超氧化物歧化酶(SOD)分型活性检测试剂盒(测Cu-Zn、Mn、总)
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Superoxide Dismutase (SOD) Typed Activity Assay Kit (WST colorimetry)
储存条件:Store at 2-8℃,6 months


产品简介
英文名称 | Superoxide Dismutase (SOD) Typed Activity Assay Kit (WST colorimetry) |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at 2-8℃,6 months |
运输条件 | 冷藏运输 |
规格 | 100T/48S |
单位 | 盒 |
SOD(EC 1.15.1.1)广泛存在于动物、植物、微生物和培养细胞中,根据其辅基结合金属离子的不同,可分为铜锌SOD、锰SOD等类型,铜锌SOD主要位于细胞质,锰SOD主要位于线粒体。SOD催化超氧化物阴离子发生岐化作用,生成H2O2和O2。SOD不仅是超氧化物阴离子清除酶,也是H2O2主要生成酶,在生物抗氧化系统中具有重要作用。
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子(O2-),O2-可与WST-1反应生成水溶性黄色甲臜,后者在450nm处有吸收峰;SOD可清除O2-,从而抑制了甲臜的形成;反应液黄色越深,说明SOD活性愈低,反之活性越高。样本经过处理后铜锌SOD活性不变,锰SOD活性丧失。通过测定总SOD活性和铜锌SOD活性,可计算得到锰SOD活性。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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