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睾酮

CAS:No.58-22-0

英文名称:Testosterone

分子式:C19H28O2

分子量:288.42

储存条件:Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:HPLC≥98%

睾酮
小分子同系列3送1
货号:
IT0110
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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使用本产品的案例(仅供参考

In Vitro

Cell(HUMSCs;1 mM testosterone)

Male germ-like cell differentiation of HUMSCs(人类胚胎干细胞的雄性生殖样细胞分化)

The HUMSCs used in each experiment were from passages 3–5. The male germ-like cell differentiation medium consisted of HDMEM-F12 supplemented with 5% FBS, 50% fi ltered testicular cell conditioned medium, 2 mM all-trans retinoic acid and 1 mM testosterone (Solarbio,Beijing, China). HUMSCs were grown in differentiation medium for 7, 14, or 21 days; half of the differentiation induction medium was replaced every 3–4 days. HUMSCs of the control group were cultured in normal medium (DMEM-F12 containing 5% FBS and 2% penicillin/streptomycin).

来源文献:Xie L ,  Zhou G ,  Ma L , et al. Differential expression profiles of lncRNAs and mRNAs during male germ-like cell differentiation in human umbilical mesenchymal stem cells by RNA-seq[J].  2021.


Cell(epidermal cells and skin-derived precursors (SKPs),2 nM testosterone)

Formation of HFS and IHFS

After collected in isolation procedure, the epidermal cells and SKPs were mixed at ratio of 1:5e9. Then the mixture was seeded into 20 ml HF induction culture medium (DMEM/F12;10% (v/v) FBS, 1% (v/v) Insulin-transferrin-selenium,0.2 nM 3,30,5-Triiodo-L-thyronine, 20 ng/ml basic fibroblast growth factor, 2 nM hydrocortisone hemisuccinate, 2 nM testosterone, Solarbio, China; 20 nM b-estradiol, Solarbio, China; 20 nM progesterone, Solarbio, China) droplet on the lid of 100 mm dish with a total cell of 5 x105 in each droplet. When the lid was put upside down, the cells sedimented at the bottom of the droplet and aggregated to form HFS after 24 h culture. The culture medium was changed every other day. After 7 days culture, the HFS was collected.

来源文献:Zhang Y, Enhejirigala, Li Z, Song W, Yao B, Li J, Wang Y, Duan X, Yuan X, Huang S, Fu X. The role of CTHRC1 in hair follicle regenerative capacity restored by plantar dermis homogenate. Biochem Biophys Res Commun. 2021 Sep 24;571:14-19. doi: 10.1016/j.bbrc.2021.07.056. Epub 2021 Jul 20. PMID: 34298337.



备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

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