2×Taq PCR MasterMix (含染料)
储存条件:Store at -20℃,1 year.
英文名称:2 × Taq PCR Mastermix (With Dyes)


产品简介
别名 | PCR扩增试剂盒 |
英文名称 | 2 × Taq PCR Mastermix (With Dyes) |
外观(性状) | 液体 |
储存条件 | Store at -20℃,1 year. |
规格 | 1ml 5*1ml |
单位 | 支 |
生化试剂,用于核酸电泳实验。
产品简介:
索莱宝公司生产的PCR MasterMix包含三种酶(Taq,Pfu,Taq plus),每种酶对应着两种PCR MasterMix(含染料和不含染料),一共六种PCR MasterMix。
Taq:扩增效率最高的耐热DNA聚合酶,其扩增速度约为1-2kb/min。扩增碱基出错率为10-5左右。其产物未端带有A,可直接用于TA克隆。
Pfu:目前保真度最高的耐热DNA聚合酶,碱基出错率为10-6,但扩增效率低于Taq酶,一般能很好的扩增2kb以下的片段。其扩增速度约为500bp/min左右。其产物为平未端,须加A后才可用于TA克隆。
Taq Plus:Taq和Pfu的等比混合物。集扩增效率高和保真度好于一身。扩增效率比Pfu高,保真度比Taq好。其扩增速度约为1000bp/min左右。其产物未端带有A,可直接用于TA克隆。 含染料PCR MasterMix反应完后可以直接电泳检测。不含染料的PCR MasterMix反应完后加入上样缓冲液后电泳检测。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Jin Xin, Xu Hainan, Hu Qing, Yin Yitong, Qin Meiying, Xia Zhijun
IF:
2.3000
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HISTOCHEMISTRY AND CELL BIOLOGY
PMID:
37874337
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Yongbin Jing, Mingkun Jia, Jinpeng Zhuang, Dong Han, Changlong Zhou, Jinglong Yan
IF:
2.2640
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BIOLOGICAL & PHARMACEUTICAL BULLETIN
PMID:
36450527
PAR2 Promoter Hypomethylation Regulates PAR2 Gene Expression and Promotes Lung Adenocarcinoma Cell Progression
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Kuan Wu, Lei Xu, Ling Cheng
IF:
2.2380
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Computational and Mathematical Methods in Medicine
PMID:
33968158
Knockdown of circ-Gatad1 alleviates LPS induced HK2 cell injury via targeting miR-22-3p/TRPM7 axis in septic acute kidney
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Zhang Pan, Guo Enwei, Xu Limin, Shen Zhenhua, Jiang Na, Liu Xinhui
IF:
2.2000
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BMC Nephrology
PMID:
38443846
Rab11a promotes the malignant progression of ovarian cancer by inducing autophagy
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Wang Yazhuo, Ren Yanan, Li Na, Zhao Jing, Zhao Sufen
IF:
2.1640
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Genes & Genomics
PMID:
36125654
Krüppel-like factor 1 serves as a facilitator in gastric cancer progression via activating the Wnt/β-catenin pathway
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Shaoyi Li, Yong Li, Bibo Tan, Zhaojie An
IF:
2.1490
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ACTA BIOCHIMICA POLONICA
PMID:
34669363
Updating specific PCR primer for detection of Cryptocaryon irritans from reared Larimichthys polyactis
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Xiao Xie, Chao Zheng, Aysha Zahid, Jindong Kong, Bushra, Dong Qian, Fei Yin, Bao Lou
IF:
2.0110
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EXPERIMENTAL PARASITOLOGY
PMID:
33549536
Inhibition of VDAC1 prevents oxidative stress and apoptosis induced by bisphenol A in spermatogonia via AMPK/mTOR signaling pathway
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Haixu Wang, Yan Li, Chuang Liu, Tianxiang Lu, Qian Zhai, Hongna Wang, Jianfang Zhang
IF:
2.0000
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JOURNAL OF TOXICOLOGICAL SCIENCES
PMID:
36858637
Calcium and integrin binding protein 1 (CIB1) induces myocardial fibrosis in myocardial infarction via regulating the PI3K/Akt pathway
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Guangquan Hu, Xiaojie Ding, Feng Gao, Jiehua Li
IF:
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EXPERIMENTAL ANIMALS
PMID:
34349085
Beneficial effects of Aucubin on restoration of rabbits with cartilage defect
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Zhao Gaofeng, Jiang Haiyue
IF:
1.7520
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CELL AND TISSUE BANKING
PMID:
35384569
Green synthesis of chitosan–phytic acid polymers and nanoparticles
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Author:
Jiahui Han1, Di Jia, Hao Yao , Ting Lv , Xi Xu , Xin Ge
IF:
1.5000
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FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
PMID:
37435898
Integrated physiological and transcriptomic analysis reveals mechanism of leaf in Phellodendron Chinense Schneid seedlings response to drought stress
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Lijuan Wu, Xiaoqing Lao, Yazeng Wu, Jing Zhang, Manyu Liang, Yanhong Yang, Xiaowei Gao
IF:
1.4000
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BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
PMID:
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