单脱氢抗坏血酸还原酶(MDHAR)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Monodehydroascorbate Reductase(MDHAR) Activity Assay Kit
储存条件:Store at -20℃,6 months


产品简介
中文名称 | 单脱氢抗坏血酸还原酶(MDHAR)活性检测试剂盒 |
英文名称 | Monodehydroascorbate Reductase(MDHAR) Activity Assay Kit |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at -20℃,6 months |
推荐 | 若使用96孔板测定,需使用96孔UV板,推荐货号YA0602 |
规格 | 100T/96S |
单位 | 盒 |
测定意义:MDHAR催化MDHA还原生成AsA,在抗坏血酸氧化还原代谢中具有重要作用。
测定原理:MDHAR 催化 NADH 还原MDHA 生成 AsA和NAD+,NADH在340 nm有特征吸收峰,但是NAD+没有。通过测定340 nm光吸收下降速率,来计算出MDHAR活性。
需要的仪器,耗材:研钵、冰、台式离心机、紫外分光光度计/酶标仪、微量石英比色皿/96孔板、可调式移液枪和双蒸水
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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