中文
英语
产品简介
| 中文名称 | 钙盐染色试剂盒(Von Kossa法) |
| 英文名称 | Calcium Stain Kit(Von Kossa Method) |
| 储存条件 | 2-8℃，避光保存，有效期1年。 |
| 货期 | 2-3天 |
| 规格 | 2*50ml |
| 单位 | 盒 |
钙在人体内大量存在，构成骨骼作为支持人体的支架，在分泌、运送、肌肉收缩、神经传导等也起重要作用。钙在机体内以两种形式存在，一种是离子钙，存在血液循环内，即所谓血钙；另一种是结合钙，和蛋白、碳酸或磷酸结合而沉着在组织内。除骨骼和牙齿外，正常时钙渗透在所有组织和细胞中，一般不以固体状态出现在组织内。但在某些情况下，钙析出成固体并沉着于组织内， 则为病理性钙盐沉着。沉着的钙盐主要是磷酸钙，其次为碳酸钙。钙盐通常是单折射的，但草酸钙是双折射的。许多染料可以与钙形成螯合物，包括茜素红S、红紫素、核固红等。钙盐染色常用方法有硝酸银法和茜素红S法。
本产品采用硝酸银法，即一种金属置换法，硝酸银溶液作用于含有不溶性钙盐的切片时，钙被银所置换，银盐在光的作用下被还原为黑色金属银，适用于大量样本的钙盐组织染色。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
胎鼠石蜡切片
钙盐着红褐到黑色，背景无色或组织原色
钙盐着红褐到黑色，背景无色或组织原色
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技术问答
（8）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
京公网安备 11011202000391号
你好，血管做出来没阳性，不确定是什么原因，请问什么组织可作为阳性样本2025-03-18 09:08:07
您好，未脱钙小鼠下缘肋骨或剑突石蜡切片
您好，请问这个试剂可用于细胞钙化染色不？2025-02-14 13:19:03
您好，可以
通常胎鼠用什么组织作对照呢2024-09-11 22:15:12
您好，可用不脱钙的组织，例如肋骨作为对照
这个试剂盒是应用于石蜡硬组织切片还是塑料包埋硬组织切片?2024-01-15 16:08:39
您好，应用于不脱钙或部分脱钙样本切片，由于硬度问题一般不脱钙只能制备塑料包埋硬组织切片。如组织本身含钙量不高可尝试直接制备石蜡组织切片进行染色。
EDTA脱钙完可以用VONKOSSA或者茜素红染色来测矿化么?2023-05-30 09:43:26
您好，已脱钙组织不可以进行钙盐染色，如需检测组织矿化可以考虑不脱钙直接塑封包埋。