抗荧光衰减封片剂(PVP,含DAPI)
英文名称:Mounting Medium,Antifading(PVP,with DAPI)
储存条件:2-8℃,avoid light,1 year


产品简介
英文名称 | Mounting Medium,Antifading(PVP,with DAPI) |
中文名称 | 抗荧光衰减封片剂(PVP,含DAPI) |
储存条件 | 2-8℃,avoid light,1 year |
规格 | 25ml |
单位 | 瓶 |
抗荧光衰减封片剂是一种用于减缓荧光衰减的封片试剂,在常规水性封片剂的基础上加入抗荧光衰减成分,有强烈的抗荧光衰减作用,用于对荧光成像的组织和细胞样品的封片。封片后,样品 4℃或者-20℃避光可保存 2-3 周。本试剂操作简单,在封片时用移液枪滴一滴抗荧光衰减封片液,盖上盖玻片就可以了。
抗荧光衰减封片剂内含 DAPI 即 4',6-二脒基-2-苯基吲哚(4',6-diamidino-2-phenylindole),是一种能够与 DNA 强力结合的荧光染料,常用与荧光显微镜观测。因为 DAPI 可以透过完整的细胞膜,它可以用于活细胞和固定细胞的核染色,显微镜下可以看到显蓝色荧光的细胞核。DAPI 和双链 DNA 结合后,最大激发波长为360nm,最大发射波长为460nm。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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