国内地区 :
欢迎光临,!登录 | 注册
产品中心

活动

ABTS Diammonium Salt

CAS:No.30931-67-0

英文名称:ABTS Diammonium Salt

分子式:C18H24N6O6S4

分子量:548.68

储存条件:Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:HPLC≥98%

ABTS Diammonium Salt
小分子同系列3送1
货号:
IA0010
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
IA0010-W-1ml 咨询 咨询 咨询 咨询
IA0010-500mg 咨询 咨询 咨询 咨询
IA0010-100mg 咨询 咨询 咨询 咨询
IA0010-1g 咨询 咨询 咨询 咨询
数量:
-
+
立即购买

是一种辣根过氧化物酶 (HRP) 的底物。ABTS 经氧化后生成稳 定的蓝绿色阳离子 ABTS 自由基,在 405 nm 或 734 nm 处有最大吸收峰。被测物质加入 ABTS 自由基溶液后,所 含抗氧化成分能与 ABTS 自由基发生反应而使反应体系褪色,405 nm 的吸光度下降,在一定范围内其吸光度的变 化与自由基被清除的程度成正比。通过测定吸光度下降的程度来反映样本清除 ABTS 自由基的能力。ABTS 法可用于亲水性和亲脂性物质抗氧化能力测定。


使用本产品的应用案例(仅供参考

ABTS Free Radical Scavenging Assay.(ABTS 自由基清除测定)

案例1

To be specific, ABTS (25 mg) was added to the potassium persulfate (6.5 mL, 2.45 mmol/L) solution. Subsequently, the prepared ABTS solution was stored (14 h) at a constant temperature (25 ℃).The maintenance of the absorbance (734 nm, 0.70 ± 0.10) was confirmed before the experiments. Then, fresh MPN-GAs (1.0 mL, 1 mg/mL) and the prepared ABTS (3 mL) solution were subjected to an incubation process (60 min). Final ABTS scavenging activities were evaluated through the transformation of measured absorbance (734 nm). 

来源文献:Chen J, Chen X, Zhou G, Xu X. New insights into the ultrasound impact on covalent reactions of myofibrillar protein. Ultrason Sonochem. 2022 Mar;84:105973. doi: 10.1016/j.ultsonch.2022.105973. Epub 2022 Mar 3. PMID: 35272240; PMCID: PMC8913343.


案例2

ABTS was dissolved in 25 mM Tris, pH 7.0, to a 7 mM concentration. The ABTS radical cation (ABTS+·) was produced by mixing the ABTS stock solution with 2.45 mM potassium persulfate at a ratio of 1:1 (v:v) following 12−16 h of incubation in the dark (4 °C). The solution was then diluted with Tris buffer to an absorbance of 0.70 (±0.02) at 734 nm to form the test reagent. The test sample (3 μL) was then mixed with 237 μL of the diluted ABTS solution. The mixture reacted against light at room temperature for 30 min, and the absorbance was recorded at 734 nm on a ultraviolet spectrometer (AE). The blank was made from 3 μL of Tris buffer and 237 μL of diluted ABTS solution (AB). The percentage of inhibition of ABTS+· was calculated using the formula:

rate of inhibition=[ (AB-AE)/AB ] X 100

where AB is the absorbance of the blank sample and AE is the absorbance of the mixtures.

来源文献:Ma J, Yao Q, Chen X, Lv C, Zang J, Zhao G. Weak Binding of Epigallocatechin to α-Lactalbumin Greatly Improves Its Stability and Uptake by Caco-2 Cells. J Agric Food Chem. 2021 Aug 4;69(30):8482-8491. doi: 10.1021/acs.jafc.1c03427. Epub 2021 Jul 21. PMID: 34286590.




备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
暂无详情
Ma J, et al. Agric Food Chem. 2021 Aug 4;69(30):8482-8491. doi: 10.1021/acs.jafc.1c03427.
Chen J, et al. Ultrason Sonochem. 2022 Mar;84:105973. doi: 10.1016/j.ultsonch.2022.105973.
注意:
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
为科研服务、为生命尽责
联系方式
地址: 北京市通州区马驹桥联东U谷85号
客服电话: 400-968-6088
技术邮箱: service@solarbio.com
采购邮箱: purchase@solarbio.com
销售邮箱: sales-china@solarbio.com
Copyright © 2004-2022 北京索莱宝科技有限公司. 版权所有.
 京ICP备12051307号-7 
京公网安备 11011202000391号