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英语
Fluo-4，AM 钙荧光探针Fluo-4，AM
英文名称:Fluo-4，AM
CAS:No.273221-67-3
分子式:C51H50F2N2O23
分子量:1096.94
储存条件:-20℃干燥避光保存，有效期1年。
产品简介
| 中文名称 | Fluo-4，AM 钙荧光探针Fluo-4，AM |
| 英文名称 | Fluo-4，AM |
| 别名 | 钙荧光探针Fluo-4, AM |
| CAS | No.273221-67-3 |
| 分子式 | C51H50F2N2O23 |
| 分子量 | 1096.94 |
| 外观(性状) | 冻干粉（橙红色） |
| 激发波长/发射波长(Ex/Em) | 494/516 |
| 储存条件 | -20℃干燥避光保存，有效期1年。 |
| 规格 | 100ug 1mg 10*100ug |
| 单位 | 瓶 |
Fluo-4是一种将Fluo-3结构中的Cl替换成F的钙荧光探针。由于将Cl替换成了电子吸引力更强的F，它的最大激发波长会向短波长处偏离10 nm左右。这个波长更接近于氩激光器的波长，所以用氩激光器激发时，Fluo-4的荧光强度比Fluo-3强一倍。
Fluo-4, AM穿透细胞膜进入细胞后被细胞内的酯酶剪切形成Fluo-4，从而被滞留在细胞内，Fluo-4若以游离配体形式存在时几乎是非荧光性的，但是当它与细胞内钙离子结合后可以产生较强的荧光，最大激发波长为494nm，最大发射波长为516nm。可以使用激光共聚焦显微镜或流式细胞仪检测细胞内钙离子浓度的变化。
HBSS：不含钙镁，不含酚红
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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FLuo-4染色胞内钙离子，该实验中是否需要用DAPI染细胞核作为细胞定位的对照2024-08-02 10:47:31
您好，DAPI复染细胞核这一步是可选的