产品简介
英文名称 | TBE Buffer,10× |
中文名称 | 10×TBE缓冲液 |
储存条件 | RT,2 years |
注意事项 | 低温环境下易有沉淀析出,属于正常现象,65℃水浴加热振荡使其完全溶解即可。 |
规格 | 500ml 10*500ml |
单位 | 瓶 |
TBE 缓冲液是生物学中常使用的核酸电泳缓冲盐溶液,主要用于DNA的琼脂糖凝胶电泳。TBE的主要成分是Tris-硼酸盐与EDTA,缓冲能力强,适合较长时间电泳,分辨率较高,电泳小于1kb的片段时分离效果好。TBE 缓冲液中的硼酸成分会影响DNA回收效率以及后续的酶反应,若要进行DNA片段的琼脂糖凝胶电泳回收实验,建议使用TAE 缓冲液。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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技术问答
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这个10X TBE工作液浓度是0.5X,我是得稀释20倍吗?2022-12-26 22:26:12
您好,如果实验中的TBE的工作浓度是0.5x,那需要将原液稀释20倍。
10×TBE缓冲液无RNase污染吗?2022-09-30 08:58:19
您好,没有做过去除RNA酶的特殊处理。