中文
英语
磷酸果糖激酶(PFK)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Phosphofructokinase(PFK) Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 磷酸果糖激酶(PFK)活性检测试剂盒 |
| 英文名称 | Phosphofructokinase(PFK) Activity Assay Kit |
| 检测方法 | 微量法 Spectrophotometer/Microplate Reader |
| 储存条件 | Store at -20℃，6 months |
| 推荐 | 若使用96孔板测定，需使用96孔UV板，推荐货号YA0602 |
| 规格 | 100T/96S |
| 单位 | 盒 |
测定意义：PFK（EC 2.7.1.11）广泛存在于动物、植物、微生物和培养细胞中，负责将果糖-6-磷酸和ATP转化为果糖-1,6二磷酸和ADP，是糖酵解过程的关键调节酶之一。
测定原理：PFK催化果糖-6-磷酸和ATP生成果糖-1,6-二磷酸和ADP，丙酮酸激酶和乳酸脱氢酶进一步依次催化NADH氧化生成NAD+，在340nm下测定NADH下降速率，即可反映PFK活性。
需要的仪器，耗材:紫外分光光度计/酶标仪、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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