| 基因名 |
DOM3Z |
| 别名 |
Dom 3 homolog Z;DOM 3Z;Dom-3 homolog Z;DOM3 C. elegans homolog of Z;Dom3 homolog Z;DOM3L;Dom3z;DOM3Z_HUMAN;NG6;Protein Dom3Z;RAI1;RAT1-INTERACTING PROTEIN 1;DOM3Z |
| 英文名称 |
Recombinant human DOM3Z protein |
| 全名 |
dom-3 homolog Z (C. elegans) |
| 中文名称 |
dom-3同系物Z |
| 外观(性状) |
Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. (Or liquid with 5% glycerol.) |
| 纯度 |
≥85% by SDS-PAGE. |
| 储存条件 |
Reconstituted protein solution can be stored at 4-7°C for 1-2 weeks, stored at < -20°C for 1 year. |
| 运输条件 |
冷藏运输 |
| 浓度 |
1mg/ml by SDS-PAGE. |
| Swiss Prot |
O77932 |
| Gene ID |
1797 |
| 交叉反应 |
Human Mouse Rat (Bovine) |
| 分子量 |
24kDa |
| 区间范围 |
1-199aa |
| 背景说明 |
Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA (PubMed:28283058).The NAD-cap is present at the 5'-end of some RNAs and snoRNAs (PubMed:28283058).In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (PubMed:28283058).Preferentially acts on NAD-capped transcripts in response to environmental stress (PubMed:31101919).Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation (By similarity).Specifically degrades pre-mRNAs with a defective 5'-end m7G cap and is part of a pre-mRNA capping quality control (By similarity).Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1) (PubMed:29601584).In contrast to canonical decapping enzymes DCP2 and NUDT16, which cleave the cap within the triphosphate linkage, the decapping activity releases the entire cap structure GpppN and a 5'-end monophosphate RNA (By similarity).Also has 5'-3' exoribonuclease activities: The 5'-end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs (PubMed:29601584).Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates (By similarity). |
| 应用 |
Immunology research |
| 来源 |
E.coli |
| 纯化方法 |
NI-NTA affinity purification |
| 溶剂 |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 内毒素 |
Not measured |
| 生物活性 |
NO |
| 标签信息 |
With a 6×His tag at the N/C-terminus. |
| 规格 |
10ug 50ug 100ug 1mg |
| 单位 |
支 |
| 属性分类 |
重组抗原 |
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