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小鼠干扰素γ检测试剂盒
英文名称:Mouse IFN-γ ELISA Kit
应用: ELISA
反应性:Mouse
样品类型:血清/血浆/细胞培养上清
灵敏度:15.63 pg/ml
储存条件:2-8℃，6 months
产品简介
| 中文名称 | 小鼠干扰素γ检测试剂盒 |
| 英文名称 | Mouse IFN-γ ELISA Kit |
| 别名 | IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon, gamma |
| 储存条件 | 2-8℃，6 months |
| 检测目标 | IFN-γ |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Mouse |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 检测范围 | 31.25-2000 pg/ml |
| 灵敏度 | 15.63 pg/ml |
| 特异性 | 不与小鼠 IL-1α、IL-2、IL-4、IL-5、IL-6、IL-7、IL-9、SCF、VEGF， 人 IFN-γ等反应。 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 反应时间 | 3h |
| Swiss Prot | P01580 |
| Gene ID | 15978 |
| 试剂盒类型 | 传统ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠IFN-γ单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠IFN-γ会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠IFN-γ抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠IFN-γ发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠IFN-γ，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠IFN-γ浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠IFN-γ的浓度。 |
| 规格 | 48T 96T |
| 应用 | ELISA |
| 疾病系列 | 肥胖研究相关因子 骨质疏松症研究相关因子 慢阻肺COPD研究相关因子 骨关节炎相关因子 神经系统疾病相关因子 糖尿病研究相关因子 系统性红斑狼疮相关因子 哮喘相关因子 心血管疾病相关因子 |
| 研究领域 | 神经学 干细胞 新陈代谢 肿瘤&癌症 免疫学 |
| 单位 | 盒 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
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Publish_to：
MICROBES AND INFECTION
PMID：
37423325
The Protective Effects of Iron Free Lactoferrin on Lipopolysaccharide-Induced Intestinal Inflammatory Injury via Modulating the NF-κB/PPAR Signaling Pathway
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Author：
Hongya Wu, Linlin Fan, Yanan Gao, Jiaqi Wang, Nan Zheng
IF：
5.5610
Publish_to：
Foods
PMID：
36359991
The disruption of LPS in Salmonella Typhimurium provides partial protection against Salmonella Choleraesuis as a live attenuated vaccine
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Author：
Guodong Zhou, Qifeng Ma, Quan Li, Shifeng Wang, Huoying Shi
IF：
5.5000
Publish_to：
VACCINE
PMID：
37666697
Structural characteristics of a low molecular weight velvet antler protein and the anti-tumor activity on S180 tumor-bearing mice
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Author：
Tian-qi Cao, Hui-xian An, Rong-jie Ma, Ke-yao Dai, Hai-yu Ji, An-jun Liu, Jia-ping Zhou
IF：
5.3070
Publish_to：
BIOORGANIC CHEMISTRY
PMID：
36463590
Comprehensive assessment of peptide derived from pig spleen: Preparation, bioactivity and structure-activity relationships
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Author：
Chao Liu, Wen-jie Ding, Yao Huo, An-jun Liu
IF：
5.2000
Publish_to：
Food Bioscience
PMID：
Integrated systems pharmacology and transcriptomics to dissect the mechanisms of Loki Zupa decoction in the treatment of murine allergic asthma
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Author：
Cong Xie, Aman Gul, Hang Yu, Xi Huang, Lingling Deng, Yue Pan, Shuangshuang Ni, Mammat Nurahmat, Muhammadjan Abduwaki, Qingli Luo, Jingcheng Dong
IF：
5.1950
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
35533913
NCBP1 Improves Cognitive Function in Mice by Reducing Oxidative Stress,Neuronal Loss,and Glial Activation After Status Epilepticus
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Author：
Gao Xiaoying, You Zhipeng, Huang Cong, Liu Zhixiong, Tan Zixiao, Li Jiran, Liu Yang, Liu Xingan, Wei Fan, Fan Zhijie, Qi Sihua, Sun Jiahang
IF：
5.1000
Publish_to：
MOLECULAR NEUROBIOLOGY
PMID：
37474884
Heterologous Prime-Boost Immunization with DNA Vaccine and Modified Recombinant Proteins Enhances Immune Response against Trueperella pyogenes in Mice
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Author：
Ting Huang, Kelei Zhao, Xuhao Song, Tao Song, Xinrong Wang, Xiuyue Zhang, Bisong Yue, Yiwen Chu
IF：
4.9610
Publish_to：
Vaccines
PMID：
35746448
Preparation of Water-Soluble Acetylaminoglucan with Low Molecular Weight and Its Anti-Tumor Activity on H22 Tumor-Bearing Mice
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Author：
Jinfang Zhang, Keyao Dai, Mei Li
IF：
4.9270
Publish_to：
MOLECULES
PMID：
36364099
Enhanced Pathogenic Consequences Induced by a Seven-Amino-Acid Extension in the G Protein of the HRSV BA9 Genotype
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Author：
Na Wang, Jingjing Song, Lei Cao, Naiying Mao, Yuqing Shi, Jie Jiang, Wuyang Zhu, Yan Zhang
IF：
4.9000
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
40076707
Manganese-mediated potentiation of antitumor immune responses by enhancing KLRG1+ Macrophage function
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Author：
Liyan Ge, Hui Guo, Wei Zhou, Weifeng Shi, Jiawei Yue, Yumin Wu
IF：
4.8000
Publish_to：
INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：
39153309
Sodium Alginate Hydrogel-Mediated Cancer Immunotherapy for Postoperative In Situ Recurrence and Metastasis
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Author：
Yingying Zhang, Tiange Wang, Yinping Zhuang, Tiandi He, Xiaoli Wu, Lin Su, Jun Kang, Jin Chang, Hanjie Wang
IF：
4.7490
Publish_to：
ACS Biomaterials Science & Engineering
PMID：
34757733
The Oncolytic virus VT1092M and an Anti-PD-L1 antibody synergize to induce systemic antitumor immunity in a murine bilateral tumor model
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Author：
Wei Zhu, Mingxia Shao, Chao Tian, Jianshuai Yang, Hua Zhou, Jiajia Liu, Chunyang Sun, Min Liu, Jinyu Wang, Lijun Wei, Shuzhen Li, Xiaopeng Li, Jingfeng Li
IF：
4.5000
Publish_to：
Translational Oncology
PMID：
38843659
The HSP90 inhibitor 17-DMAG alleviates primary biliary cholangitis via cholangiocyte necroptosis prevention
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Author：
Liuying Chen, Huiqian Fan, Huikuan Chu, Fan Du, Yixiong Chen, Lilin Hu, Zhonglin Li, Weijun Wang, Xiaohua Hou, Ling Yang
IF：
4.4800
Publish_to：
JOURNAL OF CELLULAR BIOCHEMISTRY
PMID：
36037317
Tumor cells induce neural DKK1 expression to promote MDSC infiltration and subsequent T cell suppression
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Author：
Ruoyan Liu, Xiaotian Shi, Shuangshuang Qian, Zhonghao Sun, Hao Dai, Yongwei Wu, Shihui Cao, Jingtao Luo, Ze Zhang
IF：
4.4000
Publish_to：
CELLULAR SIGNALLING
PMID：
39710089
Embelia Laeta aqueous extract suppresses acute inflammation via decreasing COX-2/iNOS expression and inhibiting NF-κB pathway
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Author：
Zhongmin Zhang, Li Li, Guoxin Huang, Tong Zhou, Xinyue Zhang, Xinxin Leng, Zhenxing Chen, Jiang Lin
IF：
4.3600
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
34461190
Protective effects of recombinant lactoferrin with different iron saturations on enteritis injury in young mice
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Author：
L.L. Fan, Q.Q. Yao, H.M. Wu, F. Wen, J.Q. Wang, H.Y. Li, N. Zheng
IF：
4.2250
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
35379462
Free Radical Scavenging Effect and Immunomodulatory Activity of Total Saponins Extract of Ginseng Fibrous Roots
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Author：
Peng Zhang, Dongyan Zhang, Chuanjie Ma, Ruxia Wang, Weili Wang
IF：
4.2000
Publish_to：
MOLECULES
PMID：
38930835
An mRNA vaccine candidate encoding cholera toxin subunit B and conserved antigens of influenza viruses confers cross-protection against influenza a viruses in adult and aged mice
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Author：
Liang Xu, Zhihao Yu, Yifan Xu, Qin Wang, Guiqin Wang, Benchi Li, Qian Weng, Yongxiang Yi, Junwei Li
IF：
4.1000
Publish_to：
Human Vaccines & Immunotherapeutics
PMID：
39957235
Novel target and cofactor repertoire for the transcriptional regulator JTY_0672 from Mycobacterium bovis BCG
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Author：
Hui Wang, Xiaotian Li, Shuxian Wang, Ren Fang, Jiayin Xing, Ruiying Wu, Chunhui Zhang, Zhaoli Li, Ningning Song
IF：
4.0000
Publish_to：
Frontiers in Microbiology
PMID：
39845031
mRNA-LNP vaccines combined with tPA signal sequence elicit strong protective immunity against Klebsiella pneumoniae
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Author：
Ting Huang, Siyou Che, Zheng Lv, Danrui Hao, Runyu Wang, Qinxuan Yi, Ling Mei, Yang Yuan, Hang Zou, Yidong Guo, Xinrong Wang, Yiwen Chu, Kelei Zhao
IF：
3.7000
Publish_to：
mSphere
PMID：
39745376
Berbamine and thymoquinone exert protective effects against immune-mediated liver injury via NF-κB dependent pathway
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Author：
Kathem Sarmed H., Abdulsahib Waleed K., Zalzala Munaf H.
IF：
3.4710
Publish_to：
Frontiers in Veterinary Science
PMID：
35958317
Characterization of genetic humanized mice with transgenic HLA DP401 or DRA but deficient in endogenous murine MHC class II genes upon Staphylococcus aureus pneumonia
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Author：
Feng Li, Bowen Niu, Lingling Liu, Mengmin Zhu, Hua Yang, Boyin Qin, Xiuhua Peng, Lixiang Chen, Chunhua Xu, Xiaohui Zhou
IF：
3.4000
Publish_to：
Animal Models and Experimental Medicine
PMID：
37246733
Knockdown of IFNAR2 reduces the inflammatory response in mouse model of type 1 diabetes
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Author：
Wei Sun, Jing Liu, Renhao Zhao, Teng Yang, Zhichen Zheng, Tongyu Zhang, Guofeng Wang
IF：
3.3220
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
35728283
Design a novel of Brucellosis preventive vaccine based on IgV_CTLA-4 and multiple epitopes via immunoinformatics approach
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Author：
Yueyue He, YueJie Zhu, Zhengwei Yin, Juan Shi, Kaiyu Shang, Tingting Tian, Huidong Shi, Jianbing Ding, Fengbo Zhang
IF：
3.3000
Publish_to：
MICROBIAL PATHOGENESIS
PMID：
39218373
Cordyceps cicadae ameliorates inflammatory responses, oxidative stress, and fibrosis by targeting the PI3K/mTOR-mediated autophagy pathway in the renal of MRL/lpr mice
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Author：
Feng Yang, Yanyan Zhang, Lei Dong, Zhichao Song
IF：
3.2000
Publish_to：
Immunity Inflammation and Disease
PMID：
38270299
Cryoablation plus chemotherapy regimen enhance anti-tumor immune response in a mouse model of Lewis lung cancer
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Author：
Hua Duan, Li Yang, Xueni Fang, Shaohua Yan, Yang Cao, Bingli Qiao, Tian Zhou, Kaiwen Hu
IF：
3.0000
Publish_to：
INTERNATIONAL JOURNAL OF HYPERTHERMIA
PMID：
39727085
More Ultrasound-Guided Percutaneous Microwave Ablation Leads to Higher Immune-Related Gene Expression and Boosts PD-1 Monoclonal Antibodies for Liver Cancer
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Author：
Ting Luo, Zhen Wang, Xiaoling Yu, Zhiyu Han, Zhigang Cheng, Fangyi Liu, Jie Yu, Ping Liang
IF：
2.9000
Publish_to：
ULTRASOUND IN MEDICINE AND BIOLOGY
PMID：
37867090
Immunomodulatory effects of selenium-enriched peptides from soybean in cyclophosphamide-induced immunosuppressed mice
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Author：
Jian Zhang, Siwei Gao, He Li, Mengdi Cao, Wenhui Li, Xinqi Liu
IF：
2.8630
Publish_to：
Food Science & Nutrition
PMID：
34760262
Recombinant-attenuated Salmonella enterica serovar Choleraesuis vector expressing the PlpE protein of Pasteurella multocida protects mice from lethal challenge
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Author：
Zhou Guodong, Tian Jiashuo, Tian Yichen, Ma Qifeng, Li Quan, Wang Shifeng, Shi Huoying
IF：
2.6000
Publish_to：
BMC Veterinary Research
PMID：
37598169
Effect of glabridin combined with bakuchiol on UVB-induced skin damage and its underlying mechanism: An experimental study
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Author：
Guanjie Peng, Yangsi Li, Yiyan Zeng, Bowen Sun, Lanyue Zhang, Qingqing Liu
IF：
2.3000
Publish_to：
Journal of Cosmetic Dermatology
PMID：
38497297
Ginsenoside Rh1 attenuates ovalbumin-induced asthma by regulating Th1/Th2 cytokines balance
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Author：
Li Qiang, Zhai Chunmiao, Wang Guodong, Zhou Jia, Li Weiguang, Xie Liquan, Shi Zhanli
IF：
2.0430
Publish_to：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
PMID：
34057179
Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs
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Author：
Yunyun Ma, Junjun Shao, Wei Liu, Shandian Gao, Guangqing Zhou, Xuefeng Qi, Huiyun Chang
IF：
2.0000
Publish_to：
Veterinary Sciences
PMID：
39852910
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