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阿利新蓝染色试剂盒(pH=1.0)
英文名称:Alcian Blue Stain Kit，pH 1.0
CAS:No.75881-23-1
储存条件:2-8℃，避光保存，有效期6个月。
产品简介
| 中文名称 | 阿利新蓝染色试剂盒(pH=1.0) |
| 英文名称 | Alcian Blue Stain Kit，pH 1.0 |
| CAS | No.75881-23-1 |
| 储存条件 | 2-8℃，避光保存，有效期6个月。 |
| 规格 | 3*50ml |
| 单位 | 盒 |
阿利新蓝（Alcian）又称爱先蓝或阿尔辛蓝等，是一种类铜钛花青共轭染料，最初用于纺织纤维染色。这种阳离子染料与酸性基团结合，即阿尔辛蓝与组织内含有的阴离子基团如羧基和硫酸根形成不溶性复合物。阿利新蓝由中央含铜的酞菁环与四个异硫脲基通过硫醚键相连而成。该异硫脲基呈中度碱性，使阿利新蓝带阳离子。阿利新蓝使碳水化合物着色的确切机制不明，普遍认为是阳离子的异硫脲基通过静电与组织内的多聚阴离子相连。如含羧基和硫酸根的酸性黏液物质的羧基和硫酸根形成不溶性复合物，即染料分子中带正电荷的盐键和酸性黏液物质中带负电荷的酸性基团结合呈蓝色。
利用染液的不同pH 值可区分粘液物质的类属，pH 值为 2.5 时组织内的羧基电离， 带有一个负电荷， 与阿利新蓝中的阳离子形成盐键，是带有羧基的组织（如蛋白多糖/透明质酸以及上皮酸性黏蛋白）染色。pH 值为 1.0 时，组织内的硫酸根电离，带有一个负电荷，与阿利新蓝中的阳离子形成盐键，使带有硫酸根的组织（如硫酸黏液物质）染色。中性黏蛋白(如胃黏膜和 Brunner 腺体部位的中性黏蛋白）不能与阿利新蓝反应。
该试剂盒主要由阿利新蓝染液（pH1.0）和核固红复染液组成，只有硫酸粘液质着蓝色，核固红衬染细胞核呈红色。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
小鼠小肠石蜡切片
硫酸粘液质着蓝色，羧酸粘液质不着色，细胞核和背景着粉红色
硫酸粘液质着蓝色，羧酸粘液质不着色，细胞核和背景着粉红色
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2.8000
Publish_to：
Wiley Online Library
PMID：
30387555
Proteomic comparison between physiological degeneration and needle puncture model of disc generation disease
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Author：
He Shuangjun, Zhou Xinfeng, Yang Guotao, Zhou Zhangzhe, Zhang Yijian, Shao Xiaofeng, Liang Ting, Lv Nanning, Chen Jianhong, Qian Zhonglai
IF：
2.7210
Publish_to：
EUROPEAN SPINE JOURNAL
PMID：
35842490
The recombinant BMP-2 loaded silk fibroin microspheres improved the bone phenotype of mild osteogenesis imperfecta mice
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Author：
Ting Fu, Yi Liu, Zihan Wang, Yaqing Jing, Yuxia Zhao, Chenyi Shao, Zhe Lv, Guang Li
IF：
2.7000
Publish_to：
PeerJ
PMID：
37927786
Deer antler stem cells immortalization by modulation of hTERT and the small extracellular vesicles characters
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Author：
Peng Yinghua
IF：
2.6000
Publish_to：
Frontiers in Veterinary Science
PMID：
39430380
Facilitative role of circPVT1 in osteogenic differentiation potentials of bone marrow mesenchymal stem cells from patients with osteoporosis through the miR-30d-5p/ITGB3 axis
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Author：
Hongjian Tan, Yiwen Wang, Zehua Zou, Yufei Xing, Zuowei Shi, Kaifu Wang, Daming Dong
IF：
2.5860
Publish_to：
TISSUE & CELL
PMID：
35430412
Comparison of the differentiation abilities of bone marrow‑derived mesenchymal stem cells and adipose‑derived mesenchymal stem cells toward nucleus pulposus‑like cells in three‑dimensional culture
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Author：
Xuejun Dai, Yanyu Guan, Zhongzi Zhang, Ying Xiong, Chengwei Liu, Haifeng Li, Bailian Liu
IF：
2.4470
Publish_to：
Experimental and Therapeutic Medicine
PMID：
34373704
Effects of Oligomeric Proanthocyanidins on Cadmium-Induced Extracellular Matrix Damage via Inhibiting the ERK1/2 Signaling Pathway in Chicken Chondrocytes
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Author：
Jianhong Gu, Dan Liu, Anqing Gong, Xinrui Zhao, Jiatao Zhou, Panting Wang, Han Xia, Ruilong Song, Yonggang Ma, Hui Zou, Muhammad Azhar Memon, Yan Yuan, Xuezhong Liu, Jianchun Bian, Zongping Liu, Xishuai Tong
IF：
2.0000
Publish_to：
Veterinary Sciences
PMID：
40284819
A comprehensive stem cell laboratory module with blended learning for medical students at Tongji University
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Author：
Jie Xu, Zhihua Shao, Song Jia, Jihong Sha, Jiao Li, Furong Gao, Xiujuan Shi, Juan Wang, Caixia Jin, Mei Jiang, Haibin Tian, Jinfeng Cao, Hu Pu, Lei Xu, Lixia Lu
IF：
1.4000
Publish_to：
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
PMID：
38189805
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