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左氧氟沙星半水

CAS:No.138199-71-0

英文名称:Levofloxacin Hemihydrate

分子式:C18H20FN3O4·1/2H2O

分子量:370.38

储存条件:Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:HPLC≥98%

左氧氟沙星半水
小分子同系列3送1
货号:
IL0090
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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是抗生素化合物,抑制细菌DNA旋转酶活性。


In Vitro

Drug Susceptibility Assaylevofloxacin solution 1mg/ml

Drug sensitivity was determined using REsazurine Microtiter Assay (REMA).Antibiotics including isoniazid powder (Solarbio), rifampin powder (Solarbio), ethambutol powder (Solarbio), streptomycin solution 1mg/ml, levofloxacin solution 1mg/ml , and ampicillin powder (Solarbio) were dispensed using serial dilutions in each well of a microplate suitable for fluorescence reading. M. smegmatis cultures were grown up to mid-exponential phase and then 100μL expo- nentially growing M. smegmatis cultures were inoculated into 96-well plates containing 100μL 7H9 medium complemented with drugs of different concentration in each well. The plates were sealed and incubated for 3 days at 37. Then 20 μL of

Alamar-Blue (Solarbio) was added to each well of the plates. After another day of incubation at 37, plates were read on amicroplate reader to determine the relative fluorescence intensity (excitation 535nm and emission 590nm). Positive control (cells without antibiotic) was set to determine the maximum fluorescence that could be obtained, and well without cells was set as a negative control.

文献来源:Wei J, Lu N, Li Z, Wu X, Jiang T, Xu L, Yang C, Guo S. The Mycobacterium tuberculosis CRISPR-Associated Cas1 Involves Persistence and Tolerance to Anti-Tubercular Drugs. Biomed Res Int. 2019 Apr 2;2019:7861695. doi: 10.1155/2019/7861695. PMID: 31061828; PMCID: PMC6466960.


In Vitro

M.smegmatis levofloxacin 20μg/mL

10% exponentially growing M. smegmatis diluted in 7H9 medium was treated with antibiotics for 24h or 48h. Antimicrobial concentrations were as follows: rifampin 5μg/mL; streptomycin 20μg/mL; ethambutol 20μg/mL; levofloxacin 20μg/mL. CFU counts were performed in triplicate as previously described. Meanwhile, 20μg of bacterial suspension was extracted and reinoculated in 180μg/mL fresh 7H9 medium supplemented with 20nM recombinant resuscitation-promoting factor E (RpfE) in 96-well microtiter plates to calculate most probable number (MPN). Plates were incubated at 37statically for 14 days until MPN calculated as standard procedure . The persistence of M. smegmatis was assessed using the resuscitation index (RI), calculated as log 10 (MPN) -log 10 (CFU) .

文献来源:Wei J, Lu N, Li Z, Wu X, Jiang T, Xu L, Yang C, Guo S. The Mycobacterium tuberculosis CRISPR-Associated Cas1 Involves Persistence and Tolerance to Anti-Tubercular Drugs. Biomed Res Int. 2019 Apr 2;2019:7861695. doi: 10.1155/2019/7861695. PMID: 31061828; PMCID: PMC6466960.


备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
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Wei J,et al.Biomed Res Int. 2019 Apr 2;2019:7861695. doi: 10.1155/2019/7861695.
Wei J,et al.Biomed Res Int. 2019 Apr 2;2019:7861695. doi: 10.1155/2019/7861695.
注意:
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
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