| CAS |
No.210302-17-3 |
| 英文名称 |
BAM15 |
| 别名 |
;BAM15;BAM-15; |
| 分子式 |
C16H10F2N6O |
| 分子量 |
340.29 |
| 溶解性 |
Soluble in DMSO |
| 纯度 |
≥98% |
| 外观(性状) |
Light yellow to yellow Solid |
| 储存条件 |
Powder:-20℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year |
| MDL |
MFCD00373912 |
| SMILES |
FC(C=CC=C1)=C1NC2=NC3=NON=C3N=C2NC4=C(C=CC=C4)F |
| 靶点 |
Others |
| 通路 |
Others |
| 背景说明 |
BAM15是一种新型的 mitochondrial protonophore 的解偶联剂,也是一种有效的 AMPK 激活剂。 |
| 生物活性 |
BAM 15 is a mitochondrial protonophore uncoupler. BAM 15 is an oxidative phosphorylation (OXPHOS) uncoupler.[1] |
| In Vitro |
BAM 15 is able to increase O2 consumption across a broad dosing range without increasing ROS. BAM 15 and FCCP are structurally unrelated and it is observed that low doses of BAM 15 from 100 nM to 1 μM increase cellular O2 consumption rate (OCR) to a similar degree as FCCP， but higher concentrations from 1 μM to 50 μM reveal that BAM 15 is able to maintain uncoupled respiration at a high rate in a range of cell lines. BAM 15 is fully capable of increasing mitochondrial respiration in the presence of oligomycin and does so across a broader concentration range than FCCP in both myoblasts and hepatocytes. BAM 15 induces mitochondrial swelling， demonstrating that BAM 15 is a protonophore. BAM15-treated cells are more viable than FCCP-treated cells when administered across a broad dosing range up to 50 μM[1]. |
| In Vivo |
Compare to vehicle-treated mice， animals that receive BAM 15 are protected from kidney injury as indicated by lower plasma creatinine levels at 24 and 48 h post-ischemia， reduced tubular necrosis， less depletion of brush border villi， less obstruction of proximal tubules， and less immune cell infiltration[1]. |
| 细胞实验 |
L6 cells are incubated with the fluorescent indicator of mitochondrial membrane potential tetramethylrhodamine (TMRM， 125 nM) or DMSO (1%) control for 30 min. The cells are then centrifuged for 5 min at 700×g and resuspended in unbuffered DMEM at a concentration of 1×105 cells/mL. The cells are then treated with BAM 15 or DMSO (0.1%) for 10 min prior to flow cytometric analysis[1]. |
| 动物实验 |
Male mice (8-week old， C57BL/6) are used. Mice are i.p. injected with BAM 15 at 1 or 5 mg/kg， 1 h before kidney IR. Vehicle mice are also injected with the same solution BAM 15 is prepared with (3% DMSO in 50% PEG400)[1]. |
| 激酶实验 |
Electron flow assays are performed. Briefly， 5 μg of mitochondrial protein in MAS is loaded into a 24-well tissue culture plate and centrifuged at 2000×g for 15 min at 4°C. Prior to the assay， mitochondria are incubated at 37°C for 10 mins in MAS containing 10 mM pyruvate， 2 mM malate， and 5 μM BAM 15 or FCCP. Rotenone (2 μM)， succinate (10 mM)， antimycin A (4 μM)， and N，N，N′，N′-tetramethyl-p-phenylenediamine (TMPD， 100 μM) plus ascorbate (10 mM) are added sequentially as indicated in the figure. N=3 wells/plate of a representative of 3 plates[1]. |
| 数据来源文献 |
[1]. Kenwood BM， et al. Identification of a novel mitochondrial uncoupler that does not depolarize the plasma membrane. Mol Metab. 2013 Nov 28;3(2):114-23. |
| 规格 |
1mg 5mg 10mg 25mg |
| 单位 |
支 |
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