| 产品类型 |
多肽 |
| CAS |
No.108433-95-0 |
| 单字母序列 |
GIGKFLHSAKKFGKAFVGEIMNS |
| 三字母序列 |
Gly-Ile-Gly-Lys-Phe-Leu-His-Ser-Ala-Lys-Lys-Phe-Gly-Lys-Ala-Phe-Val-Gly-Glu-Ile-Met-Asn-Ser |
| 别名 |
蛙皮素 2;Magainin II;Magainin 2 |
| 分子式 |
C114H180N30O29S1 |
| 分子量 |
2466.93 |
| 纯度 |
≥95% |
| 外观(性状) |
冻干粉;Lyophilized powder |
| 溶解性 |
H2O：50 mg/mL;
*超声助溶; |
| 盐体系 |
TFA盐;Trifluoroacetate salt |
| 来源 |
合成;Synthetic |
| 储存条件 |
Store at -20℃，2 years.(Avoid freeze/thaw cycles) |
| 背景说明 |
Magainin 2 (Magainin II) 是一种抗菌肽 (AMP)，从非洲爪蟾皮肤中分离得到；Magainin 2 显示抗生素活性对许多革兰氏阴性和革兰氏阳性细菌；Magainin 2 对原生动物也有活性；Magainin 2 通过优先与细菌膜中丰富的阴离子磷脂相互作用而发挥细胞毒性作用。
Magainin 2 (Magainin II) is an antimicrobial peptide (AMP) isolated from the skin of the African clawed frog Xenopus laevis. Magainin 2 displays antibiotic activity against numerous gram-negative and gram-positive bacteria. Magainin 2 also is active against protozoa[1]. Magainin 2 exerts its cytotoxicity effects by preferential interactions with anionic phospholipids abundant in bacterial membranes. |
| In Vitro |
Magainin 2 exhibits bactericidal effects and induces morphological changes in Escherichia coli regarding early apoptosis. Magainin 2 induces the expression of a bacterial protein with affinity for the caspase substrate and effects the expression of RecA as a caspase-like protein.
Magainin 2 kill bacteria by permeabilizing the cell membranes without exhibiting significant toxicity against mammalian cells. The main target of the peptide is considered to be the lipid matrix of the membranes. Application of 10 μg /mL magainin 2 to Paramecium caudatum, a protozoan, in pond water caused an osmotic swelling of the cell and a subsequent cell burst, suggesting that the peptide could perturb membrane functions responsible for osmotic balance.
Magainin 2 permeabilizes bacterial and mammalian membranes in significantly different ways. The peptide forms pores with a diameter of about 2.8 nm (less than 6.6 nm) in B. megaterium, and translocates into the cytosol. In contrast, the peptide significantly perturbes the membrane of CHO-K1 cells, permitting the entry of a large molecule (larger than 23 nm) into the cytosol, accompanied by membrane budding and lipid flip-flop, mainly accumulating in mitochondria and nuclei. |
| 参考文献 |
[1] Zasloff, M. Proc. Natl. Acad. Sci. USA. 84, 5449 (1987);
[2] Cruciani, RA. et al. EJPMOL 8, 187 (1992);
[3] Corzo, G. et al. Biochem. J. 359, 35 (2001);
[4] Matsuzaki, K. et al. Biochem. 36, 2104 (1997). |
| 研究领域 |
免疫学 |
| 规格 |
1mg 5mg 10mg |
| 单位 |
瓶 |
| 分类属性 |
目录肽 |
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