产品简介
别名 | 通用RT-PCR试剂盒(AMV) |
英文名称 | Universal RT-PCR Kit(AMV) |
储存条件 | Store at -20℃,1 year. |
规格 | 50T 100T |
单位 | 盒 |
本试剂盒适用于各种 RNA制品的反转录反应以及随后的PCR扩增。它采用AMV进行反转录反应,能够获得更长的反转录产物。同时,在20ul反转录体系和50ul PCR反应体系中,还可以一次性得到足够量的PCR产物用于后续的克隆实验。本试剂盒中配置的酶均为进口的酶。RT酶采用进口的AMV,所以cDNA更长,基因的信息保留得更完整!反转录过程中特异的RNase抑制剂可有效降低由于外源RNase污染而导致实验失败的风险。本试剂盒使用方便、快捷,可广泛用于cDNA克隆及目的基因检测等分子生物学实验。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
相关论文
更多
Effects of long-term exposure to tire wear particle leachate on life-cycle chronic toxicity and potential toxic mechanisms in the marine copepod Tigriopus japonicus
点击查看 >>
Author:
Kaiming Yang, Kui You, Yang Liu, Hao Zhou, Jingjing Zhan, Hongguang Cheng, Xianliang Yi
IF:
11.4000
Publish_to:
WATER RESEARCH
PMID:
40054281
A non-retinol retinoic acid receptor-γ (RAR-γ/NR1B3) selective agonist, tectorigenin, can effectively inhibit the ultraviolet A-induced skin damage
点击查看 >>
Author:
Xintong Dai, Jing Jin, Yan Jia, Kai Yang, Jingxia Han, Zhiyuan Zhang, Xiujuan Ding, Cheng Yao, Tao Sun, Caibin Zhu, Huijuan Liu
IF:
9.4730
Publish_to:
BRITISH JOURNAL OF PHARMACOLOGY
PMID:
35731978
Tryptophan-rich diet and its effects on Htr7+ Tregs in alleviating neuroinflammation and cognitive impairment induced by lipopolysaccharide
点击查看 >>
Author:
Xue Dinghao, Guo Xu, Liu Jingjing, Li Yanxiang, Liu Luyu, Liao Guosong, Zhang Mingru, Cao Jiangbei, Liu Yanhong, Lou Jingsheng, Li Hao, Mi Weidong, Wang Long, Fu Qiang
IF:
9.3000
Publish_to:
Journal of Neuroinflammation
PMID:
39334486
ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy
点击查看 >>
Author:
Suleixin Yang, Ka Hong Wong, Peng Hua, Chengwei He, Hua Yu, Dan Shao, Zhi Shi, Meiwan Chen
IF:
8.9470
Publish_to:
Acta Biomaterialia
PMID:
34879292
Effects of copper on non-specific immunity and antioxidant in the oriental river prawn (Macrobrachium nipponense)
点击查看 >>
Author:
Long Wang, Nan Wu, Yi Zhang, Guiling Wang, Sunyan Pu, Tianyu Guan, Chuankun Zhu, Hui Wang, Jiale Li
IF:
7.1290
Publish_to:
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
PMID:
35364505
Circ_0092012 knockdown restrains non-small cell lung cancer progression by inhibiting cell malignant phenotype and immune escape through microRNA-635/programmed death ligand 1 axis
点击查看 >>
Author:
Jin Yan, Jian Zhu, Xiaoli Zhu, Hailing Liu, Guoping Chen
IF:
6.8320
Publish_to:
Bioengineered
PMID:
35723188
Effects of cadmium on antioxidant and non-specific immunity of Macrobrachium nipponense
点击查看 >>
Author:
Long Wang, Jianbin Feng, Guiling Wang, Tianyu Guan, Chuankun Zhu, Jiale Li, Hui Wang
IF:
6.2910
Publish_to:
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
PMID:
34419645
Lactiplantibacillus plantarum fermentation enhanced the protective effect of kiwifruit on intestinal injury in rats: Based on mitochondrial morphology and function
点击查看 >>
Author:
Yun Ma, Xiao Chen, Ruiyu Xu, Hongyan Niu, Qun Huang, Yan Zhou
IF:
6.1000
Publish_to:
Food Chemistry-X
PMID:
38144866
MiR-29a-3p promotes nasal epithelial barrier dysfunction via direct targeting of CTNNB1-VCL module in allergic rhinitis
点击查看 >>
Author:
Na Wang, Pu Li, Junqi Liu, Zhenlin Wang
IF:
5.7140
Publish_to:
INTERNATIONAL IMMUNOPHARMACOLOGY
PMID:
37262956
Thymoquinone Ameliorates Carfilzomib-Induced Renal Impairment by Modulating Oxidative Stress Markers,Inflammatory/Apoptotic Mediators,and Augmenting Nrf2 in Rats
点击查看 >>
Author:
Marwa M. Qadri, Mohammad Firoz Alam, Zenat A. Khired, Reem O. Alaqi, Amani A. Khardali, Moudi M. Alasmari, Ahmad S. S. Alrashah, Hisham M. A. Muzafar, Abdullah M. Qahl
IF:
5.6000
Publish_to:
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID:
37445797
Distinct responses from triglyceride and cholesterol metabolism in common carp (Cyprinus carpio) upon environmental cadmium exposure
点击查看 >>
Author:
Wenli Guo, Beibei Gao, Xiaoqian Zhang, Quanzhong Ren, Dizhi Xie, Junping Liang, Hui Li, Xianfeng Wang, Yuru Zhang, Sijin Liu, Guoxing Nie
IF:
5.2020
Publish_to:
AQUATIC TOXICOLOGY
PMID:
35863253
miR-218-5p and miR-320a-5p as Biomarkers for Brain Disorders: Focus on the Major Depressive Disorder and Parkinson’s Disease
点击查看 >>
Author:
Wan Zhirong, Rasheed Madiha, Li Yumeng, Li Qin, Wang Peifu, Li Jilai, Chen Zixuan, Du Jichen, Deng Yulin
IF:
5.1000
Publish_to:
MOLECULAR NEUROBIOLOGY
PMID:
37329382
Aurantii fructus immaturus carbonisata-derived carbon dots and their anti-depression effect
点击查看 >>
Author:
Li Xiaopeng, Dai Ertong, Li Menghan, Kong Ruolan, Yuan Jinye, Li Tingjie, Wang Shuxian, Zhang Yue, Kong Hui, Zhao Yan
IF:
5.0000
Publish_to:
Frontiers in Molecular Biosciences
PMID:
38259687
Nicotinamide mononucleotide-elicited NAMPT signaling activation aggravated adjuvant-induced arthritis in rats by affecting peripheral immune cells differentiation
点击查看 >>
Author:
Qi-Hai Wang, Yan Li, De-Yu Dou, Rui Wang, Tian-Tian Jiang, Lin Wang, Ming-Qiang Li, Opeyemi Joshua Olatunji, Jian Zuo
IF:
4.9320
Publish_to:
INTERNATIONAL IMMUNOPHARMACOLOGY
PMID:
34130151
Immunomodulatory effects and multi-omics analysis of Codonopsis Pilosula Extract in septic rats
点击查看 >>
Author:
Zhaodi Xia, Gufeng Li, Yufei Zhai, Liguo Tong, Yilin Ru, Mengyao Wu, Jinming Hu, Mengyuan Wang, Yaxi Meng, Bao Sun, Chunliu Wang, Xianlin Luo, Yidi Liu, Ye Zhao, Xiaohui Zheng, Pu Jia
IF:
4.8000
Publish_to:
JOURNAL OF ETHNOPHARMACOLOGY
PMID:
39368762
CircTMEM87A promotes the tumorigenesis of gastric cancer by regulating the miR-1276/SLC7A11 axis
点击查看 >>
Author:
Xueguang Dong, Xiumei Chen, Yuanhao Zhao, Qunyan Wu, Yuguo Ren
IF:
4.1000
Publish_to:
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
PMID:
38037531
Effect of astaxanthin on neuron damage, inflammatory factors expressions and oxidative stress in mice with subarachnoid hemorrhage
点击查看 >>
Author:
Yu Qian, Xinyu Lu, Lulu Chen, Jinyu Sun, Kan Cao, Qiang Yu, Junfei Shao
IF:
4.0600
Publish_to:
American Journal of Translational Research
PMID:
34956522
PRDX4 mitigates diabetic retinopathy by inhibiting reactive gliosis, apoptosis, ER stress, oxidative stress, and mitochondrial dysfunction in Müller cells
点击查看 >>
Author:
Yue Huang, Yuting Zhang, Yuan Liu, Yinan Jin, Hongwei Yang
IF:
4.0000
Publish_to:
JOURNAL OF BIOLOGICAL CHEMISTRY
PMID:
39706273
Circ-DTL sponges miR-758-3p to accelerate cervical cancer malignant progression by regulating DCUN1D1 expression
点击查看 >>
Author:
Xiaoning Luo, Jiewen Liu, Xiangcai Wang, Jun Yuan, Yu Zhang
IF:
3.6000
Publish_to:
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
PMID:
37522575
Circ_0001387 regulates SKA2 to accelerate breast cancer progression through miR-136-5p
点击查看 >>
Author:
Youyi Xiong, Lin Li, Nan Wang, Fang Wang, Zhuo Chen, Luhong Han, Shan Jiang, Yuanting Gu
IF:
3.2230
Publish_to:
Thoracic Cancer
PMID:
37132052
BMSC-derived exosomal miR-219-5p alleviates ferroptosis in neuronal cells caused by spinal cord injury via the UBE2Z/NRF2 pathway
点击查看 >>
Author:
Junjie Dong, Zhiqiang Gong, Hangchuan Bi, Jin Yang, Bing Wang, Kaili Du, Chunqiang Zhang, Lingqiang Chen
IF:
2.9000
Publish_to:
NEUROSCIENCE
PMID:
39084457
MicroRNA-34a negatively regulates Netrin1 and mediates MEK/ERK pathway to regulate chemosensitivity of gastric cancer cells
点击查看 >>
Author:
Liu Haiping, Wang Limin
IF:
2.8000
Publish_to:
Discover Oncology
PMID:
39404782
Mechanism of protection from insulin resistance by toll-like receptor 2 deficiency in high-fat diet fed mice: involvement in macrophage polarization
点击查看 >>
Author:
Ma Yu, Liu Xinyue, Wu Yaliu, Wang Wendong, Chang Xiaotong
IF:
2.7420
Publish_to:
MOLECULAR BIOLOGY REPORTS
PMID:
35034283
Tauroursodeoxycholic acid suppresses biliary epithelial cell apoptosis and endoplasmic reticulum stress by miR-107/NCK1 axis in a FXR-dependent manner
点击查看 >>
Author:
Debiao Pan, Jun Wang, Hailin Ye, Yong Qin, Shengqian Xu, Guanxiong Ye, Hejuan Shen
IF:
2.6000
Publish_to:
DRUG AND CHEMICAL TOXICOLOGY
PMID:
38192027
Circ_0003789 Knockdown Inhibits Tumor Progression by miR-429/ZFP36L2 Axis in Gastric Cancer
点击查看 >>
Author:
Wan Lu, Jia Yu, Chen Na, Zheng Sen
IF:
2.4000
Publish_to:
BIOCHEMICAL GENETICS
PMID:
37962691
Expression of lncRNA APF in Peripheral Blood of Patients with Acute Myocardial Infarction Caused by Coronary Heart Disease and its Clinical Significance
点击查看 >>
Author:
Haitao Zeng, Fangxing Hu, Yong Duan, Hui Li, Yong Wang
IF:
1.8230
Publish_to:
International Heart Journal
PMID:
35831141
技术问答
(0)
我要评论

注意:
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。