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英语
产品简介
| 中文名称 | GUS染色试剂盒(即用型) |
| 英文名称 | GUS Stain Kit，Ready-To-Use |
| 储存条件 | -20℃，避光保存，有效期6个月。 |
| 规格 | 20ml 100ml |
| 单位 | 盒 |
X-Gluc（X-GlcA）分子量为 521.8，CAS 号为 18656-96-7，是检测大肠杆菌中 GUS 基因的底物，可快速检测植物中 GUS 基因融合标记。GUS 染色液在适宜的反应条件下，β-葡萄糖苷酶(GUS)可将X-Gluc 水解成蓝色物质，该物质不溶解于转基因的细胞核组织中的靛蓝物质，具有 GUS 活性的部位或位点呈现蓝色或蓝色斑点，可用肉眼或显微镜观察到。GUS 染色液多用于转基因植物的 GUS基因表达分析。
该试剂盒为即用型，临用前按比例混合配制染色工作液，操作简便。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
拟南芥幼苗
阳性位点着蓝色，非阳性位点不着色
阳性位点着蓝色，非阳性位点不着色
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技术问答
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你好，请问未转基因的拟南芥也被染成染色的，会是什么原因2024-11-08 17:01:42
您好，可能有以下几种原因：1. 拟南芥存在微生物污染现象导致GUS染色阳性；2. 可能存在内源性类GUS酶，能催化GUS底物反应产生蓝色；3. 底物存在非特异性结合，可能与细胞内的某些多糖、蛋白质或其他生物大分子结合，在特定的细胞环境中引发颜色变化。
你好，我想问下这个试剂盒就是一瓶吗？能染烟草吗?2023-12-01 14:04:20
您好，该试剂盒为双组分试剂盒，即混即用，可用于转基因烟草叶片染色。
您好，我想问一下，拟南芥的幼苗染了一晚上，没有变蓝，是什么原因啊？拟南芥染色大概需要多长时间啊?2023-06-14 10:31:54
您好，通常过夜即可，正常未转基因组织一般不着色。