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噻唑蓝

CAS:No.298-93-1

英文名称:MTT

分子式:C18H16BrN5S

分子量:414.32

储存条件:Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:≥98%

噻唑蓝
满3999赠露营车
货号:
IM0280
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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MTT是一种广泛用于测量细胞增殖的比色剂。MTT可以被线粒体内的一些脱氢酶还原生成结晶状的深紫色产物formazan,可以被DMSO完全溶解,然后通过酶标仪可以测定490nm波长附近的吸光度。细胞增殖越多越快,则吸光度越高;细胞毒性越大,则吸光度越低。

注意事项:MTT溶液需避光保存,长时间光照会导致失效。当颜色变为灰绿色时,请勿使用。


使用说明(仅供参考

1.收集对数期细胞,根据自己的实验需求,培养细胞。
2.小心吸去上清,加入90μL新鲜培养基,再加入10μL MTT溶液,继续培养4 h。
3.然后吸掉上清,每孔加入110μL DMSO,置摇床上低速振荡10 min,使结晶物充分溶解。

4.在酶联免疫检测仪490 nm处测量各孔的吸光值。

注:MTT溶液浓度一般的常用浓度为0.5%(仅供参考),实验者也可根据自己的实验需求进行调整。


使用本产品的应用案例(仅供参考

In Vitro

Cell (HEK-293 cells,37 °C ,4 h,490nm)

Cell viability was determined by a quantitative colorimetric assay with MTT. To screen the pre-protection of SDAP1 and SDAP2, cells were pretreated with SDAP1 and SDAP2 (0.25, 0.5, 0.75, 1, 1.25, 1.5 mg/mL) before administration of GM (3 mg/mL) for 24 h. HEK-293 cells were dispensed in 96-well plates at a density of 8,000 cells/well and cultured at 37 °C for 24 h. Afer 24 h incubation, cells were treated with various concentrations of SDAP1 and SDAP2 (0.25, 0.5, 0.75, 1, 1.25 and 1 mg/mL) at 24 h, and aferwards exposed to GM (3 mg/mL) for 24 h. Next, afer 24 h of GM treatment, the MTT solution was added to each well, and incubated at 37 °C for 4 h. Te medium was removed, and 150 μL of dimethylsulfoxide (Solarbio, China) was added to each well. Finally, the optical density was detected with a microplate reader at 490 nm.

来源文献:Wang Z, Wang L, Wang J, Luo J, Ruan H, Zhang J. Purified Sika deer antler protein attenuates GM-induced nephrotoxicity by activating Nrf2 pathway and inhibiting NF-κB pathway. Sci Rep. 2020 Sep 24;10(1):15601. doi: 10.1038/s41598-020-71943-6. PMID: 32973191; PMCID: PMC7518274.


Cell(HepG2 Cell,5 mg/mL MTT,4h, 570nm

Methylthiazole tetrazolium (MTT) assay was applied to measure the cell viability of  Up-3, Up-4, and Up-5. Briefly, after incubation, the cells was washed with PBS, and  incubated with 10 µL of MTT (5 mg/mL) for 4 hours. The supernatants was mixed  with 100 µL of DMSO and then the absorbance was measured at 570 nm with a  microplate reader. The absorbance of the  untreated cells (Normal group) was considered as 100%.

来源文献:Zhong QW, Zhou TS, Qiu WH, Wang YK, Xu QL, Ke SZ, Wang SJ, Jin WH, Chen JW, Zhang HW, Wei B, Wang H. Characterization and hypoglycemic effects of sulfated polysaccharides derived from brown seaweed Undaria pinnatifida. Food Chem. 2021 Mar 30;341(Pt 1):128148. doi: 10.1016/j.foodchem.2020.128148. Epub 2020 Sep 22. PMID: 33038776.


Cell ( A549 or A549/DDP cells20 μL MTT (5 mg·mL−1) was added to the wells for 4 h in an incubator )

The MTT assay was used to evaluate in vitro cytotoxicity of the drugs. Briefly, A549 or A549/DDP cells were seeded into 96-well plates (5,000 cells per well) and treated with various concentrations of the drugs for 48 h. Then, 20 μL MTT (5 mg·mL−1) was added to the wells for 4 h in an incubator. The formazan formed from MTT was dissolved by adding 150 μL DMSO. 


来源文献:Ye W, Lv H, Zhang Q, Zhao J, Zhao X, Zhao G, Yan C, Sun F, Zhao Z, Jia X. A cisplatin and disulphiram co-loaded inclusion complex overcomes drug resistance by inhibiting cancer cell stemness in non-small cell lung cancer. J Drug Target. 2024 Dec;32(2):159-171. doi: 10.1080/1061186X.2023.2298844. Epub 2024 Feb 1. PMID: 38133515.



In Vivo

Mice(Female Balb/c,6–8 weeks, subcutaneous injection,20 μL of MTT solution (5 mg/mL) for 4 h)

4T1 cells were seeded onto 96-well plates at 8000 cells per well. After 24 h of cultivation, the media was removed. 0.2 mL of PTX injections, DP NPs, or DPB NPs, diluted with the incomplete medium, were added to the 96-well plates. The blank incomplete medium was the negative control.

After 72h co-incubation, each well was treated with 20 μL of MTT solution (5 mg/mL) for 4 h. After removing the medium, 0.2 mL DMSO was added to each well. The absorbance of each well was measured at 570 nm using a microplate reader.

文献来源:Li M, Chen M, Li P, Zhang Z, Yu H, Wang X. Enhanced transcytosis and therapeutic efficacy of paclitaxel nanoparticles: Pyridylboronic acid modification and sialic acid targeting. Colloids Surf B Biointerfaces. 2024 Nov 30;247:114417. doi: 10.1016/j.colsurfb.2024.114417. Epub ahead of print. PMID: 39631321.




备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
暂无详情
Zhong QW, et al. Food Chem. 2021 Mar 30;341(Pt 1):128148.
Ma L, et al. ACS Applied Materials & Interfaces, 2020.
Li M, et al. Colloids Surf B Biointerfaces. 2024 Nov 30;247:114417. doi: 10.1016/j.colsurfb.2024.114417.
注意:
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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