乙酰胆碱酯酶(AchE)活性检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:Acetylcholinesterase(AchE) Activity Assay Kit
储存条件:Store at 2-8℃,6 months


产品简介
中文名称 | 乙酰胆碱酯酶(AchE)活性检测试剂盒 |
英文名称 | Acetylcholinesterase(AchE) Activity Assay Kit |
检测方法 | 可见分光光度法 Spectrophotometer |
储存条件 | Store at 2-8℃,6 months |
规格 | 50T/24S |
单位 | 盒 |
测定意义:AchE是一种丝氨酸水解酶,广泛存在于各种动物组织和血清中。在神经突触间隙中AchE通过催化水解神经递质乙酰胆碱(Ach)来维持神经冲动的正常传递,在生物神经传导中起重要作用。
测定原理:AchE催化Ach生成胆碱,胆碱与二硫对硝基苯甲酸(DTNB)作用生成5-巯基-硝基苯甲酸(TNB),TNB在412nm处有吸收峰,通过测定TNB的生成量,即可计算出AchE的活性。
实验中所需仪器及设备:可见分光光度计、低温离心机、水浴锅、可调式移液枪、1ml玻璃比色皿、双蒸水
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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