxin Li, Xiuzhen Sun, Yun Liu
IF：
2.8000
Publish_to：
IMMUNOBIOLOGY
PMID：
37515878
Isoleucine Enhanced the Function of the Small Intestinal Mucosal Barrier in Weaned Piglets to Alleviate Rotavirus Infection
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Author：
Rongkun Zhao, Changsheng Jiang, Yuchen Yuan, Shen Zhang, Ahmed H. Ghonaim, Chuanyan Che, Xiaojin Li, Mengmeng Jin, Erhui Jin, Xiangfang Zeng, Shenghe Li, Man Ren
IF：
2.7000
Publish_to：
Animals
PMID：
39518871
Ticagrelor Protects against Sepsis-Induced Acute Kidney Injury through an Adenosine Receptor-Dependent Pathway
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Author：
Cao Yu-han, Xu Qian-cheng, Wang Yu-wei, Ling Yang, Fu Cong
IF：
2.6400
Publish_to：
Current Medical Science
PMID：
35678913
Mutations in GCK May Lead to MODY2 by Reducing Glycogen Synthesis
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Author：
Zongyue Li, Kunxia Li, Yan Sun, Xiuyun Jiang, Jia Liu, Jingyi Li, Li Fang, Guimei Li, Qingbo Guan, Chao Xu
IF：
2.6000
Publish_to：
Advanced Biology
PMID：
35770790
Polysaccharides from Tremella Fuciformis Enhance Glucose and Lipid Metabolism in HepG2 Cells Through Activating the AMPK Signaling Pathway
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Author：
Haizhao Song, Jing Lu, Rou Deng
IF：
2.3000
Publish_to：
CHEMISTRY & BIODIVERSITY
PMID：
39319532
Ocular surface involvement and histopathologic changes in the acute stage of Stevens-Johnson syndrome and toxic epidermal necrolysis: a cross-sectional study
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Author：
Liu Yingyi, Feng Jianing, Ren Yuerong, Shi Wen, Kang Huanmin, Peng Yingqian, Tan Yixin, Wu Ruifang, Zhang Guiying, He Yan
IF：
2.0000
Publish_to：
BMC Ophthalmology
PMID：
37400766
Role of NADPH Oxidase 4 on Dry Eye Syndrome in Mice
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Author：
Mian Guo, Taixiang Liu, Yuan Miao, Xiaoli Pan, Bo Liu
IF：
1.9000
Publish_to：
JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS
PMID：
38669123
Evaluation of ocular surface inflammation and systemic conditions in patients with systemic lupus erythematosus: a cross-sectional study
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Author：
Yuerong Ren, Jing Tian, Wen Shi, Jianing Feng, Yingyi Liu, Huanmin Kang, Yan He
IF：
1.7000
Publish_to：
BMC Ophthalmology
PMID：
39533209
Expression of USP22 and the chromosomal passenger complex is an indicator of malignant progression in oral squamous cell carcinoma
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Author：
Tian Liu Jing Liu Qiuyue Chen Shengjian Jin Sisi M
IF：
1.6640
Publish_to：
Oncology Letters Pages: 2040-2046
PMID：
30675271
技术问答
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染色后可以用荧光显微镜观察到吗2025-03-14 10:07:38
您好，PAS染色无特异性荧光，需要使用普通光学显微镜观察
细胞染色，阳性率怎么计算2024-11-12 10:15:35
您好，选择细胞数量较多且分布适中的视野，在选定视野中计算所有阳性和阴性细胞，然后用阳性细胞数量除以细胞总数进行阳性率计算
这款试剂盒里的PAS固定液，使用后细胞形态基本没有改变，固定效果也很好。请问这款固定液可不可以用于免疫荧光细胞固定，有无单品可以买，或者大概成分包含什么？2024-08-13 21:54:18
您好，PAS固定液无单品售卖，含一定比例醇类物质，该固定液对抗原保存效果较差，不建议用于免疫荧光固定细胞，建议使用免疫荧光染色专用固定液
您好，我做细胞孔板染色，48孔板需要加多少的染色剂呢?2024-03-11 18:39:46
您好，通常来说，不同规格的板子整体添加液体体积都在6ml左右，即48孔板每孔约125ul。
你好，我想问一下这个可以做体外血管拟态的PAS-CD34双染么?2022-12-26 11:37:45
您好，可以的。