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异硫氰酸荧光素的使用说明2025-03-05 10:39:47
您好，可参考产品说明书
能直接标记细菌吗，标记方法与蛋白有何不同2025-03-01 14:11:00
您好，异硫氰酸荧光素（FITC）可以直接标记细菌，其标记原理是 FITC 的异硫氰酸基能与细菌表面的氨基等基团发生反应，形成稳定的共价键，从而使细菌带上荧光标记。FITC 标记细菌和标记蛋白在标记对象、标记条件和标记后处理等方面存在一些不同，以下是具体介绍：1.标记对象方面：FITC 标记细菌时，主要是与细菌细胞壁或细胞膜表面的氨基、羟基等基团结合；FITC 标记蛋白是通过与蛋白分子中的氨基（主要是赖氨酸残基的 ε - 氨基）发生反应，形成硫脲键，从而将荧光素标记到蛋白分子上。2. 标记条件方面：标记细菌时，通常需要将细菌悬浮在适当的缓冲液中，如磷酸盐缓冲液（PBS）。由于细菌表面的基团性质和分布与蛋白不同，标记时的 pH 值、温度和反应时间等条件需要根据细菌的种类和特性进行优化。一般来说，标记细菌的 pH 值可能在 7 - 9 之间，温度在 4 - 37℃，反应时间在 30 分钟至数小时不等。标记蛋白时，反应体系通常也在缓冲液中进行，常用的缓冲液有 PBS 或碳酸盐缓冲液等。反应的 pH 值一般在 8 - 9 左右，温度通常为室温或 4℃，反应时间一般为 1 - 2 小时。但对于一些特殊的蛋白，可能需要调整缓冲液的成分、pH 值和反应时间等条件。3.标记后处理方面：标记后的细菌需要通过离心、洗涤等步骤去除未结合的 FITC。由于细菌是有生命的个体，在洗涤过程中要注意保持细菌的活性和形态完整，避免使用过于剧烈的离心条件或对细菌有损伤的洗涤液。标记后的蛋白也需要通过透析、凝胶过滤等方法去除未反应的 FITC。与细菌不同的是，蛋白在处理过程中更要注意保持其活性和结构稳定，避免高温、酸碱等因素导致蛋白变性。如果标记的蛋白需要进一步纯化，还可以采用离子交换层析、亲和层析等方法进行分离纯化。
这个有使用说明吗?2022-11-08 14:12:55
您好，产品使用详情可以拨打400-968-6088转2与我司技术支持取得联系。