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谷氨酰胺合成酶(GS)活性检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:Glutamine Synthetase(GS) Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 谷氨酰胺合成酶(GS)活性检测试剂盒 |
| 英文名称 | Glutamine Synthetase(GS) Activity Assay Kit |
| 检测方法 | 可见分光光度法 Spectrophotometer |
| 储存条件 | Store at -20℃，6 months |
| 规格 | 50T/24S |
| 单位 | 盒 |
测定意义：GS（EC 6.3.1.2）主要存在于植物中，是生物体内氨同化的关键酶之一，催化铵离子和谷氨酸合成谷氨酰胺，不仅可以防止过多的铵离子对生物有毒性，而且谷氨酰胺也是氨的主要储存和运输形式。
测定原理：GS 在ATP 和Mg2+存在下，催化铵离子和谷氨酸合成谷氨酰胺；谷氨酰胺进一步转化为γ─谷氨酰基异羟肟酸，在酸性条件下与铁形成红色的络合物；该络合物在540nm 处有最大吸收峰，可用分光光度计测定。
所需的仪器和用品：可见分光光度计、水浴锅、台式离心机、可调式移液器、1 mL 玻璃比色皿、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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