中文
英语
乙酰辅酶A含量检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Acetyl Co-A Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 英文名称 | Acetyl Co-A Activity Assay Kit |
| 别名 | 乙酰辅酶A试剂盒 乙酰辅酶A测试盒 |
| 检测方法 | 微量法 Spectrophotometer/Microplate Reader |
| 储存条件 | Store at -20℃，6 months |
| 推荐 | 若使用96孔板测定，需使用96孔UV板，推荐货号YA0602 |
| 规格 | 100T/96S |
| 单位 | 盒 |
测定意义：乙酰辅酶A广泛存在于动物、植物、微生物和培养细胞中。是生物体能源物质代谢过程中产生的一种重要的中间代谢产物。在体内能源物质代谢中是一个枢纽性的物质。糖、脂肪、蛋白质三大营养物质通过乙酰辅酶A汇聚成一条共同的代谢通路-三羧酸循环和氧化磷酸化，经过这条通路彻底氧化生成二氧化碳和水，释放能量用于ATP合成。此外，乙酰辅酶A是合成脂肪酸，酮体，胆固醇及其衍生物等生理活性物质的前体物质。
测定原理：苹果酸脱氢酶可催化苹果酸和NAD生成草酰乙酸和NADH。柠檬酸合酶可催化乙酰辅酶A和草酰乙酸生成柠檬酸和辅酶A。利用苹果酸脱氢酶和柠檬酸合酶的偶联反应，乙酰辅酶A含量和NADH的生成速率成正比，340nm下吸光值的上升速率反应了乙酰辅酶A含量的高低。
需要的仪器，耗材:紫外分光光度计/酶标仪、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
相关产品
暂无相关产品
相关论文
更多
Combinatorial metabolic engineering enables the efficient production of ursolic acid and oleanolic acid in Saccharomyces cerevisiae
点击查看 >>
Author：
Ke Jin, Xun Shi, Jiaheng Liu, Wenwen Yu, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Long Liu
IF：
11.4000
Publish_to：
BIORESOURCE TECHNOLOGY
PMID：
36868430
Enhancing intraneural revascularization following peripheral nerve injury through hypoxic Schwann-cell-derived exosomes: an insight into endothelial glycolysis
点击查看 >>
Author：
Sun Jun, Zeng Qiuhua, Wu Zhimin, Li Zhangyu, Gao Qun, Liao Zhi, Li Hao, Ling Cong, Chen Chuan, Wang Hui, Zhang Baoyu
IF：
10.6000
Publish_to：
JOURNAL OF NANOBIOTECHNOLOGY
PMID：
38789980
Astaxanthin overproduction of Phaffia rhodozyma PR106 under titanium dioxide stress by transcriptomics and metabolic regulation analysis
点击查看 >>
Author：
Jing Zhang, Qingru Li, Yanhong Lu, Xiaoyu Guan, Jiahuan Liu, Na Xu, Chunyu Cai, Xia Li, Bo Nan, Jingsheng Liu, Yuhua Wang
IF：
9.6420
Publish_to：
BIORESOURCE TECHNOLOGY
PMID：
34555753
Crizotinib Shows Antibacterial Activity against Gram-Positive Bacteria by Reducing ATP Production and Targeting the CTP Synthase PyrG
点击查看 >>
Author：
Yun-Dan Zheng, Tairan Zhong, Haiming Wu, Nan Li, Zuye Fang, Linlin Cao, Xing-Feng Yin, Qing-Yu He, Ruiguang Ge, Xuesong Sun
IF：
9.0430
Publish_to：
Microbiology Spectrum
PMID：
35674439
Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes
点击查看 >>
Author：
Yao Yuan, Shi Jiaming, Zhang Chunlai, Gao Wei, Huang Ning, Liu Yaobei, Yan Weiwen, Han Yingguang, Zhou Wenjuan, Kong Liang
IF：
9.0000
Publish_to：
Cell Death & Disease
PMID：
37935660
Dual PPARγ/ɑ agonist oroxyloside suppresses cell cycle progression by glycolipid metabolism switch-mediated increase of reactive oxygen species levels
点击查看 >>
Author：
Yuxin Zhou, Yongjian Guo, Yejin Zhu, Yuening Sun, Wei Li, Zhiyu Li, Libin Wei
IF：
7.3760
Publish_to：
FREE RADICAL BIOLOGY AND MEDICINE
PMID：
33713839
Danhong injection alleviates cerebral ischemia/reperfusion injury by improving intracellular energy metabolism coupling in the ischemic penumbra
点击查看 >>
Author：
Miaolin Zeng, Huifen Zhou, Yu He, Zhixiong Wang, Chongyu Shao, Junjun Yin, Haixia Du, Jiehong Yang, Haitong Wan
IF：
6.5300
Publish_to：
BIOMEDICINE & PHARMACOTHERAPY
PMID：
34058441
PP2C phosphatases Ptc1 and Ptc2 dephosphorylate PGK1 to regulate autophagy and aflatoxin synthesis in the pathogenic fungus Aspergillus flavus
点击查看 >>
Author：
Zhuo Zhu, Mingkun Yang, Guang Yang, Bei Zhang, Xiaohong Cao, Jun Yuan, Feng Ge, Shihua Wang
IF：
6.4000
Publish_to：
mBio
PMID：
37754565
TMT-Based Quantitative Proteomics and Non-targeted Metabolomic Analyses Reveal the Antibacterial Mechanism of Hexanal against Vibrio parahaemolyticus
点击查看 >>
Author：
Qiuxia Fan, Xinru Dong, Zewei Wang, Yuan Yue, Yahong Yuan, Zhouli Wang, Tianli Yue
IF：
6.1000
Publish_to：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
37498004
Efficient Synthesis of Limonene in Saccharomyces cerevisiae Using Combinatorial Metabolic Engineering Strategies
点击查看 >>
Author：
Xiao Kong, Yaokang Wu, Wenwen Yu, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Long Liu
IF：
5.8950
Publish_to：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
37189018
HIPK3 Inhibition by Exosomal hsa-miR-101-3p Is Related to Metabolic Reprogramming in Colorectal Cancer
点击查看 >>
Author：
Tao Lihuiping, Xu Changliang, Shen Weixing, Tan Jiani, Li Liu, Fan Minmin, Sun Dongdong, Lai Yueyang, Cheng Haibo
IF：
5.7380
Publish_to：
Frontiers in Oncology
PMID：
35096570
Cellulase mediated stress triggers the mutations of oleaginous yeast Trichosporon cutaneum with super-large spindle morphology and high lipid accumulation
点击查看 >>
Author：
Qi Liu, Yuanyuan Li, Weiliang Hou, Bin Zhang, Jie Bao
IF：
5.7260
Publish_to：
Biotechnology Journal
PMID：
37182226
Modular Metabolic Engineering of Saccharomyces cerevisiae for Enhanced Production of Ursolic Acid
点击查看 >>
Author：
Yuan Zhu, Xiaoguang Yan, Weiguo Li, Jianjun Qiao, Guang-Rong Zhao
IF：
5.7000
Publish_to：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
39883850
Metabolomic Effects of the Dietary Inclusion of Hermetia illucens Larva Meal in Tilapia
点击查看 >>
Author：
Bo Ye, Jian Li, Lijun Xu, Hui Liu, Manjun Yang
IF：
5.5810
Publish_to：
Metabolites
PMID：
35448473
Combining protein and metabolic engineering strategies for biosynthesis of melatonin in Escherichia coli
点击查看 >>
Author：
Zhang Yanfeng, He Yongzhi, Zhang Nan, Gan JiaJia, Zhang Shan, Dong Zhiyang
IF：
5.3280
Publish_to：
Microbial Cell Factories
PMID：
34454478
Construction and Application of an Escherichia coli Strain Lacking 62 Genes Responsible for the Biosynthesis of Enterobacterial Common Antigen and Flagella
点击查看 >>
Author：
Jun Qiao, Xin Tan, Danyang Huang, Hedan Li, Zhen Wang, Hongyu Ren, Xiaoqing Hu, Xiaoyuan Wang
IF：
5.2790
Publish_to：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
33787256
Simultaneously targeting SOAT1 and CPT1A ameliorates hepatocellular carcinoma by disrupting lipid homeostasis
点击查看 >>
Author：
Ren Meiling, Xu Huanji, Xia Hongwei, Tang Qiulin, Bi Feng
IF：
5.2410
Publish_to：
Cell Death Discovery
PMID：
34052835
Fasting induces hepatic lipid accumulation by stimulating peroxisomal dicarboxylic acid oxidation
点击查看 >>
Author：
Xiao Zhang, Ting Gao, Senwen Deng, Lin Shang, Xiaocui Chen, Kai Chen, Ping Li, Xiaojuan Cui, Jia Zeng
IF：
5.1570
Publish_to：
JOURNAL OF BIOLOGICAL CHEMISTRY
PMID：
33811861
Epileptiform Discharges Reduce Neuronal ATP Production by Inhibiting F0F1-ATP Synthase Activity via A Zinc-α2-Glycoprotein-Dependent Mechanism
点击查看 >>
Author：
Liang Yi, Zhao Lili, Dai Chengcheng, Liu Guohui, Zhong Yuke, Liu Hang, Mo Lijuan, Tan Changhong, Liu Xi, Chen Lifen
IF：
5.1000
Publish_to：
MOLECULAR NEUROBIOLOGY
PMID：
37468739
Construction of an Escherichia coli Strain Lacking Fimbriae by Deleting 64 Genes and Its Application for Efficient Production of Poly(3-Hydroxybutyrate) and l-Threonine
点击查看 >>
Author：
Jun Qiao, Xin Tan, Hongyu Ren, Zheng Wu, Xiaoqing Hu, Xiaoyuan Wang
IF：
4.7920
Publish_to：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
PMID：
33863704
Improvement of acetyl-CoA supply and glucose utilization increases l-leucine production in Corynebacterium glutamicum
点击查看 >>
Author：
Yingyu Wang, Jianzhong Xu, Zhengyu Jin, Xiaole Xia, Weiguo Zhang
IF：
4.6770
Publish_to：
Biotechnology Journal
PMID：
34870372
Comprehensive Response of Rhodosporidium kratochvilovae to Glucose Starvation: A Transcriptomics-Based Analysis
点击查看 >>
Author：
Meixia He, Rui Guo, Gongshui Chen, Chao Xiong, Xiaoxia Yang, Yunlin Wei, Yuan Chen, Jingwen Qiu, Qi Zhang
IF：
4.5000
Publish_to：
Microorganisms
PMID：
37764012
Small extracellular vesicles derived from human adipose-derived stem cells regulate energetic metabolism through the activation of YAP/TAZ pathway facilitating angiogenesis
点击查看 >>
Author：
Jun Sun, Zhengjie Lin, Zhi Liao, Zhimin Wu, Hao Li, Hui Wang
IF：
4.4730
Publish_to：
CELL BIOLOGY INTERNATIONAL
PMID：
36279478
Simultaneous enhancement of lignin-derived inhibitor tolerance and lipid accumulation of oleaginous yeast Trichosporon cutaneum by adaptive evolution
点击查看 >>
Author：
Qi Liu, Bin Zhang, Mingshan Hu, Jie Bao
IF：
4.4000
Publish_to：
PROCESS BIOCHEMISTRY
PMID：
Protocatechudehyde improves mitochondrial energy metabolism through the HIF1α/PDK1 signaling pathway to mitigate ischemic stroke-elicited internal capsule injury
点击查看 >>
Author：
Miaolin Zeng, Chongyu Shao, Huifen Zhou, Yu He, Wentao Li, Jieqiong Zeng, Xixi Zhao, Jiehong Yang, Haitong Wan
IF：
4.3600
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
34044078
Effect of glucose levels on carbon flow rate, antioxidant status, and enzyme activity of yeast during fermentation
点击查看 >>
Author：
Dongdong Xie, Yingqi Sun, Yanan Lei
IF：
4.1250
Publish_to：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
35318660
Engineering Saccharomyces cerevisiae for high-efficient production of ursolic acid via cofactor engineering and acetyl-CoA optimization
点击查看 >>
Author：
Nan Jia, Jingzhi Li, Guowei Zang, Yuan Yu, Xiaojv Jin, Yuna He, Meilin Feng, Xuemei Na, Ying Wang, Chun Li
IF：
3.9000
Publish_to：
BIOCHEMICAL ENGINEERING JOURNAL
PMID：
Engineering a Yeast Cell Factory to Sustainably Biosynthesize Parthenolide
点击查看 >>
Author：
Wenping Wei, Chengshuai Yang, Zhen Su, Yan Wang, Pingping Wang, Xing Yan, Zhihua Zhou
IF：
3.7000
Publish_to：
ACS Synthetic Biology
PMID：
40050240
Boosted Meat Flavor by the Metabolomic Effects of Nile Tilapia Dietary Inclusion of Zophobas atratus Larval Meal
点击查看 >>
Author：
Yanfeng Li, Haozheng Li, Ge Zhang, Jiale Liu, Dawa Drolma, Bo Ye, Manjun Yang
IF：
3.3000
Publish_to：
Frontiers in Bioscience-Landmark
PMID：
39614443
CD39 is induced by nutrient deprivation in colorectal cancer cells and contributes to the tolerance to hypoxia
点击查看 >>
Author：
Zhaochen Ning, Keyan Liu, Huabao Xiong
IF：
2.5000
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
38991255
技术问答
（0）
我要评论
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
京公网安备 11011202000391号