糖原PAS染色试剂盒(真菌专用)
英文名称:Glycogen Periodic Acid Schiff(PAS) Stain Kit(For Fungus)
储存条件:2-8℃,避光保存,有效期6个月。
产品简介
中文名称 | 糖原PAS染色试剂盒(真菌专用) |
英文名称 | Glycogen Periodic Acid Schiff(PAS) Stain Kit(For Fungus) |
储存条件 | 2-8℃,避光保存,有效期6个月。 |
规格 | 3*20ml 3*50ml |
单位 | 盒 |
糖原染色是病理学中常规的染色方法之一,McManus在1946年最先使用高碘酸-雪夫技术显示黏蛋白,该法常用来显示糖原和其他多糖,该技术不仅能显示糖原,还能显示中性黏液性物质和某些酸性物质以及软骨、垂体、霉菌、真菌、色素、淀粉样物质、基底膜等。过碘酸(又称高碘酸)是一种强氧化剂,它能氧化糖类及有关物质中的1,2-乙二醇基,使之变为二醛,醛与Schiff试剂能结合成一种品红化合物,产生紫红色。由于高碘酸还可氧化细胞内其他物质,使用时应注意选择好高碘酸浓度和氧化时间,使氧化控制在既能把乙二醇基氧化成醛基又不至于过氧化,这是很关键的步骤。
糖原PAS染色液(真菌专用)利用真菌含有多糖,过碘酸氧化真菌壁上的多糖而暴露出醛基,醛基与无色Schiff结合呈现红色。该染色法性能稳定,特异性强;操作简捷,仅需0.5h;浓度低过碘酸更适用于对真菌染色,无盐酸乙醇分化液分化步骤。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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