谷胱甘肽还原酶(GR)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Glutathione Reductases(GR) Activity Assay Kit
储存条件:Store at 2-8℃,6 months


产品简介
中文名称 | 谷胱甘肽还原酶(GR)活性检测试剂盒 |
英文名称 | Glutathione Reductases(GR) Activity Assay Kit |
别名 | 谷胱甘肽还原酶试剂盒 GR Kit 谷胱甘肽还原酶(GR)试剂盒 谷胱甘肽还原酶(GR)测试盒 |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at 2-8℃,6 months |
推荐 | 若使用96孔板测定,需使用96孔UV板,推荐货号YA0602 |
规格 | 100T/96S |
单位 | 盒 |
测定意义:GR是广泛存在于真核和原核生物中的一种黄素蛋白氧化还原酶,是谷胱甘肽氧化还原循环的关键酶之一(通常昆虫中GR被TrxR取代)。GR催化NADPH还原GSSG生成GSH,有助于维持体内GSH/GSSG比值。GR在氧化胁迫反应中对活性氧清除起关键作用,此外GR还参与抗坏血酸-谷胱甘肽循环途径。
测定原理:GR能催化NADPH还原GSSG再生GSH,同时NADPH脱氢生成NADP+;NADPH在340 nm有特征吸收峰,相反NADP+在该波长无吸收峰;通过测定340 nm吸光度下降速率来测定NADPH脱氢速率,从而计算GR活性。
需要的仪器,耗材:低温离心机、水浴锅、移液器、紫外分光光度计/酶标仪、微量石英比色皿/96孔板、和蒸馏水
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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