乙酰胆碱酯酶(AchE)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Micro Acetylcholinesterase(AchE) Assay Kit
储存条件:Store at 2-8℃,6 months


产品简介
中文名称 | 乙酰胆碱酯酶(AchE)活性检测试剂盒 |
英文名称 | Micro Acetylcholinesterase(AchE) Assay Kit |
别名 | 乙酰胆碱酯酶试剂盒 AchE Kit 乙酰胆碱酯酶(AchE)试剂盒 乙酰胆碱酯酶(AchE)测试盒 |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at 2-8℃,6 months |
规格 | 100T/48S |
单位 | 盒 |
测定意义:AchE属于丝氨酸水解酶,广泛存在于各种动物组织和血清中。AchE催化乙酰胆碱(Ach)水解,在神经传导调节中起重要作用。
测定原理:AchE催化Ach水解生成胆碱,胆碱与二硫对硝基苯甲酸(DTNB)作用生成5-巯基-硝基苯甲酸(TNB);TNB在412nm处有吸收峰,通过测定412 nm吸光度增加速率,计算AchE活性。
需要的仪器,耗材:紫外分光光度计/酶标仪、微量石英比色皿/96孔板、低温离心机、水浴锅、可调式移液枪和蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Peiyu Sun, Shunhe Wang, Ling Hu, Yinhu Huang, Yaping Wang
IF:
1.0000
Publish_to:
ACTAS ESPANOLAS DE PSIQUIATRIA
PMID:
39801411
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