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产品简介
| 英文名称 | TAE Buffer，50× |
| 外观(性状) | 无色液体 |
| 储存条件 | Store at RT，2 years. |
| 规格 | 500ml 10*500ml |
| 单位 | 瓶 |
TAE是一种电泳缓冲液，主要用于DNA分子的电泳。
TAE缓冲液 是生物学中使用最广泛的核酸电泳缓冲液，主要用于DNA 的琼脂糖凝胶电泳。TAE缓冲液的主要成分是Tris-乙酸盐与EDTA，电泳时双链线状DNA 的迁移率较快，电泳大于13kb 的片段时分离效果好，此外，回收DNA 片段时也宜用TAE 缓冲系统进行电泳。
TAE的缺点是缓冲容量小，长时间电泳（如过夜）不可选用，除非有循环装置使两极的缓冲液得到交换。
本产品为50×浓缩液，工作浓度为1×，可直接用蒸馏水稀释50 倍后使用。若产品出现沉淀析出，请置于37℃水浴使其溶解，不影响使用。
50×TAE Buffer 配制方法：
称量Tris 242g，Na2EDTA.2H2O 37.2g 于1L烧杯中；
向烧杯中加入约800ml去离子水，充分搅拌均匀；
加入57.1ml的冰乙酸，充分溶解；
加去离子水定容至1L后，室温保存。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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